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Aim To investigate the protective effect of 1, 8-Cineole on the injury of human aortic endothelial cells (HAECs) induced by high glucose (HG) via regulating autophagy. Methods Cells were incubated with different doses of 1, 8-Cineole followed by exposing to HG for 60 h, and MTT assay was used to analyse cell viability, lactate dehydrogenase (LDH) was used to detect cytotoxicity, and Western blot was used to detect Beclin1, LC3-II/I, p62, caspase-3 and caspase-9 expressions. Autophagy inhibitor (chloroquine, CQ) was treated on HAECs, and the expressions of Beclinl, LC3-II/I, p62, caspase-3 and caspase-9 were measured by Western blot. Results 1, 8-Cineole increased cell viability, reduced the content of LDH, activated autophagy and inhibited apoptosis. Compared with control group, the expression of Beclinl, LC3-II/I, p62, caspase-3 and caspase-9 in CQ group increased. Simultaneously, the expression of above-mentioned between CQ + HG group and CQ + HG + CH group. Conclusions 1, 8-Cineole has protective effect on the injury of HAECs induced by high glucose, and its effect is related to improving autophagy flux.
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Objective@#To explore the impact of procedural behavior management on children with dental fear (DF) using the interactive mode of coparticipative doctor-patient interactions.@*Methods@#Ninety-eight children with dental fear and aged 3-6 years were randomly divided into an observation group and a control group. Dental treatment was performed on the observation group under the coparticipation model, while the control group adopted the traditional tell-show-do (TSD) operation. The entire process of diagnosing and treating each child was recorded, and the degree of dental fear was assessed using a behavioral grading method as the standard.@*Results@#The degrees of fear in the children in the observation group and the control group were 3.571 ± 0.913 and 3.857 ± 1.000. The two groups showed no significant difference in the degree of fear (t=1.477, P > 0.05). During the treatment, the fear scores of the children in the observation group and the control group were 1.428 ± 1.061 and 3.286 ± 0.707. The two groups showed statistically significant differences in fear scores (t=10.198, P < 0.001).@*Conclusion @#In the coparticipative model, the fear level of DF children was significantly reduced by process-based behavior management, which helped to improve the dental fear of the children.
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<p><b>OBJECTIVE</b>To explore the effects of bisphenol A (BPA) exposure on toxicity characteristic and OCT4 and SOX2 gene expression of mouse embryonic stem cells (mESC).</p><p><b>METHODS</b>mESC were cultured, and treated with the doses of 10(-8), 10(-7), 10(-6), 10(-5), 10(-4) mol/L respectively of BPA and DMSO (the solvent control group)for 24 hours, and three groups of cells were treated with the same method. The morphological changes of mESC in the control and exposure groups were observed through an inverted microscope. Cell counting kit 8 (CCK8) was used to detect the effects of BPA on proliferation of mESC, and based on the results, the half inhibitory concentration (IC50) was calculated. Real-time fluorescent quantitative polymerase chain reaction (RT-QPCR) and western blotting were used to detect the expression of OCT4 and SOX2.</p><p><b>RESULTS</b>BPA had certain toxicity on mESC, the treatment of BPA significantly increased cell toxicity in a concentration-dependent manner, and the IC50 was 4.3×10(-4) mol/L, combined with the BPA exposure concentration of the environment and the related literature, eventually taking the five concentrations of 10(-8), 10(-7), 10(-6), 10(-5), 10(-4) mol/L as the experimental groups. The mESC morphology were effected after the treatment of BPA for 24 h, compared with the control group, the number of cells decreased, appearing some floating cells, and the cell cloning became irregular and differentiation in the higher concentration groups. The OCT4 mRNA expression level in the 10(-7) mol/L (1.146 ± 0.087), 10(-6) mol/L (1.156 ± 0.030), 10(-5) mol/L (1.158 ± 0.103) and the 10(-4) mol/L (1.374 ± 0.053) dose group were all significantly higher than the control group (1.000 ± 0.000) (t values were -2.384, -2.953, -3.203, -4.021 respectively, P value all < 0.05). Meanwhile, the SOX2 mRNA expression level in the 10(-4) mol/L (1.113 ± 0.052) were higher than the control group (1.000 ± 0.000) (t value was -2.765, P value < 0.05). Moreover, the OCT4 protein expression level in the 10(-5) mol/L (1.360 ± 0.168) and 10(-4) mol/L (1.602 ± 0.151) were all significantly higher than the control group (1.000 ± 0.000) (t values were -3.538, -4.002 respectively, P value all < 0.05), while no obvious change of the SOX2 protein expression level was detected in all treated groups.</p><p><b>CONCLUSION</b>BPA in a certain dose range could upregulate the expression of OCT4 gene in mouse embryonic stem cells while had no significant effect on the expression of SOX2 gene.</p>
Subject(s)
Animals , Mice , Benzhydryl Compounds , Toxicity , Cells, Cultured , Embryonic Stem Cells , Metabolism , Gene Expression , Octamer Transcription Factor-3 , Genetics , Phenols , Toxicity , SOXB1 Transcription Factors , Genetics , Signal TransductionABSTRACT
This research is to investigate and analyze the reading characteristics of students in medical college of Foshan Science and Tecnoology University during their clinical practice period,to understand the trainee's reading motive,their expectation of a reading teacher,and their demanding of serve from the college library,thus exploring the method and the countermeasures of the reading guides