Detection of bcr-abl fusion gene mRNA level in K562/A02 cell line by real-time quantitative RT-PCR / 中国实验血液学杂志

This study was aimed to quantitatively analyze the mRNA level of bcr-abl fusion gene in K562/A02 cell line by real-time quantitative reverse transcriptase polymerase chain reaction (RQ-RT-PCR) technique. After being cultured for a period of time, the K562/A02 cell line was collected and RNA was extracted using TRIzoL kit. The real-time quantitative reverse transcriptase polymerase chain reaction technology was used to detect the level of bcr-abl fusion gene and internal reference abl gene. The results showed that a fine reproducibility was obtained between 10(7) and 10(3) copies/ml, reproducible sensitivity of RQ-RT-PCR was 10(-5). The expression of bcr-abl fusion gene in K562/A02 cells was higher and the level of bcr-abl mRNA was more than 100% in K562/A02 cells. It is concluded that RQ-RT-PCR is a reliable, sensitive and reproducible method for detecting mRNA level of bcr-abl fusion gene, which may be useful in monitoring the chronic myeloid leukemia.

Influence of magnetic Fe3O4 nanoparticle on functions of lymphocytes and macrophages in mice / 中国实验血液学杂志

This study was purposed to investigate the effects of magnetic nanoparticle of Fe3O4 (Fe3O4-MNPs) on murine immune system. ICR mice were assigned randomly into four groups which were treated with normal saline, low, middle and high dose of MNP-Fe3O4 respectively. The mice were killed after being exposed by intragastric administration for 2 weeks. The ratios of spleen weight to body weight, lymphocyte transformation rate in spleen suspension and phagocytic index of macrophage in abdominal cavity were detected. The results showed that the ratios of spleen weight to body weight in Fe3O4-MNP groups were not significantly different in comparison with the control (p > 0.05). The lymphocyte transformation rate in spleen suspension in Fe3O4-MNP groups were all higher than that in control group (-0.1775 +/- 0.0246), especially in the middle dose group (0.1833 +/- 0.0593) (p < 0.05), and the phagocytic index of macrophages in abdominal cavity of middle dose group (0.2051 +/- 0.0213) was higher than that of control group and other two Fe3O4-MNP group (low dose 0.1538 +/- 0.0100, high dose 0.1511 +/- 0.0184) (p < 0.05). It is concluded that suitable dose of Fe3O4-MNP can enhance the cellular immune activity and phagocytic function of macrophages of mice.

M-FISH technique in diagnosis and prognostic analysis for acute leukemia with complex chromosomal aberrations / 中国实验血液学杂志

The M-FISH includes multi-colour FISH and multiplex FISH, it represents one of the most significant developments in molecular cytogenetics of the past decade. This technique was originally designed to generate 24 colour karyotyping in human's 23 pair chromosome, now the technique has many variations and has been used in different fields. In leukaemia cytogenetics, the M-FISH now is used in detection for AL patients with following chromosome abnormality: (1) harbouring minimal chromosome translocation is respected; (2) chromosome translocation with complex abnormal karyotypes exists in patients with leukemia which are difficulty detected by using conventional method. The final results detected by M-FISH have guide significance for diagnosis, therapy and prognosis of AL patients. In this article the technical basis with commonly used probe for M-FISH, application of M-FISH in diagnosis, evaluation of therapeutic efficacy and prognostic analysis of AL patients are summarised.