ABSTRACT
OBJECTIVE: The aim of this study is to investigate the presence of human papillomavirus DNA and genotypes in breast cancer and normal breast tissue samples obtained from women from the northeast region of Brazil. METHOD: One hundred three breast cancer samples and 95 normal breast samples, as the non-malignant controls, were studied. DNA extraction was verified by human beta-globin gene amplification, and polymerase chain reaction was conducted based on HPV L1-specific consensus primers MY09/MY11 and GP5+/GP6+, followed by nested multiplex polymerase chain reaction with type-specific primers for the E6/E7 consensus region. RESULTS: Human papillomavirus DNA was detected in 51 (49.5%) breast carcinoma samples and 15 (15.8%) normal breast samples (p<0.0001). Human papillomavirus genotypes 6 and 11 were identified in 15.2% of all samples. CONCLUSIONS: The high frequency of human papillomavirus infection in breast cancer samples indicates a potential role of this virus in breast carcinogenesis in the studied participants.
Subject(s)
Humans , Female , Adult , Middle Aged , Aged , Aged, 80 and over , Breast Neoplasms/virology , DNA, Viral/genetics , Papillomavirus Infections/genetics , Case-Control Studies , Polymerase Chain Reaction , Cross-Sectional Studies , GenotypeABSTRACT
Epstein-Barr virus (EBV) has been associated with 10% of gastric carcinomas. The aim of this study was to determine the frequency of EBV in gastric carcinomas in Brazil assessed by in situ hybridization (ISH) and PCR, which would contribute to the characterization of the clinical and pathological aspects of EBV-associated gastric carcinomas. One hundred and ninety-two gastric carcinoma cases were collected at hospitals in two Brazilian states. Seventy-three out of 151 cases were PCR(+), while 11/160 cases were ISH(+). Nine out of eleven ISH(+) cases displayed a diffuse staining pattern and 2 out of 11 a focal pattern. Both techniques showed that the EBV(+) cases were characterized by their association with males, older patients, lower gastric region, intestinal type, advanced stage and poorly to moderately differentiated tumors. The concordance between the two techniques was 55.8% (Cohen's kappa index = 0.034). Four cases were ISH(+)/PCR(-), while 49 cases were PCR(+)/ISH(-). Only two cases showed stained lymphocytes by ISH and one of them was PCR(-). The observed discrepancy between the two techniques could not be explained just by the elevated accuracy of PCR. ISH(+)/PCR(-) carcinomas may be encountered if EBV is not present in the whole tumor tissue or if there are polymorphisms in the sequences of the viral genome amplified. On the other hand, the high frequency of PCR(+) results associated with the absence of ISH staining in lymphocytes and/or tumors cells suggests that the virus may be present in tumor cells or other cell types without expressing EBER1, the target of the ISH technique.
Subject(s)
Humans , Male , Carcinoma , Epstein-Barr Virus Infections , Gastrointestinal Tract , /genetics , /isolation & purification , In Situ Hybridization/methods , In Vitro Techniques , Polymerase Chain Reaction/methods , Tumor Cells, Cultured , Methods , Outpatients , MethodsABSTRACT
INTRODUÇÃO: O vírus Epstein-Barr (EBV) está associado a cerca de 10 por cento dos adenocarcinomas gástricos, representando mais de 50 mil casos por ano no mundo. Apesar dos estudos realizados em várias partes do mundo, alguns aspectos clinicopatológicos permanecem controversos. OBJETIVOS: O presente estudo teve como objetivo analisar as características clinicopatológicas de casos de adenocarcinomas gástricos procedentes dos estados de São Paulo e Ceará, correlacionando-os com a detecção de EBV. MATERIAIS E MÉTODOS: Foram obtidos 192 casos de adenocarcinomas gástricos de hospitais dos estados de São Paulo e do Ceará, dos quais 160 foram submetidos à técnica de RNA-hibridização in situ para detecção de EBV. RESULTADOS: Dos 160 casos, 11 (6,9 por cento) foram EBV-positivo, exibindo intensa marcação nuclear em células tumorais. Destes, dois casos também apresentaram linfócitos infiltrados marcados. Não encontramos marcação em tecido normal ou pré-neoplásico. São Paulo e Ceará apresentaram as frequências 3/60 (5 por cento) e 8/100 (8 por cento), respectivamente, e maior relação do EBV com indivíduos do sexo masculino, de idade avançada, com tumores do tipo intestinal, de estadiamento elevado e grau pouco a moderadamente diferenciado. Os casos do Ceará exibiram aumento relativo de tumores EBV(+) localizados na cárdia, enquanto os casos de São Paulo demonstraram aumento naqueles localizados no corpo gástrico. CONCLUSÃO: A frequência de tumores EBV(+) do presente estudo situa-se nos valores descritos na literatura mundial. Entre os achados, um deles não encontra paralelo na literatura mundial e refere-se ao elevado percentual de tumores EBV(+) no corpo gástrico observado nos casos de São Paulo.
INTRODUCTION: The Epstein-Barr virus (EBV) has been associated with approximately 10 percent of gastric adenocarcinomas, which represents more than 50,000 cases/year worldwide. Despite the studies undertaken in several countries, some clinical-pathological aspects remain contentious. OBJECTIVE: The objective of this study was to analyze clinical-pathological features of gastric adenocarcinomas from two Brazilian states, São Paulo and Ceará, by correlating them with EBV detection. MATERIALS AND METHODS: One hundred ninety-two gastric adenocarcinoma cases were selected from hospitals in São Paulo and Ceará, of which 160 were submitted to RNA in situ hybridization for EBV detection. RESULTS: Eleven (6.9 percent) out of 160 cases were EBV-positive with intense nuclear staining in tumor cells. Among these, two cases also showed stained infiltrating lymphocytes. There was no staining in normal or preneoplastic tissue. São Paulo and Ceará yielded the respective results: 3/60 (5 percent) and 8/100 (8 percent). In both states, EBV was more prevalent among elder male patients with little to moderately differentiated intestinal tumors in advanced stage. Ceará cases substantiated a relative increase in EBV(+) tumors located in the cardia, whereas São Paulo cases presented an increase in the gastric corpus. CONCLUSION: The frequency of EBV(+) tumors is similarly described in the literature. Among our findings, the elevated percentage of EBV(+) tumors in the gastric corpus, which was observed in São Paulo cases, is unprecedented in the literature.
ABSTRACT
We report on a neuropathological analysis of two cases of TSP/HAM originating from Brazil. These two cases had, respectively, an evolution of 13 and 40 years. The main neuropathological findings consisted of spinal cord atrophy, mainly the lower thoracic cord, diffuse degeneration of the white and grey matter, rare foci of mononuclear and perivascular cuffs, and hyaline hardening of arteriolae. The supraspinal structures were normal, excepting for a slight gliosis in the cerebellum. An analysis on the long evolutive cases as described in the literature is outlined in this study
Subject(s)
Humans , Female , Paraparesis, Tropical Spastic , Brain , Nerve Degeneration , Spinal CordABSTRACT
A síndrome de acardia fetal é uma rara complicaçao de gestaçoes gemelares monozigóticas, ocorrendo uma vez em cada 35.000 partos. O prognóstico é fatal para todos gêmeos acárdicos e para 50-75 por cento dos gêmeos normais. Neste trabalho é apresentado um caso de hemiacardia fetal (acardia parcial) em gravidez gemelar. O diagnóstico foi feito utilizando-se ultra-sonografia, ecocardiografia fetal e foi confirmado após o parto.
Subject(s)
Humans , Female , Pregnancy , Adult , Fetal Heart/abnormalities , Fetal Diseases , Prenatal Diagnosis , Twins, Monozygotic , Syndrome , Ultrasonography, PrenatalABSTRACT
Vinte e dois casos de LES acompanhados no Hospital das Clínicas da Universidade Federal do Ceará que foram submetidos à autópsia foram revisados, com ênfase para as anormalidades cardíacas. O material, incluído em parafina, de 20 desses casos foi processado para coloraçao pela hematoxilina- eosina e procedeu-se à reanálise de microscopia. Achados clínicos cardíacos fo ram relatados em 41 por cento dos casos, enquanto que foram encontradas altera çoes em 65 por cento dos casos disponíveis para análise histopatológica. Clinicamente, pericardite foi a anormalidade predominante (23 por cento). A autópsia, o pericárdio foi também o folheto mais freqüente envolvido (45,5 por cento), se guido pelo miocárdio (36,4 por cento) e endocárdio (22,7 por cento). Os achados clínicos e de autópsia assemelham-se aos dados de literatura. Os autores alertam para a importância da pesquisa de alteraçoes cardíacas no LES, uma vez que o reconhecimento precoce pode influir no prognóstico