ABSTRACT
We studied how consumption of vitamin C (VC) affects the oxidative stress regulation system. SMP30/GNL knockout mice (males, n = 33), which cannot synthesize VC, were randomly divided into three groups: the VC 100 Group consuming 1.5 g/L VC, the VC 2.5 Group consuming 0.0375 g/L VC, and the VC 0 Group consuming 0 g/L VC. To examine the oxidative stress regulation system, the reactive oxygen metabolites (d-ROM test) and biological anti-oxidant potential (BAP test) values were measured, and the BAP/d-ROM ratio was calculated. We obtained measurements at the beginning of the study (5 weeks old: baseline) and after VC consumption for 9 weeks (14 weeks old: 9 wk). For the plasma VC concentration, plasma reduced (ascorbic acid (AA)) and plasma oxidized (dehydroascorbic acid (DHA)) were measured at 9 wk, and the total VC (AA + DHA: total) concentration was calculated. Compared to the other groups at 9 wk, the VC 100 Group showed a significantly lower value for the d-ROM test, a significantly higher value for the BAP/d-ROM ratio, no significant difference in the BAP test, and a significantly lower senescence grading score. The VC 100 Group showed a significantly higher total VC concentration compared with the other groups. Differences in consumption of VC caused a change in the d-ROM test, the BAP/d-ROM ratio, and the plasma VC concentration.<br>
ABSTRACT
<b>Objective</b>: We studied the effect of long-term the reduced form of coenzyme Q10 (H<sub>2</sub>CoQ10: QH) consumption and exercise training (EX) with SAMP1 mice on senescence, exercise capacity, and an oxidative stress regulation system.<br> <b>Methods</b>: The subjects of this study were 50 SAMP1 mice for accelerated senescence, sorted at random into four groups: one which consumed QH, one which consumed QH and performed EX, one which performed EX, and a control group. The experiment started when the mice were 8 weeks old and the consumption of QH continued for 12 months (12M). The measurement for the oxidative stress regulation system was performed at the start of this study and 12M later. Running time for exercise capacity, senescence grading scores, and body weight were each measured at the start of the study, 6 months (6M) and 12M later. For the oxidative stress regulation system, we used reactive oxygen and free radical analysis equipment, with which we measured plasma oxidative stresses (d-ROM test) and plasma anti-oxidant potential (BAP test), and calculated the ratio of d-ROM to BAP.<br> <b>Result</b>: A combination of QH consumption and EX showed, at a later stage, an inhibiting effect on the increase in senescence grading scores and on the decrease in running time. For the oxidative stress regulation system, the combination showed a decrease in latent anti-oxidant potential caused by the rise of plasma oxidative stresses.<br> <b>Conclusion</b>: Results of this study suggest that a combination of QH consumption and EX is effective in delaying aging, inhibiting the decline of exercise capacity, and decrease in latent anti-oxidant potential.<br>
ABSTRACT
<b>Objective</b>: We studied the effect of food consumption and exercise training (EX) on the oxidative stress regulation system and exercise capacity in mice.<br> <b>Methods</b>: As subjects of this study, we used 46 male mice, which were classified randomly into four groups: one that consumed the reduced coenzyme Q10 (H<sub>2</sub>CoQ10: QH), one that consumed QH and took EX, one that took EX, and a control group. All the groups were set to keep running to their limit on a treadmill for animals. We measured the exercise capacity (running time) of each group at the beginning and after three, six, and nine months of this study. For the oxidative stress regulation system, we used reactive oxygen and free radical analysis equipment at the beginning and 6 months later of this study. We measured plasma oxidative stresses (d-ROM test) and plasma anti-oxidant potential (BAP test) to calculate the ratio of d-ROM to BAP. After nine months of this study we measured plasma anti-oxidants (ANTI-ROM test), values of plasma QH and plasma Q10 to calculate the reduced ratio.<br> <b>Result</b>: As a short-term effect of exercise capacity, prolonged running time were recognized by a combination of QH consumption and EX, and as a long-term effect, prolonged running time was recognized by QH consumption. For the oxidative stress regulation system, d-ROM test and ANTI-ROM test did not show any effect of QH consumption or combination of QH consumption and EX. However an elevated value was indicated in the reduced ratio by a combination of QH consumption and EX.<br> <b>Conclusion</b>: This study has revealed that a combination of QH consumption and EX has an effect on exercise capacity and the oxidative stress regulation system such as reduced ratio.<br>