Comparative protective effect of Moringa and dandelion extracts against hepatic disorders and oxidative stress associated with prolonged use of Brufendrug in rats

Bachground: The nonsteroidal anti-inflammatory drugs [NSAIDs] are the most frequently used medications worldwide for the treatment of a variety of common chronic and acute inflammatory conditions. The association between NSAIDs and liver disease is poorly documented

Aim: the current study was carried out to investigate the hepatic disorders associated with prolonged use of Brufen drug and evaluate the role of both moringa and dandelion extracts as hepatoprotective agents against these disorders

Methods: rats were divided into six groups [six rats/ each] as follow: group 1: rats did not receive any treatment and served as control; group 2: rats orally administrated moringa extract [300mg/kg b.wt] daily for 12 weeks; group 3: rats orally administrated dandelion extract [300mg/kg b.wt] daily for 12 weeks ; group 4: rats orally administrated Brufen [18mg/kg b.wt] daily for 12 weeks;.group5: rats orally administrated moringa extract [300mg/kg b.wt] daily for one week alone then concomitant with Brufen [18mg/kg b.wt] for 11 weeks ;group 6: rats orally administrated dandelion extract [300mg/kg b.wt] daily for one week alone then concomitant with Brufen [18mg/kg b.wt] for 11 weeks

Results: the present results showed that the administration of Brufen led to significant increases in the levels of TL, TC, TG, LDL-C, ALT, AST, ALP, GGT, MDA and XO. While significant decreases in HDL-C, TP, ALP, GSH, TAC, SOD, CAT and GSH-Px, were recorded in Brufen treated rats group. On the other hand, the administration moringa or dandelion extracts succeeded to alleviate these abnormalities resulted from Brufen drug as indicated by the clear amelioration of occurred hepatic metabolic disorders, oxidative stress and histopathological changes in addition to improvement of the antioxidant status

Conclusion: it could be concluded that moringa or dandelion extracts have a remarkable role in management the hepatic disorders and oxidative stress associated with prolonged use of Brufen. Additionally it was recorded that moringa extract was more beneficial than dandelion extract in alleviating the occurred adverse effects of Brufen

role of black seed and/or bees honey in modulating the heart disorder induced by food additives in male rats

The present study aimed to throw the light on the potential hazards of some food additives whether preservatives such as sodium nitrite or colors as sunset yellow on the heart disorders through their own effect or the interaction between them. The study also, investigated the possible protective role of black seed and/or bees honey against such risks. Herein, the mentioned food additives caused significant elevations in serum and cardiac total lipids [TL], total cholesterol [TC], triglycerides [TG] and phospholipids [PL] levels as well as serum LDL-c, VLDL-c levels and LDL-c/HDL-c ratio. Meanwhile, significant decrements were shown in serum HDL-c level and HDL-c/TC ratio. Additionally, creatine kinase [CK] and aspartate transferase [AST] enzymes activity were increased significantly in serum and cardiac tissue. Moreover, oxidative stress markers; lipid peroxidation product [MDA] and protein carbonyl group [PC] were increased significantly in the cardiac tissue. Regarding, antioxidants glutathione [GSH] content, glutathione -S -transferase [GST] and catalase [CAT] enzymes activity were decreased significantly in the cardiac tissue. However, the use of black seed and/or bees honey ameliorated the disturbances observed indicating remarkable protection against the toxic effects of these food additives on the heart and offers more safety. Overall, here with the most pronounced effect was achieved by the combined treatment. In addition, the treatment by bees honey was more effective than black seed

DNA pattern and estrogen receptor content in perimenopausal dysfunctional uterine bleeding

This study was performed on 50 endometrial biopsies divided into two groups: Non-hyperplastic group [25 cases] including normal proliferative, secretory, irregular shedding, disordered proliferative and atrophic endometria and hyperplastic group [25 cases] including simple, complex and atypical hyperplastic endometria. The study aimed to correlate between estrogen receptors, DNA activity and histologic pattern in order to give an idea on the most likely type to carry the higher risk of neoplastic transformation. There was no significant association between estrogen receptor [ER] immunoreactivity and proliferative activity except for cases with atypical hyperplasia showing lower ER immunostaining and increasing S-phase fraction. Also, all types of hyperplasia had significantly higher S-phase fraction and proliferative, secretory, irregular shedding, disordered proliferative and atrophic endometria