in vitro comparative study of apical microleakage of biocalex and white MTA as root-end filling materials

For sealing a cavity prepared in endodontic surgery, there is a need for a material to obtain an apical seal and prevent bacterial and tissue exudates. The purpose of this study was to compare the sealing ability of Biocalex and white MTA as root-end fillings. In this in vitro experimental study, 36 single-rooted extracted human adult teeth with minimum root curvature were cleaned, shaped and obturated with lateral condensation technique, 3 mm of the root apices were resected and a 3 mm deep retrograde cavity was prepared. Teeth were assigned randomly into two groups of 15 teeth each and retrofilled with Biocalex and White MTA. Three teeth served as positive and three teeth served as negative controls. All root surfaces, except the ending part, were covered with two layers of nail polish. Then the roots were stored in 2% methylene blue for 72 hours and then embedded in polyester resin. Next, they were sectioned buccolingually by diamond bur and maximum extent of dye penetration was evaluated through stereomicroscope. Statistical analysis was carried out by student t-test. The level of significance was set at 95%. It was shown that retrofills with Biocalex had significantly less microleakage compared with white MTA. The findings of this study showed that retrograde filling with Biocafex can achieve proper sealing ability and can be used as an alternative for MTA [P<0.001]

histologic evaluation of rat connective tissue reaction to three different gutta-percha cones [ariadent, roeko and diadent]

Regarding the manufacture of different gutta-percha cones and the importance of apical seal in a successful root canal therapy, this experimental study was designed to compare the invivo effects of Ariadent gutta-percha with Roeko and Diadent. In this exprimental invivo study, 29 mature Albinus rats [mean weight 202/77 gr] were selected. Four intradermal incisions were made in the dorsal part of each rat. In three incised areas, a piece of each gutta-percha was placed and one of the four incised areas was assumed as the negative control. Animals were sacrificed at 15, 30 and 60 day intervals and then specimens were taken from subcutaneous connective tissues of rats. All of the specimens were prepared histologically and stained using H and E staining method, histological assessment was based on the number of inflammatory cells. Data were analysed using One-Way ANOVA and Duncan test with 95% confidence level through SPSS software. The results revealed that Ariadent gutta-percha in 15 day interval was more reaction inducing than Roeko and Diadem but in 30 and 60 day intervals, tissue biocompatibility of Ariadent was the same as Roeko and Diadent. Iranian Ariadent gutta-percha can be considered as a substitute for other foreign gutta-percha cones