Evaluation of prooxidant-antioxidant balance in in vitro fertilization-conceived mice / 대한생식의학회지

OBJECTIVE: Concerns about the safety of assisted reproductive technology (ART) have been raised, as some studies have shown elevated incidence rates of childhood cancer, asthma, allergies, and other diseases in ART-conceived babies. Findings regarding the health of ART-conceived babies are controversial. The present study was conducted to evaluate the prooxidant-antioxidant balance (PAB) in in vitro fertilization (IVF)-conceived mice in comparison to naturally conceived offspring. METHODS: Mice (6–8 weeks) were divided into two groups (IVF-conceived and naturally conceived) matched by sex, age, weight, and litter size. A 1-mL blood sample was taken and the sera were separated. The oxidant-antioxidant balance was evaluated using a fast and reliable PAB assay. The results were expressed as mean±standard deviation. RESULTS: The mean PAB values (HK units) in the IVF-conceived and naturally conceived groups were 59.70±22.30 and 54.70±18.22, respectively (p=0.82). CONCLUSION: Since free radicals contribute to several pathological conditions and antioxidants play an important protective role against oxidative stress, evaluating the oxidant-antioxidant balance is very important. Although the results of this study showed that the quality of the defense mechanism against free radicals was not significantly different between the IVF-conceived and naturally conceived mice, other parameters of metabolic dysfunction need to be measured.

Simultaneous infrared spectrometric determination of lisinopril and hydrochlorothiazide in tablets by chemometric methods

A new Fourier transform infrared [FTIR] spectrometric method was developed for assaying hydrochlorothiazide[Hctz] and lisinopril [Lnp] in binary solid pharmaceutical formulations. Initially, the mixture ofhydrochlorothiazide and lisinopril in solid mixtures was analyzed using FT-IR spectrometry in mid-IR range.Simultaneous determination of Hctz and Lnp carried out with inverse least squares [ILS] multivariate calibrationof infrared spectra of binary standards of the drugs. The spectral range of1508-1850 cm[-1] was selected for themeasurement in methanolic solutions. In this method the statistical parameters such as R[2]for Hctz and Lnp were0.9995, 0.9994 and relative standard deviation [RSD] for Hctz and Lnp were 0.71%, 0.63% [n=6], respectively.Detection limits of Lnp and Hctz were obtained 0.75 and 0.90 mg/mL, respectively. USP methods were alsoused for comparing with proposed method, as a reference method. The proposed method was successfullyapplied to the determination of these drugs in laboratory-prepared mixtures and in commercial tablets. Thismethod has suitable accuracy, precision, repeatability; and is comparable with reference standard methods