ABSTRACT
OBJECTIVE@#To study virulence and regulatory genes (hlyA, ctxB, tcpI) in clinical strains of Vibrio cholerae (V. cholerae), simultaneously.@*METHODS@#Three important genes, tcpI, hlyA and ctxB were used for detection of toxigenic and pathogenic V. cholera by chain reaction assay method.@*RESULTS@#According to the results of the PCR, the incidence of hlyA, tcpI, and ctxB genes in clinical isolates was obtained as 94.7% (72 sample), 90.8% (69 sample), and 92.1% (70 sample), respectively. Five strains possessed all genes except ctxB, six strains possessed all genes except tcpI, four strains possessed all genes except hlyA, one strain possessed only hlyA and 60 strains contained a combination of three genes, Including hlyA, ctxB and tcpI.@*CONCLUSIONS@#Result show that this method could be reliable to detect toxigenic-pathogenic strains of V. cholerae in Iran.
Subject(s)
Humans , Bacterial Proteins , Genetics , Cholera , Microbiology , DNA, Bacterial , Genetics , Feces , Microbiology , Genes, Bacterial , Genetics , Hemolysin Proteins , Genetics , Iran , Multiplex Polymerase Chain Reaction , Methods , Repressor Proteins , Genetics , Sensitivity and Specificity , Vibrio cholerae , Classification , Genetics , Virulence , GeneticsABSTRACT
Considering the significant incidence of nosocomial infections in hospitalized patients, this study was done to determine the prevalence of Staphylococcus aureus strains isolated from wound infection and drug sensitivity pattern, Tehran-Iran. In this descriptive study, Staphylococcus aureus isolated and identified according to standard procedures from the wound infections of 614 patients referred to Baqiyatallah hospital, Tehran-Iran during 2006-07. The samples were examined and antibiogram was performed by disc diffusion method on Mueller Hinton agar with 12 antibiotics. 100 [16.28%] of wound infection of Staphylococcus aureus was isolated from 614 patients. The infection rate in men was twice compared to women. The highest rate 29 [29%] was observed in people aged 40 to 60 group. Also specimen's patients with immunosuppressive diseases [28 cases], surgical site infection [16 cases] and normal wounds [13 cases] were considered to be most prevalent isolates. Antibiotic sensitivity testing revealed that 96 [96%] of isolates were sensitive to vancomycin, 95 [95%] and 92 [92%] were resistant to penicillin and cotrimoxazole, respectively. Our result showed that 43% of strains were resistant at 11 antibiotics. This study showed that the prevalence of Staphylococcus aureus was 16.28% of samples, with 43% antibiotic resistance. The highest sensitivity was toward to vancomycin