ABSTRACT
Effect of snuff extract (SE) on cell proliferation as measured by 3H thymidine (TdR) uptake, induction of ornithine decarboxylase (ODC) and aryl hydrocarbon hydroxylase (AHH) was studied in primary embryonal mouse tongue cultures. Cultures treated with SE in combination with 7,12-dimethylbenz(a)anthracene (DMBA) showed inhibition of cell proliferation and decrease of ODC and AHH activities, compared to control, DMBA, and DMBA + 12-O-tetradecanoylphorbol 13-acetate treated cultures.
Subject(s)
Animals , Aryl Hydrocarbon Hydroxylases/antagonists & inhibitors , Carcinogens , Cell Division , Cells, Cultured , Mouth Neoplasms/etiology , Ornithine Decarboxylase/antagonists & inhibitors , Plants, Toxic , Tobacco, Smokeless/toxicity , Tongue/cytologyABSTRACT
The utility of hamster cheek pouch model for studies on oral carcinogenesis has been explored using 9,10-dimethyl-1-2-benzanthracene as a carcinogen. Based on the morphological, histopathological and electron microscopic observations the hamster cheek pouch carcinogenesis can be separated into different stages starting from the normal to the fully grown carcinomas. This system is reliable, precise, consistent and can be used for the evaluation of different agents for initiating or promoting effects and as well as for the studies on mechanism of oral carcinogenesis.