Advance in study on 16S rRNA gene sequencing technology in oral microbial diversity / 中南大学学报(医学版)

The 16S rRNA gene is the most commonly used molecular marker for identifying microorganisms. It is used in sequencing technology, including the first-generation, the second-generation, and the third-generation sequencing technology. A large number of studies on the 16S rRNA gene have contributed to a deeper understanding of oral microbial diversity. In the healthy oral cavity, there is microbial diversity in time and space. With the occurrence or development of oral diseases such as caries, periodontal disease, or halitosis, the microbial diversity will be changed.

Correlation between children's dental decay and the contents of saliva CCL28 and secretory immunoglobulin A / 中南大学学报(医学版)

OBJECTIVE@#To explore the association of the dental decay of children with the contents of chemokine CCL28 and secretory immunoglobulin A (sIgA) in saliva.@*METHODS@#A total of 108 children in 2 kindergartens of Changsha, with age from 3 to 5 years old, were enrolled for this study. The saliva was collected from these children when they were in the examination of mouth. Th e children were divided into 3 groups: A non-caries group [dynamical mean-field theory (DMFT)=0], a low caries group (DMFT=1-4) and a high caries group (DMFT ≥ 5). Th e contents of CCL28 and sIgA were measured by ELISA.@*RESULTS@#The contents of CCL28 and sIgA in saliva were (121.22 ± 32.63) pg/mL and (16.49 ± 8.02) μg/mL, respectively. A positive linear correlation was found between the CCL28 content and sIgA content in saliva (r=0.734). Th e CCL28 and sIgA contents in saliva were positively correlated with the degree of dental caries in children (P<0.05).@*CONCLUSION@#The dental decay of children leads to the secretion of chemokine CCL28, which promotes the secretion of sIgA in saliva.

Changes of caries activity and calcium, phosphorus concentrations in saliva of patients with nasopharyngeal carcinoma before and after radiotherapy / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To analyze caries activity of nasopharyngeal carcinoma (NPC) patients before and after radiation therapy. The study was also designed to analyze calcium and phosphorus concentrations in saliva of NPC patients before and after radiotherapy.</p><p><b>METHODS</b>Twenty-eight NPC patients and 20 healthy controls were included in this study. Resazurin disc method was used to detect caries activity of NPC patients before and after 70 Gy radiotherapy. The atomic absorption spectrometry and molybdenum-stibium antispetrophotography method were used to analyze calcium and phosphorus concentrations in saliva of NPC patients before and after 70 Gy radiotherapy and the healthy controls.</p><p><b>RESULTS</b>The results of resazurin disc method showed caries activity of NPC patients was increased after radiotherapy (P < 0.01). Calcium concentration in saliva of NPC patients before radiotherapy was (63.19 +/- 3.27) mg x L(-1), phosphorus concentration was (132.96 +/- 5.13) mg x L(-1). After 70 Gy radiotherapy, calcium concentration was (33.38 +/- 0.32) mg x L(-1), phosphorus concentration was (49.18 +/- 2.66) mg x L(-1). The difference of calcium and phosphorus concentrations in saliva before and after radiotherapy was significant (P < 0.01).</p><p><b>CONCLUSION</b>Caries activities of NPC patients increase after radiotherapy. Calcium and phosphorus concentrations in saliva decrease after radiation therapy.</p>

Expression and distribution of transforming growth factor β3 in the mouse tooth germ during development after advanced bell stage / 中南大学学报(医学版)

OBJECTIVE@#To observe the expression and distribution of transforming growth factor β3 (TGF-β3) in the mouse tooth germ after advanced bell stage, and to discuss the role of TGF-β3 during the development of tooth germs.@*METHODS@#BALB/C's mouse tooth germs at 4, 11, and 18 days postnatal (4dpn,11dpn,and 18dpn) were collected and processed for routine fixation, decalcification, embedding, and slicing. The expression of TGF-β3 was detected by immunohistochemisty.@*RESULTS@#As to 4dpn tooth germ: Positive expression of TGF-β3 was found in enameloblasts, odontoblasts, ambitus of dental pupilla, with weak positive expression in the intermedial of dental papilla. As to 11dpn tooth germ: Positive expression was seen in enameloblasts, with negative expression in odontoblasts and dental papilla. As to 18dpn tooth, positive expression of TGF-β3 was showed in the vessel wall and its surrounding, with negative expression in other areas.@*CONCLUSION@#The distribution of TGF-β3 expression showed a time-space characteristic during the mouse tooth germ development after advanced bell stage, which may exert a regulatory effect on tooth development and this effect is gradually getting weak with the development of tooth germs.

Therapeutic effect of the combination of traditional Chinese medicine and western medicine on patients with oral submucous fibrosis / 中南大学学报(医学版)

OBJECTIVE@#To observe the therapeutic effect of salvia miltiorrhiza and prednisolone on patients with oral submucous fibrosis (OSF).@*METHODS@#A total of 60 medium-term OSF patients and 60 advanced stage OSF patients were randomly divided into the first group (treated with both salvia miltiorrhiza and prednisolone) and the second group (treated with prednisolone alone). The clinical effect was compared between each group after 3-month treatment.@*RESULTS@#Difference was found in the lesion area of the medium-term cases and the advanced stage cases of the first group before the treatment [(10.37+/-3.40) cm2, (19.60+/-3.27) cm2] and after the treatment [(5.90+/-4.10) cm2, (16.33+/-4.02) cm2] (P0.05). There was difference in the therapeutic efficacy between the first group (70%) and the second group (16.67%) of the advanced stage cases (P0.05). The side effect of prednisolone could be reduced while used together with salvia miltiorrhiza.@*CONCLUSION@#There is obvious advantage in treating OSF by the combination of salvia miltiorrhiza and prednisolone.

Dentification ability of inbred strain mice tooth germs homologically transplanted into oral submucosa / 中南大学学报(医学版)

OBJECTIVE@#To establish a suitable environment for the bioengineered teeth in vivo by observing the dentification ability of BALB/C mice tooth germs homologically implanted into the oral submucosa.@*METHODS@#The first molar tooth germs of BALB/C mice 4 days after birth were transplanted into the oral submucosa of BALB/C male mice, and then recycled for regular histological observation after 1, 2, 3, and 6 week transplantation.@*RESULTS@#The tooth germs in the oral submucosa grew well with continuing developing enamelum and pulpodentinal complex, and the dentinal tubules were clear.@*CONCLUSION@#The environment of the BALB/C male mice oral submucosa is favorable for the growth of tooth germs in inbred strain BALB/C mice, and it can provide a new environment for the development of bioengineered teeth in vivo.

Epidemiological investigation on deciduous dental caries among children aged 2 approximately 4 years of Kaifu district in Changsha city / 中南大学学报(医学版)

OBJECTIVE@#To investigate dental caries of deciduous teeth among preschool children of Kaifu district in Changsha.@*METHODS@#According to the criteria recommended by the Third National Oral Health Investigation, deciduous caries of 459 children aged 2 to 4 were clinically examined. The mean decayed missing filled teeth (dmft) and the prevalence of dental caries for each age group were calculated. The Results were statistically analyzed using SPSS13.0 software package.@*RESULTS@#The prevalence of dental caries was 39.65%.The mean dmft score was 1.32. The prevalence of dental caries and mean dmft increased as children grew up (P<0.05).Dental caries of maxillary deciduous central incisor and mandibular second deciduous molar were significantly more common than other teeth. The filling rate was 7.57%, which increased with age. The prevalence of early childhood caries was 17.21% (17.90% for males and 16.34% for females). CONCLUSION Deciduous dental caries of preschool children are an important problem, and the filling rate is very low. Necessary measures for early prevention of dental caries must be taken.

Emphasis on the Construction of the Stomatology Experimental Teaching and the Cultivation of Students' Innovative Ideology / 中华医学教育探索杂志

In order to strengthen the stomatology experiment teaching,mehods such as constructing the stomatology experimental teaching platform,improving teaching methods and reforming the system of exam and assessment were adopted.Through these measures,opration and the innovative ideology of students were obviousely enhanced,students' interest were estimulated,the innovative spirit was enlightened and the comprehensive potential ability was developed.

Effects of tumor necrosis factor-? and interferon-? on proliferation of oral submucous fibrosis-derived fibroblasts / 实用口腔医学杂志

Objection: To investigate the effects of tumor necrosis factor-?(TNF-?)and interferon-?(IFN-?) on the proliferation of fibrobl asts from healthy persons(NM-FB) and patients with oral submucous fibrosis(OSF -FB).Methods: Fibroblast cultures were established from t he buccal mucosa of five healthy individuals and six patients with OSF. The effe cts of TNF-? and IFN-? on the proliferation of NM-FB and OSF-FB were studi ed by MTT colorimetric assay.Results: TNF-? at 100～10 0 00 U/ml stimulated NM-FB and OSF-FB proliferation (P