ABSTRACT
No presente trabalho, são mostrados resultados de um estudo piloto direcionado à detecção de seqüências de enterovirus em tecido cardíaco obtido a partir de biópsias endomiocárdicas de pacientes com doenças cardíacas na região Amazônica. Seis amostras coletadas de três pacientes foram analisadas por RT-PCR obtendo-se três espécimes positivos e três negativos. Esses achados preliminares sugerem a participação dos enterovirus na etiologia de doenças cardíacas, principalmente miocardites, e justificam estudos mais amplos nesse assunto.
In the present report we describe the results from a pilot study aimed at detecting enterovirus sequence in cardiac tissues, obtained through endomyocardial biopsies, from patients suffering from cardiac diseases in the Amazon region. Six samples that were collected from three patients were analysed by RT-PCR showing 3 positive and 3 negative results. These preliminary findings suggest the participation of enteroviruses in the etiology of cardiac diseases, mainly myocarditis, and warrant further and broader local studies on this subject.
Subject(s)
Adult , Aged , Female , Humans , Male , Heart Diseases/virology , Heart/virology , Enterovirus/isolation & purification , Enterovirus Infections/complications , Myocardium/pathology , Brazil , Heart Diseases/etiology , Heart Diseases/pathology , DNA, Viral/analysis , Enterovirus/genetics , Enterovirus Infections/pathology , Polymerase Chain ReactionABSTRACT
We attempted to assess the role of enteroviruses in the etiology of myocarditis (MC), pericarditis (PC) and dilated cardiomyopathy (DCM) among 15 in-patients at a public hospital in Belém, Brazil, from November 1992 to December 1993. We obtained stool specimens and throat swabs from each patient (particularly acute cases) and, when possible, acute and convalescent serum samples for both isolation and serological procedures. MC, PC and DCM ocurred in 10, 2 and 3 patients, respectively, mostly in the 0- to 10- year age group. Neutralizing antibody seroconversions were detected as follows: one for Coxsackievirus (Cox) B2 in one patient suffering from MC, and two for Cox B4, in patients with DCM and MC. In addition, antibody titers of 1/320 against Cox B2 and Cox B4 were noted in two other patients, one suffering from PC and the other presenting MC. Isolation of echovirus (ECHO) serotype 1 was recorded ina a patient with MC, without either seroconversion or high antibody levels for Cox B 1 to 6. These results indicate that enteroviruses may be involved in the etiology of MC, PC and DCM in the Amazon region.
Subject(s)
Humans , Infant , Child, Preschool , Child , Adolescent , Adult , Middle Aged , Cardiomyopathy, Dilated/virology , Enterovirus/physiology , Myocarditis/virology , Pericarditis/virology , Brazil , Enterovirus/isolation & purification , Enterovirus/physiology , Feces/virology , In Situ Hybridization , Polymerase Chain ReactionABSTRACT
Os autores apresentam um caso de diverticulo de jejuno perfurado por espinha de peixe, nao constatando na literatura pertinente nenhum caso semelhante