ABSTRACT
Objective:To establish a quantitative determination method for Pb , As, Cu, Cd and Hg in Fufang Danshen tablets by inductively coupled plasma mass spectrometry ( ICP-MS) with microwave digestion .Methods: The samples were digested by micro-wave and calibrated by the internal standard elements ( In, Ge and Bi ) , and with spinach as the controlled reference standard , the contents of Pb, As, Cu, Cd and Hg were analyzed.Results:The minimum detection limit of Pb, As, Cu, Cd and Hg was 6.5,2.3, 5.9,4.7 and 0.91 ng· g-1, respectively, a good linear relationship was obtained respectively within the concentration range (r>0.998 0) with the average recovery of 102.8%,105.1%,104.5%,106.8%and 92.4%and RSD of 2.1%,2.8%,1.9%,2.2%and 3.4%(n=9), respectively.Conclusion:The method is accurate and fast with little interference , and suitable for the determination of heavy metal elements in Fufang Danshen tablets .
ABSTRACT
Objective:To establish an HPLC method for the simultaneous determination of adenosine, schisandrin, schisantherin,deoxyschizandrin and schisandrin B in Hugan tablets. Methods:An Agilent Eclipse XDB column(250 mm × 4. 6 mm,5 μm)was used with the mobile phase of acetonitrile- 0. 7% phosphoric acid solution with gradient elution. The flow rate was 0. 80 ml·min -1 . The detection wavelength was set at 260 nm in 0-14 min and 250 nm in 14-58 min,the column temperature was 30℃ ,and the sample size was 10 μl. Results:There was a good linear relationship within the range of 2. 35- 47. 00 μg· ml -1(r = 0. 999 8)for adenosine,14. 90-297. 00 μg·ml -1(r = 1. 000 0)for schisandrin,3. 46- 69. 20 μg·ml-1(r = 0. 999 9)for schisantherin,4. 00- 80. 10 μg·ml -1(r = 1. 000 0)for deoxyschizandrin and 3. 42- 68. 30 μg·ml-1(r = 0. 999 9)for schisandrin B. The average recoveries for the five components were all above 98% . Conclusion:The method is simple and accurate with good reproducibility,which can be used for the determination of adenosine,schisandrin,schisantherin,deoxyschizandrin and schisandrin B in Hugan tablets.
ABSTRACT
Objective:To evaluate the protective effect of NAC on the acute liver injury of rat and its mechanism.Methods:36 Sprague-Dawley (SD) rats were randomly divided into saline control group (group A,n=12), acute liver injury group (group B,n=12) and NAC intervention group (group C,n=12).In group B&C, SD rats were injected intraperitoneally with D-galactopyranoside(D-gal) (1g/kg). In group C, SD rats were received N-acetylcysteine (NAC) three times after D-gal were given.Animals were killed at 24 hours after the D-gal injection. The pathologic section of rat liver were observed and the concentration of NO and the activity of NOS in rat liver tissue of each group were assayed.Results:The pathologic changes of rat liver were significantly improved in NAC group. The hepatic NO concentration of acute liver injury rats were significantly decreased in the NAC group.Whereas the serum NO concentration was significantly increased in the same group.It showed that the iNOS were inhibited but the cNOS were activated after NAC treatment.Conclusion:NAC can improve the liver tissue pathologic changes of D-gal acute liver injury in rats. The protective activities may associate with the influence on the different types of NOS activities and then change the NO level in different organs in rats.