Molecular characterization of Egyptian trichomonas vaginalis clinical isolates by HSP70 restriction fragment length polymorphism

Molecular typing of 20 Egyptian Trichomonas vaginalis clinical isolates was performed using the Restriction Fragment Length Polymorphism [RFLP] analysis employing a probe from the heat-inducible cytoplasmic HSP70 gene family hybridized with EcoR I-digested genomic DNA. In each of the isolates tested 5 to 6 distinct DNA fragments ranging from 2.7 Kb to 7.5 Kb in size were detected. Analysis of 13 isolates from symptomatic and 7 isolates from asymptomatic women revealed 6 distinct RFLP pattern subtypes of T. vaginalis. Eleven isolates [55%] showed the same RFLP pattern, teen of them [90.9%] were from symptomatic patients. T. vaginalis virus [TVV] was present in 7 isolates [35%]. Only one isolate was considered resistant to Metronidazole. There were no relations between TVV infection or Metronidazole susceptibility and RFLP subtypes

Biological variability of trichomonas vaginalis clinical isolates fom symptomatic and asymptomatic patients

The vaginal specimens of 60 women were cultured using modified thioglycolate medium. Thirteen samples were positive for Trichomonas vaginalis. The pathogenicity of these clinical isolates was investigated by intraperitoneal injection to mice. Additionally, their susceptibility to metronidazole was evaluated. All the clinical isolates produced infection in mice, but marked variations in pathogenicity and susceptibility were observed. Four out of 13 isolates produced mortality in mice, indicating a higher pathogenicity of the four isolates. Concerning metronidazole susceptibility, only one isolate was considered resistant and the other twelve showed different degrees of susceptibility

The probable relationship between toxoplasma gondii infection and cryptogenic epilepsy

Cryptogenic epilepsy is a group of epilepsy syndromes for which the etiology is unknown but the underlying brain disease is suspected. So the present work aims to study the probable relationship between Toxoplasma gondii and cryptogenic epilepsy. Fifty patient, were selected with cryptogenic epilepsy and 50 patients with known cause of epilepsy in comparision to 50 healthy as control group to investigate the sero-positivity rate for anti-To-Toxoplasma IgG antibody by ELISA. The sero-positiviry rate of anti-Toxoplasma IgG antibodies among cryptogenic epilepsy patients was [20%] and it was higher than healthy controls [2%] and known cause epilepsy patients [4%] with highly significant statistically difference [P< 0.01]. There might be a causal relationship between chronic toxoplasmosis and cryptogenic epilepsy