ABSTRACT
Myeloid-derived suppressor cells(MDSC)are heterogeneous cells with the myeloid progenitor cells and the imma-ture myeloid cells accumulated in pathological conditions.MDSC can inhibit the host immune response to tumors via inhibiting the pro-liferation and cytotoxicity of T cells as well as promoting the proliferation of protumorigenic T regulatory cells(Treg).In addition,MD-SC can also promote the angiogenesis and the tumor invasion and metastasis.Therefore,MDSC are potential therapeutic targets for a variety of tumors.This review summarizes the biological function of MDSC along with the MDSC-targeted inhibitors and their applica-tions in cancer immunotherapy.
ABSTRACT
After treating with chemotherapy or immunosuppressant, malignant diseases of hematopoietic system such as leukemia, malignant lymphoma and aplastic anemia usually induced severe infection such as sepsis. Sepsis which is hard to be diagnosed causes high death rate. This study was purposed to establish an experimental sepsis mouse model so as to provide a basis for pathogenesis and intervention study. A classic caecal ligation and puncture (CLP) was used to establish experimental sepsis model. ELISA was used to detect levels of C5a, IL-6, TNFalpha, and IFN-gamma. Flow Cytometry was applied to measure apoptosis of lymphocytes in thymus and mesentery. The pathologic changes of thymus and spleen were confirmed by HE staining. The results showed that almost 70%-80% mice died at 72 hours after CLP. Only approximate 20% animal survived during finite time, mice in CLP group had significant weight lose. Meanwhile large release of different inflammatory mediators which are related with sepsis (C5a, IL-6, TNF-alpha, and IFN-gamma) was observed after CLP. Apoptosis of lymphocytes in thymus and mesentery lymphonodus was enhanced markedly after CLP. Significantly pathologic injury was also observed in thymus and spleen. It is concluded that a mouse model of experimental sepsis was successfully established by caecal ligation and puncture which can well mimic the clinical symptom of sepsis. The experimental sepsis mouse model provides an excellent tool for exploring the pathogenesis and intervention ways for sepsis accompanied with complicated malignant hematological diseases in vivo.