The primary study on the gene expression profiles of alveolar bone with traumatic occlusion in early stage in rats / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study the gene expression profiles of traumatic occlusion in early stage with the animal model of rats.</p><p><b>METHODS</b>The occlusal surface of the upper left first molar of rat was raised by placing a stainless steel wire to induce occlusal trauma in the lower left first molar. After 24 hours, the alveolar bone tissue of the first molars at the both sides of rats' lower jaws were taken out under anesthesia. The different expressive genes were shown by genome-wide microarray, which comprises about 27 000 genes and analyzed the different expressive genes with Pathway and GO analysis, finally the results of the microarray were examined by real time-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>In the results of the study, 586 different expressions were found, of which the expressions of 166 genes increased and 420 genes decreased. 106 different pathways were involved with Pathway analysis and 270 different functional classification related to GO analysis.</p><p><b>CONCLUSION</b>The balance of the lower alveolar bone is destroyed after 24 hours of traumatic occlusion. At early phase of the occlusal trauma, osteogenesis and bone formation in alveolar bone are inhibited, yet osteoblast genesis and bone resorption are not significant.</p>

The intercellular communication condition of alveolar bone with traumatic occlusion at early stage in rats / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study the intercellular communication of alveolar bone during traumatic occlusion at early stage in rats.</p><p><b>METHODS</b>The occlusal surface of the upper left first molar of rat was raised by placing a stainless steel wire to induce occlusal trauma in the lower left first molar. After 24 hours, the alveolar bone tissues of the lower jaws first molars at the both sides were taken out under anesthesia The various 27 000 genes were identified with genome-wide microarray, and further were investigated with reverse transcription-polymerase chain reaction (RT-PCR) and Pathway analysis.</p><p><b>RESULTS</b>Total 586 gene were found to be changed, 106 different signal pathways got involved with Pathway analysis, including cell adhesion molecules(CAMS), adhesions junction, gap junction, focal adhesion and tight junction, and the cytokines associated with bone metabolism in above 5 signal pathways were all down-regulated.</p><p><b>CONCLUSION</b>At the early phase of the occlusal trauma, intercellular communication in rat's alveolar bone were inhibited.</p>