ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of kappa-opioid receptor stimulation on high glucose induced myocardial hypertrophy of neonatal rats.</p><p><b>METHODS</b>Using cultured myocardial cells as a model, the protein content was assayed with Lowrys method. The cardiomyocytes volumes were measured by computer photograph analysis system. The level of p-ERK44/42 was determined by Western blot.</p><p><b>RESULTS</b>Compared with the control, U50488H significantly inhibited the protein content and volumes of cultured hypertrophic myocardial cells induced by high glucose. Meanwhile the role of ERK was important.</p><p><b>CONCLUSION</b>The stimulation of kappa-opioid can inhibit myocardial hypertrophy induced by high glucose, which is possibly via attenuating p-ERK.</p>
Subject(s)
Animals , Female , Male , Rats , 3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer , Pharmacology , Animals, Newborn , Cardiomegaly , Metabolism , Cells, Cultured , Glucose , MAP Kinase Signaling System , Myocytes, Cardiac , Rats, Sprague-Dawley , Receptors, Opioid, kappa , MetabolismABSTRACT
Objective To demonstrate the inhibitory effect of adenosine A_1 receptor agonist R(-)-N6-(2-phe- nylisopropyl) adenosine (R-HA) and cross-talk between adenosine A_1 receptor and CaMKII on Isoproterenol (Iso)- induced hypertrophy in cultured myocardial cells in neonatal rats.Methods The protein synthesis was determined by incorporation of [~3H]-leucine into myocyte protein.The expression of CaMK Ⅱ ? B was determined by Western- blot.The [Ca~(2+)]i transient was measured in myocytes loaded with fura-2 by the spectrofluorometric method. Results R-PIA (1?mol/L) inhibited Iso(10 ?mol/L)-induced increase of [~3 H-]-leucine incorporation [(R-PIA: 974.8?58.6) vs (Iso:1220.8?240.5) count per min per well,P
ABSTRACT
The source of measure error and how to control the quality, reduce error, increase examination quality are described in the paper in clinical electrolyte analysis and measurements. The related factors include patient readiness, sample collection and preparation, condition of the instrument and reagents, data management, result report and so on.