ABSTRACT
Objective:To study the effects of different processes on diosgenin content in polygonatum from Shaanxi. Methods:The content of diosgenin was determined by HPLC on a column of Woburn C18 (250 mm × 4. 6 mm, 5 μm) with the mobile phase of acetonitrile-water(90 ∶10)at the flow rate of 1.0 ml·min-1, the detection wavelength was 203 nm, the column temperature was 30℃, and the injection volume was 30 μl. Results:The linear range of diosgenin was 0. 892-5. 352 μg(r=0. 999 9), and the aver-age recovery was 99. 5% (RSD=2. 53%). The content of diosgenin was significantly various among the samples of polygonatum with different processes from Shaanxi (P<0. 05), and that in the raw slices was the highest followed by the steam samples, while that in al-cohol evaporate slices was the lowest. Conclusion:The results of the research can provide reference for the quality control and process-ing methods of polygonatum from Shaanxi.
ABSTRACT
Objective: To study the change rule of polysaccharide content in different processed products of polygonatum from Shaanxi. Methods:The content of polysaccharides was determined by UV spectrophotometry at the wavelength of 580nm after colored by 0. 2% anthranone-sulfuric acid solution. Results: The content of polysaccharide was significantly different among different pro-cessed products of polygonatum, and that in the raw slices was the highest followed by alcohol evaporate slices, while that in the steamed samples was the lowest. Furthermore, the extract and the content in the different processed samples of polygonatum showed a similar regularity. Conclusion:The results of the research can provide reference for the quality control and processing methods of po-lygonatum slices from Shannxi.