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This article reported a rare case of harlequin ichthyosis which was indicated with multiple structural abnormalities by prenatal ultrasound and diagnosed by trio-based whole-exome sequencing (Trio-WES). Prenatal diagnosis was performed because the ultrasound at 24 +4 gestational weeks revealed the fetus presenting with eclabium, flattened nose, short mandible, small auricle and abnormal posture of the toes. Copy number variation sequencing (CNV-seq) showed no chromosome aneuploidy or pathogenic copy number variants over 100 kb in the fetal or parental samples. Trio-WES showed that the fetus carried two heterozygous mutations, c.2593-1G>A and c.7444C>T in ABCA12. Sanger sequencing confirmed that c.2593-1G>A, a previously unreported variant, was paternally inherited and c.7444C>T was maternally inherited. Both parents had normal phenotype. The fetus was finally diagnosed with harlequin ichthyosis. After prenatal counseling, the parents made an informed choice to terminate the pregnancy at 28 +4 gestational weeks. The stillborn fetus showed multiple malformations The variants in this case expand the spectrum of variants in ABCA12 gene.
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This paper focuses on the practical necessity of discipline-integrated PBL curriculum in cultivating clinical thinking ability of college students majoring in clinical medicine. Through the teaching process of group discussion of some real and complete cases, this paper explains in detail how to cultivate medical students' clinical thinking ability by discipline-integrated PBL curriculum, discusses the implementation of ideological and political education associated with clinical medicine by heuristic teaching from close touching with clinical case and implicitly infiltration of the socialist core values such as dedication and integrity, and elaborates the humanistic quality and psychological comfort levels of medical students by being close to clinical patients. After five years of teaching practice, the teaching effect of discipline-integrated PBL curriculum has been approved by the national clinical medicine professional certification experts and praised by students. We believes that the development of discipline integrated PBL curriculum in medical college can strengthen students' problem-based autonomous learning ability, significantly improve the two-way integration ability between basic medical courses and basic medicine, and significantly build students' clinical thinking and clinical decision-making ability.
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To explore the development tendency and major influential factors of traditional Korean medicine (TKM) resource and service by statistics analysis. Data are mainly from the Yearbook of Traditional Korean Medicine (2009-2015). Ministry of Health and Welfare Statistical Yearbook 2016, and the Ministry of health and welfare practicing qualification management system. This article analyzed the related indicators, such as the average annual growth rates and the proportion in the national medical system. From 2006 to 2015, the average annual growth rate of the number of TKM hospital and TKM clinics were 7.0％ and 3.2％ separately; the average annual growth rate of TKM doctor and TKM pharmacists were 4.3％ and 10.0％ separately; the average annual growth rate of the number of beds in TKM institutions were 9.3％; and the average annual growth rate of the number of applications for outpatient and inpatient reimbursement for medical insurance of TKM institutions were 12.3％ and 20.5％ separately. From 2006 to 2015, the resources and services of TKM presented an increasing tendency, and the accessibility of TKM resource and service was enhanced. It is suggested that China should use reference from the aspects of strengthening policy, expanding the sources of capital investment, and expanding the coverage of medical insurance.
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Objective To grasp the current situation and trend of health personnel development in public TCM hospitals through the analysis of health personnel statistics data of public TCM hospitals in China; To provide decision-making references for management departments. Methods Data were from the National Health and Family Planning Commission Statistical Information Center. This article analyzed staff number, the average speed of development, the average annual growth rate, health staff age, academic qualifications and job titles and other indicators of TCM public hospitals during 2010-2015. Results From 2010 to 2015, there was an annual increasing tendency of the number of TCM health personnel, and the average annual growth rate was 5.9％, but the growth of the percentage of the TCM health personnel to the total health personnel was only 0.3％. In 2015, the proportion of TCM health personnel was less than 50％; the doctor-to-nurse ratio was 1:1.18; the educational level of postgraduate and undergraduate were 14.0％ and 48.0％ respectively, less than the average level of national hospitals. Conclusion From 2010 to 2015, there is an increasing tendency of the TCM health personnel of public TCM hospitals, but the proportion of TCM health personnel is low. The doctor-to-nurse ratio is reasonable gradually, but there is still a gap compared with the average level of the national hospitals. The average age of technical health personnel tends to be younger. The proportion of high educational level health personnel is increasing, but the proportion of senior technical position is reduced. The management departments should take appropriate measures to promote the rational allocation of human resources and development of TCM hospitals.
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Objective:To investigate the effects of ALEX1 overexpression on cell proliferation and apoptosis of human breast cancer cell line MCF-7.Methods: MCF-7 cells were infected recombinant lentivirus LV5-ALEX1 and the negative control lentivirus LV5-NC,respectively.After 72 h, the expression of ALEX1 was detected by Real-Time PCR and Western blot.CCK8 assay were performed to observe the proliferation ability after 24 h, 48 h, 72 h, 96 h.The effect of overexpression ALEX1 on apoptosis was determined by flow cytometry.The level of Bax,BCL-2 and active caspase3 was detected by Western blot.Results:The mRNA level of ALEX1 markedly increased after 72 h(165.81±12.14 vs 52.29±2.32,P<0.01).In CCK8 assay,the results revealed that the cell pro-liferation was inhibited compared with control group in 48 h,72 h,96 h( P<0.05).The results revealed that overexpression of ALEX1 enhanced MCF-7 apoptosis(20.55%±2.50 % vs 3.60%±1.614%,P<0.05).The results by Western blot showed that the protein levels of Bax and active caspase were increased in LV5-ALEX1 group compared with LV5-NC group.However,the protein levels of BCL-2 was decreased in LV5-ALEX1 group compared with LV5-NC group.Conclusion:Overexpression of ALEX1 significantly reduced MCF-7 cancer cells proliferation and induced MCF-7 cells apoptosis.
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Objective:To investigate the inhibitory effect of dihydroartemisinin on non-small cell lung cancer H1299 cells.Methods:Through the CCK-8 method for determining the IC 10 of dihydroartemisinin ,choose low dose IC 10 as the experimental concentration,CCK-8 to observe artemisinin in low doses of H1299 cell proliferation, cycle and the influence of radiation sensitivity.Results:IC10 of dihydroartemisinin was 14.87 μmol/L,dihydroartemisinin could inhibit proliferation of H 1299 cells,slow down the cell cycle , and increased the radiation sensitivity.Conclusion: Dihydroartemisinin can inhibit cell proliferation , cell cycle arrest in S phase ,increase the radiation sensitivity.
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Aim To investigate the effect of small inter-ference RNA-mediated silcencing of the Bmi-1 gene on cell invasion and metastasis in human nasopharyngeal carcinoma cell line CNE-1 . Methods Chemically syn-thesized siRNA targeting the Bmi-1 gene was transfect-ed into CNE-1 cells, which had high invasive and me-tastatic potential. The expression of Bmi-1 mRNA and protein were detected by quantative Real-time PCR and Western blot, respectively. The effects of Bmi-1 knockdown on CNE-1 cells migration and invasion were analysied by Transwell migration assay and Matrigel in-vasion assay. Results Transfected with Bmi-1 siRNA significantly down-regulated the expression of Bmi-1 mRNA and protein as compared with the control group. CNE-1 cells transfected with Bmi-1 siRNA had lower levels of invasion and migration capacity than cells in the control group. Conclusion SiRNA-media-ted silencing of the Bmi-1 gene could significantly in-hibit cell migration and invasion in human nasopharyn-geal carcinoma cell line CNE-1 .
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Objective To identify the quality of life (QOL)and major influencing factors for migrant workers in Zhejiang province.Methods The QOL of 1217 migrant workers and 1387 permanent urban residents in Zhejiang province were measured by WHOQOL-BREF scale (Chinese version)in a stratified sampling.A multiple linear regression model was used to analyze the relationship between the factors and the scores of the four domains.Results There were no significant statistical differences(P＞0.05)found in the scores of physical health,social relationship and general health between migrant workers and urban residents.Their scores of psychological health,surrounding conditions and general quality of life,however,were found lower than urban residents (3.5 ± 0.8 vs 3.6 ± 0.8).Influencing factors for their psychology were education,family conflicts and appetite; those for their surrounding conditions were daily average work hours and appetite.Conclusion Given improvements in the quality of life of migrant workers,rooms to improve were found in such scores as psychological health and surrounding conditions.
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Mounting evidence supports that a newly identified regulatory T cell (Treg), CD4(+)LAP(+) Treg, is associated with oral tolerance induction and following inhibition of atherosclerosis, but little is described about whether nasal tolerance to antigen likewise induces the novel Tregs production and the relevant antiatherosclerotic benefit. We investigated the effect of nasal administration of heat shock protein-60 (HSP60) on atherogenesis. HSP60 or phosphate buffer solution (PBS) was nasally administered to six-week-old male ApoE(-/-) mice. At the 10th week after the nasal administration, there was a significant decrease in atherosclerotic plaque areas of aortic roots in the HSP60-treated mice as compared with those in the PBS-treated mice. Atherosclerosis suppression was accompanied with a significant increase in CD4(+)LAP(+) and CD4(+)CD25(+)Foxp3(+) Tregs and a concurrently increased production of TGF-β in the HSP60-treated mice. The protective effect of HSP60 was offset by injection of anti-TGF-β antibody. It is concluded that nasal administration of HSP60 can inhibit atherosclerotic formation through immune tolerance which is established by Tregs depending on the induction of anti-inflammatory cytokine TGF-β. Immune tolerance induced by nasal administration of HSP60 may provide an alternative therapeutic method for atherosclerosis.
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Objective To probe into the inequality found in primary public health service available to the migrant population and permanent residents. Methods With on-site interviews, 581 migrant workers and 581 permanent residents in a county in Zhejiang province were surveyed, to learn the inequalities between the two in the expenditure, access and outcomes of the public health service accessible to each. Results only 7. 8% of the migrant population ever had access to medical aid, a ratio far below that of the permanent residents; 40. 9% of the migrant population may turn down medical service beyond their affordability, a ratio far higher; for the migrant population, their ratio of gynecological checkup is 58. 7%, lower than that of the permanent residents (67. 7%). Conclusion Resources and financial allocation to institutions of public health should be enhanced, and social medical and insurance system should be developed and improved, in an effort to encourage the equality of primary public health services accessible to various sectors of the population.
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The concept of cause in TCM is different from the etiology concept in modern TCM. In modern TCM,the etiology is equal to pathogenic factors.At present,the significance of 'pathogenic factors'in TCM was affected by modern TCM,and this description can't show the essence of TCM theory and is not conducive to the development of cause theory of TCM.
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There are many concepts relevant to "fire" which have gradually formed during the academic development of the theories of traditional Chinese medicine.In Inner Canon of Huangdi(Huangdi Nei Jing),the concept of "fire" appears more than 240 times,including fire of yin and yang,fire from the five elements,fire from the six climatic factors,monarch fire and ministerial fire,minor fire and sthenic fire.All these lay the foundations of the theory of "fire" in traditional Chinese medicine.But the concepts of "fire" of human body which seldom appear in Inner Canon of Huangdi(Huangdi Nei Jing) have been widely discussed nowadays,such as heart fire,liver fire and stomach fire,etc.These have been created in the later ages.Monarch fire and ministerial fire now become two kinds of physiological fire of human body,which belong to the terminology of the doctrine on five element's motion and six kinds of natural factors in Inner Canon of Huangdi(Huangdi Nei Jing).
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This article unscrambled the concept of pathogenesis theory,through textual research of literatures,directly explained the concept connotation.From the relationship between pathogenesis and symptom differentiation and the innovation of pathogenesis theory of doctors in past dynasties,it demonstrated the possibility that pathogenesis theory was the innovation breakthrough point of traditional Chinese medicine theory.It proposed the conjecture that pathogenesis was the "saddle point" "sensitive point" of traditional Chinese medicine is theory,and proposed several assume about pathogenesis theory study.