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Objective@#To summarize the isolation characteristics and drug resistance of HACEK bacteria in lower respiratory tract infection of coal worker's pneumoconiosis.@*Methods@#From 2016 to 2018, K-B method and MIC method were used to test the susceptibility of 10 commonly used antimicrobial agents to isolated 46 strains of HACEK bacteria, and the drug resistance of lactamase and non-enzyme producing haemophilus was compared.@*Results@#Haemophilus influenza (Hi) accounted for 71.74% (33/46) of the HACEK bacteria in the lower respiratory tract infection patients of coal workers' pneumoconiosis in our hospital, Haemophilus parainfluenzae (HPi) accounted for 23.91% (11/46) , which corroded one strain of Eikenella and one strain of actinomycetes. HACEK bacteria were isolated most in winter and spring, and less in summer and autumn. Cefuroxime, ceftriaxone, amoxicillin/clavulanic acid and levofloxacin have good antimicrobial activity against HACEK bacteria. The sensitivity of non-enzyme-producing strains Tetracycline (TE) , Compound trimethoprim (SXT) , Azithromycin (AZI) and Ampicillin (AMP) was stronger than that of enzyme-producing strains (P<0.05) .@*Conclusion@#Hi is the main HACEK bacteria isolated from patients with lower respiratory tract infection of coal worker's pneumoconiosis, followed by Hpi. The enzyme-producing strains of these bacteria are resistant to AMP, SXT and AZI to some extent. These drugs should not be the first choice for treatment.
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Objectives:To observe growth suppressive effects of human wild type p53(wt p53) gene on human esophageal cancer cell line. Methods:Using the retroviral vector to introduce exogenous wt p53 gene into human esophageal cancer cell line ECA109,the gene expression and tumor inhibition were studied in vitro and in vivo . Results:The expression of p53 in transfected cell lines(ECA 109/p53 ) was increased.The growth rates and the ability to form colony in soft agar were greatly inhibited in ECA 109/p53 cells versus ECA109/neo and ECA109 cells.The G 0+G 1 ratio increased and S ratio decreased in cell cycle distribution,and apoptosis index significantly rose in the ECA109/p53 cells,which were confirmed by FCM analyzing.The tumorigen icity of ECA109/p53 cells in nude mice was obviously suppressed by exogenous p53. Conclusions:Exogenous wt p53 mediated by retroviral vector could inhibit the growth of human esophageal cancer cell.
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Objective To investigate the regulation of metabolic characteristics of metronidazole and tinidazole in human bile,and provide a theoretic basis for physician selecting antibiotic rationally when biliary tract was infected.Methods Samples of hman bile were obtained by ERCP plus ENBD,contents of metronidazole and tinidazole were simultaneously measured by HPLC method in the human bile and plasma.Results 0 25 hour after metronidazole and tinidazole were given through iv,contents of two antibiotics had reached the effective bactericida.Concentraction,content of tinidazole in the bile was relatively stable,but its C max was lower as compared with the metronidazole,contents of the two antibiotics in bile were continuously higher than those of the effective bactericidal in 24 hours,and after 16 hours and re-raise was observed in contents of the two antibiotics in bile.Conclusion As maintaining a higher concentration level for long time in the bile by iv,metronidazole and tinidazole were recommended in the patients with an infection in biliary truct.
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Objective To explore curative effect of intraperitoneal chemotherapy to treat patients with tension ascites caused by advanced stage of liver cancer. Methods 61 patients with tension ascites caused by advanced stage of liver cancer were divided randomly into three groups, patients in group A (n=26) were treated by ultrafiltration ascites and peritoneal reinfusion (A-P) combined with intraperitoneal chemotherapy; patients in B group (n=18) were treated by paracentesis and intravenous albumin infusion combined with intraperitoneal chemotherapy; patients in group C (n=17) were treated by paracentesis and intravenous albumin infusion. Clinical situation, biochemical alteration, sodium metabolism and RAAS metabolism were observed before and after treatment.Results The findings revealed that definite clinical amelioration in all patients. Patients in three groups, biochemical examinations were improved during 24h after treatment, including of endogenous creatinine clearance rate and urine sodium output. But in group B and C, the biochemical examinations returned to the former levels and the levels of serum albumin was markedly reduced during 72h after treatment. The short-term effective rate on postoperative ascites in group A, B, C were 57.7%, 44.4% and 11.9% respectively.Conclusion A-P associated with intraperitoneal chemotherapy is an effective and safe therapy for patients with tension ascites caused by advanced stage of liver cancer.
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Objective To compare the expression level of calpain and calpastatin mRNA in rheumatoid arthritis (RA) and osteoarthritis (OA) synovial tissues (ST) and analyze the antigen epitopes of human calpastatin recognized by RA sera.Methods Total RNA of three RA and two OA patients was isolated from ST and expression levels of calpain and calpastatin mRNA were detected by reverse transcription polymerase chain reaction (RT PCR).According to hydrophilicity of human calpastatin (Accession,U 31346), overlapping oligopeptides were synthesized by auto spot Robot in N terminal (L domain) and C terminal (Ⅳ domain) of calpastatin.The epitopes of synthesized peptides were analyzed by RA sera using dot ELISA.Results mRNA expression of calpain showed higher than that of calpastatin in both OA and RA.Meanwhile,the mRNA expression level of calpain increased in RA more than that in OA.Three antigen epitopes of human calpastatin (with the sequence DKDLDDALD,DTIPPEYRH and QDPIDALSG) were identified by RA sera,while control sera failed to react with synthetic peptides.Conclusion The calpain calpastatin system may participate in pathogenic mechanism of RA,and the calpastatin may be a target for autoantibody of RA,which implies that it is not only possible to treat RA patients with synthetic peptide of human calpastatin,as a chemotherapeutant,but also a materials for immunodiagnosis of RA.
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Objective: To investigate the effect of recombinant human growth hormone(rhGH) in the treatment of liver cirrhosis with hypoproteinemia. Methods: 28 patients with liver cirrhosis,whose albumin level was below 35 g/L,were randomly divided into two groups:rhGH treatment group and control group.Serum albumin,ALT and other items were measured 30,60 and 90 days after the start of treatment. Results: After 30 days,an increase in plasma albumin was observed in the two groups,but the change in the treatment group was more obvious than that in control group,An increase in plasma protein in treatment group and the decrease in control group were observed with significant difference between groups. Conclusion: Recombinant human growth hormone can increase the level of ambumin in liver cirrhosis patients and improve the function of liver.
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OBJECTIVE:To evaluate the3pharmacotherapeutic schemes for biliary tract infections by pharmacoeconomic method.METHODS:Scheme A:cefazolin+metronidazole;Scheme B:ampicillin+gentamicin+metronidazole;Scheme C:lome?floxacin injection+metronidazole.These three schemes were retrospectively analysed with cost-effectiveness analy?sis.RESULTS:Scheme C had the lowest cost-effectiveness ratio with the highest total effective rate.CONCLUSION:Scheme C is the best one.