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OBJECTIVE To investigate the anti-inflammatory effects and mechanism of Zhuang medicine Tongfeng li’an capsules on gouty arthritis in combination with in vivo and in vitro experiments. METHODS Sixty rats were randomly divided into normal group， model group， positive control group （27 mg/kg allopurinol+0.27 mg/kg colchicine）， Tongfeng li’an capsules low- dose， medium-dose and high-dose groups （2.2， 4.5， 9.0 g/kg）， with 10 rats in each group. Except for normal group， gouty arthritis model of rats was induced in other groups. Rats in each administration group were given corresponding drugs intragastrically， and rats in the normal group and model group were given equal volume of water intragastrically for 14 consecutive days. The degree of ankle joint swelling， serum level of interleukin-1β （IL-1β） and protein expressions of nuclear factor kappa-B （NF-κB） in synovial tissue were detected， and the histopathological changes of synovium tissue in the ankle joint of rats were observed. The inflammation model was established by stimulating RAW264.7 cells with lipopolysaccharide. After Tongfeng li’an capsules （62.5， 125， 250 μg/mL） were given， the levels of nitric oxide （NO）， reactive oxygen species （ROS） and IL-1β in the cells and protein expression of NF-κB were detected， and NF-κB localization in the cells was also determined. RESULTS Results of in vivo experiment showed that compared with normal group， the swelling degree of the ankle joint， serum IL-1β level and protein expression of NF-κB in synovium tissue were all increased significantly in model group （P＜0.05）； pathological changes such as synovial hyperplasia， edema， vascular congestion， capillary hyperplasia， and increased inflammatory cells were observed. Compared with model group， the levels of above indexes were all decreased significantly in Tongfeng li’an capsules high-dose group （P＜0.05）， and most of the above indexes were significantly reduced in Tongfeng li’an capsules medium-dose and low-dose groups （P＜0.05）； synovial hyperplasia of the ankle joint improved， and the infiltration of inflammatory cells 2019BS044） decreased. Results of in vitro experiment showed that Tongfeng li’an capsule could significantly reduce the levels of NO， ROS and IL-1β and protein expression of NF-κB（P＜0.01）， and inhibit NF- κB nucleation. CONCLUSIONS Tongfeng li’ancapsules have good anti-inflammatory effect on gouty arthritis， and its mechanism may be related to the inhibition of NF-κB signaling pathway activity.
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Objective To compare the particle pollution to the solution in the ampoule wrapped and broken by non-woven fabrics and medical gauze.Methods Totally 200 pcs of sterile water for injection(2 ml/pc) were randomly divided into the experimental group and the control group,with 100 pcs for each.For the experimental group,the ampoules were wrapped and broken by disinfected non-woven fabrics.For the control group,the ampoules were wrapped and broken by medical gauze.The solution was sucked from the ampoule with a 10 ml syringe and injected into a measuring cup for testing.Results The quantity of particles in the solution in the experimental group was less than that in the control group,and the difference was statistically significant (P＜0.05).Conclusion Wrapping and breaking the ampoule by disinfected non-woven fabrics can reduce particle pollution to the solution,and this method is easy to practice.
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Objective To study the correlation of uterine segment thickness and time limits for patients with prior cesarean section. Methods A total of 80 women with the first pregnancy after cesarean section in our hospital from April 2013 to February 2015,according to the interval of cesarean section and second pregnancy,were divided into group A (18 cases,interval ≤3 years),group B (23 cases,an in-terval of 3 to 6 years),group C (27 cases,an interval of 7 to 9 years),and group D (12 cases,the interval ≥9 years).The uterine segment thickness and the success rate of vaginal trial production were compared.Surgical failures underwent vaginal trial production due to cesarean section were observed.Results The uterine segment thickness values of group A and group B in were respectively (5.93 ±0.34)mm and (5.45 ±0.27)mm,which were significantly higher than group C (3.24 ±0.52)mm and group D (2.83 ±0.56)mm,the difference was statistically significant (P <0.05).The vaginal trial production success rates of group A and group B were respectively 88.89% (16 /18) and 78.26% (18 /23),which were significantly higher than group C 44.44% (12 /27)and group D 33.33% (4 /12)(P <0.05).The dos-age of oxytocin of group A was (35.34 ±4.32)mL,blood loss was (256.32 ±34.21)mL, The oxytocin hormone dosage of group B was (37.09 ±4.52)mL,blood loss was (260.11 ±35.53)mL,which were lower than those in group C and group D (P <0.05).The postpartum hemorrhage rate of group A,group B and group C was respectively 0(0 /2),20.00% (1 /5),33.33% (5 /15)lower than group D 87.50%(7 /8)(P <0.05).Conclusion Maternal uterus at cesarean section within 7 years about the degree of recovery are in good condition,the higher uterine segment thickness,this time period is good for subsequent pregnancy and childbirth.
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Aim Series of compounds,which were considered to be the antagonists of ET-1 receptor,were synthesized by Beijing Institute of Pharmacology and Toxicology.The biological activity of these compounds was screened and some active compounds were selected for further pharmacological characterization on pulmonary hypertension.Methods Radioligand binding assay was performed to study the binding affinity of compounds for ETA and ETB receptors.The biological activity of compounds was evaluated in isolated rat aortic ring and in systemic arterial pressure(SAP)of anesthetized rat experiments.In addition,hypotensive effect of compounds was investigated on monocrotaline induced pulmonary hypertension in rats.Results Compounds bind to ETA receptor had over 10 000 fold higher affinity than to ETB receptor.Contraction induced by ET-1 in isolated rat aortic ring was inhibited by compounds,and 1 ?mol?L-1 ETP-508 shifted the cumulative concentration-contraction response curve to ET-1 to right with no change in the maximal response.In vivo,the increase in SAP induced by ET-1 〔3.7 ?g?(0.5 ml)-1?kg-1〕 was inhibited by 2 mg?kg-1 compounds by intravenous infusion.Furthermore,BQ-485 and ETP-508 by intravenous infusion(0.4 mg?h-1)significantly inhibited 80 mg?kg-1(sc)monocrotaline induced pulmonary hypertension in rats.Conclusions These results indicate that ETP-508 and BQ-485 are highly selective ETA receptor antagonists and significantly inhibite monocrotaline induced pulmonary hypertension in rats.
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Objective:In order to build the method to apply Torpedo Acetylcholinesterase(AChE) labelled second antibody in ELBA. Methods:The labelling method is NaIO4-mediated glycoprotein oxidation method. Results:pH and temperature and the concentration of NaIO4 effect on labelling efficiency. The liter of AChE labelled IgG is higher than HPR labelled IgG. Conclusion:Torpedo Acetylcholinesterase-labelled second antibody prepared could be applied and replaced the HRP-labelled second antibody in ELISA.
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Tie effects of L-ascorbate 2-sulfate(AAS) on fatty liver and hyperli-pemia of rat and guinea pigs induced by various treatments were studied. The results showed that AAS lowered cholesterol and total lipid levels in the serum and liver of rats fed diets supplemented with lard and cholesterol, while ascorbic acid had no obvious effect. In guinea pigs, AAS not only lowered serum lipid values and liver cholesterol, triglyceride and total lipid levels of DDT treated animals, but also prevented from decline of body weights of these animals. It also had the same effects to lower the serum and liver lipid values of those animals fed pentabarbital or high lipid diet.Remarkable increases of the lipid peroxide value of animals given high doses of AsA were observed, but no such effect occurred in those fed AAS.
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Pressed biscuits were fortified with gelatin encapsulated VA powder, VC phosphate Mg and VB2 tetrabutyrate. The biological effects of these vita-rains were examined by human experiments. In 7 days of the experimental period, the subjects of the 1st group (10 male adults) took the fortified biscuit freely which contained 7500 IU VA powder, 5.16 mg B2 tetrabutyrate ( = 3mg VB2) and 308mg VC phosphate ( = 140 mgVC) in 750 g. The 2nd group took 250 g biscuit freely containing the same amount of vitamin and unfortified pressed biscuit. The 3rd group took only the unfortified ones freely. 5 days before or after fhe experimental period the subjects ate vitamin restricted diets. The nutritional status of VB2 was estimated by 24 hrs. urinary VB2 values, that of VC by urinary and fasting plasma VC levels, and that of VA by fasting plasma VA values.The urinary levels of VB2, VC and plasma values of VC of group 1 and 2 at the end of the experimental period were significantly higher than those of the pre-experimental period. They were also higher than those of group 3. On the 5th day of the post-experimental period, the status of VB2 and VC of group 1 and 2 was still better than that of group 3. Because the subjects were not in VA deficiency, the plasma levels of VA were kept steady.The stabilities of these vitamins in the fortified pressed biscuit were compared with those of ordinary vitamins. Stored in 85% RH 32℃ for 20 months, encapsulated VA powder retained 76%, VC phosphate 84%, but the ordinary VA only 14%, VC 29%. Stored in room temp. 75% RH for 2 years, the VA encapsulated powder retained 73%, VC phosphate 96%, but ordinary VA only 8%, VC 22.3%. In room temp. for 2 years, encapsulated VA powder retained 87%, VC phosphate almost did not lost, but ordinary VA retained 8%, VC retained 25% only. The stability of the VB2 tetrabutyrate was almost the same as that of VB2. It was shown by the guinea pig growth experiment that VC phosphate in pressed biscuit stored 20 months possessed the same biological effect as VC crystal.
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Male guinea pigs were placed on ordinary or high lipid diet for 30-36 weeks with various amounts of ascorbic acid (Vc) intake. The daily dose of low level intake was 0.7-1.2mg. The normal one was 2.5-5mg. The high dose was 30-45mg. The various amounts of Vc intake did not affect the cholesterol contents in serum and liver. The animals fed low level Vc were without "overt signs of deficiency, but morphological changes of aorta were developed in these animals either on ordinary or high lipid diet. On high lipid diet, the pathological changes of the vessels (aorta and arteriols in cardiac muscles) of animals fed low level of Vc were more severe than those of animals fed normal level, and those of animals fed high dose were the least severe.The result of this study suggested that the presence of a link between Vc and the integrity of the vessels. Prolonged low intake of YC may be regarded as being one of the causal factors in the development of arteriosclerosis. The requirements of Vc for subjects with high cholesterol level may be more than those of normal subjects.