ABSTRACT
Objective To elucidate the inhibitory effect of 131I-fulvestrant on the growth of human breast cancer cells and the effect on the important organs.Methods MTT assay was used to clarify the difference in killing effects of the 131I-fulvestranton on MCF-7 cells and MDA-MB-231 cells.Breast cancer MCF-7 cell xenografts in nude mice was establishied,and two different administration methods of the 131I-fulvestrant in the MCF-7 cell to nude mice were given respectively.Organs and tumours of nude mice were observed.Results MTT assay demonstrated that 131I-fulvestrant had similar cytotoxicity against MCF-7 cells and MDA-MB-231 cells,and the former was slightly stronger.Transient contact experiments showed that 131I-fulvestrant could play a tumor suppressor effect on MCF-7 cells continually,but MDA-MB-231 cells wasn't.After the injection of 131I-fulvestrant via caudal vein,the radioactivity concentration on tumor site accounted for (4.33 ± 0.28)％ of the total injection,and the volume of the tumor reduced before gradually increasing again.Radioactivity in the blood accounted for (20.76 ± 2.54)％ of the total injection.Qrgans like liver and kidney also showed radioaction distribution.Its distribution was accorded with the distribution of estrogen receptor.Local injection of 131I-fulvestrant got powerful killing effect on the tumor,and the distribution of the radioaction was mainly confined within the tumor.Conclusion 131I-fulvestrant has a good inhibitory effect on MCF-7 breast cancer cells,which is a superposition of radiotherapy and endocrine therapy,and it is controllable on the general condition and important organs of nude mice.
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ObjectiveTo evaluate the effectiveness of PBL teaching model for medical students in surgery bed side teaching.MethodsThe method of meta-analysis.ResultsFinally,twenty-six documents met the requirement.The resuhs of Meta-analysis showed that,in the aspect of theory improvement for medical students,WMD=6.46,95 ％ CI 为 2.56 ～ 10.36,P＜0.01 ; in the aspect of skill ability improvement,WMD=5.32,95 ％ CI 为 2.47 ～ 8.16,P ＜0.01.ConclusionThe model of PBL teaching could significantly improve the effectiveness in theoretical knowledge and skill ability.
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Objective To dectect the expression of CD54 in thyroid tissue of Hashimotos thyroiditis patients,and expression changes of CD54 on thyroid cells interferred by different agents.Methods The thyroid tissues from 41 cases of Hashimotos thyroiditis were collected and 26 normal thyroid tissues served as normal controls.All thyroid tissues were identified by pathological examination.The positive expression rate and area of CD54 were investigated by immunohistochemical method and quantitative analysis of image analysis system in all thyroid tissues.The expression changes of CD54 in the isolated thyroid cells interferred by 100,500,1 000 pg/ml IL-1? or 10 mg/L NaI or 1 000 pg/ml IL-1? and 10 mg/L NaI for 48 h were detected by flow cytometry.Results The positive expression rate of CD54 in Hashimotos thyroiditis tissues was much more than that of control tissues(P
ABSTRACT
Objective:To explore the difference between the growth inhibition effect of arsenic trioxide(As_2O_3)on the human breast cancer MCF-7 and multidrug resistance MCF-7/ADR cell line.Methods:MCF-7 and MCF-7/ADR cell was cultured with 0.5?mol/L～16?mol/L arsenic trioxide in vitro.MTT test was adopted to detect the growth suppression effect of As_2O_3 in the two cell lines.Apoptosis morphology was studied with light microscope and electron microscope.Apoptosis ratio was analysed with flow cytometry.Results:As_2O_3 could inhibit the proliferation of the MCF-7 and MCF-7/ADR cell line,especially that of 2?mol/L～16?mol/L,and the effect was dose-and-time dependent.The IC_(50) at 96h was 3.0?mol/L for MCF-7 and 12.8?mol/L for MCF-7/ADR.Cell morphology and flow cytometry revealed the growth suppression was caused mainly by apoptosis rather than necrosis;however,to obtain the same inhibitory level,MCF-7/ADR cell required higher concentration of As2O3 and longer processing time.Conclusion:As_2O_3 can inhibit the proliferation in MCF-7/ADR cell as well as MCF-7 cell,though MCF-7/ADR cell is tolerant to As_2O_3,but the tolerance is at a low level.