ABSTRACT
The aim of this study was to evaluate the diagnostic value of Dsg1 and 3 enzyme-linked immunosorbent assay [EL1SA] in the diagnosis of pemphigus. Thirty-six patients with pemphigus were enrolled. The diagnosis was confirmed by histopathology and direct immunofluorescence [DIF]. Autoantibodies against Dsg1, and Dsg3 were assayed, by ELISA, in serum of pemphigus patients. In pemphigus vulgaris [PV], anti-Dsg1 and anti-Dsg3 antibodies titer were raised above the cut off values 88.5% and 96.1% of patients respectively. Dsg-3 was more sensitive than Dsg-1[96.2% 88.5%] while both be the same specificity 95%. In PF and PH, the only anti-Dsg1 level was in all cases [100%]. Conclusion: In PV Dsg-3 was more sensitive than Dsg1 while in and pemphigus herpetiformis, only anti-Dsg-1 was detected in all cases [100%]. Both anti-Dsg1Ab and anti-Dsg3Ab ELISA values are correlated with the disease activity
ABSTRACT
Background: immunofluorescence [IF] is a reliable biochemical staining technique for the detection of antibodies, which are bound to antigen in the tissue or circulating in body fluids. The relative simplicity and accuracy of the technique has made immunofluorescence an unavoidably powerful technique in the diagnosis of bullous diseases
Objectives: to detect the pattern of direct imrnunofluorescence [DIF] in the diagnosis of pemphigus
Methods: thirty-six patients with pemphigus disease were enrolled in our study. The age of whom rangedfrom 21to 73 years with a mean age +/- SD of 46.94 +/- 14.6years Two skin biopsies [one from the bullous lesion and the other one from adjacent area] were taken from each patient, lesional biopsy specimens were used for histopathological examination after standard processing, while perilesional skin samples were used for performing direct immunofluorescence
Results: DIF was positive in all patients with pemphigus vulgaris [PV], showing intraepidermal deposition of immunoglobulins and complement 3 [C[3]]. IgG was seen in all of them [100%], while C[3] was also observed in 38.4% patients, and IgM was positive in [3.8%] in a similar pattern
Conclusion: immunofluorescence [IF] studies are an important part of the laboratory evaluation of immunologically mediated bullous dermatoses and have become standard procedures for accurately diagnosing patients with these disorders
ABSTRACT
To investigate The effects of cryopreservation on sperm motility, vitality and DNA integrity in fresh and processed sperms. Pre-cryopreservation and post-cryopreservation analysis of sperm vitality, motility and DNA integrity in fresh and processed semen. Department of Dermatology, Venereology and Andrology, in coordination with the Department of Clinical Pathology Assiut University Hospital. Patients: 50 fertile men [within the last year] who are clinically free and with normal semen parameters the, semen samples collected by masturbation into sterile containers after at least 3 days of sexual abstinence. Semen evaluation for, conventional semen analysis, sperm vitality with Hypo-Osmotic Swelling test [HOS], sperm DNA integrity by flowcytometry. Each sample was divided into 2 halves: The first half was cryoperserved without processing. The second half of the sample was processed by swim up technique. HOS test, percentage of progressive motility and DNA integrity after processing. Cryopreservation in liquid nitrogen for at least 24 hours for all samples [fresh and processed,] was done. HOS test, percentage of progressive motility and DNA integrity after thawing for all samples [fresh and processed]. Sperm DNA fragmentation index was determined using flowcytometry, sperm vitality was determined using HOS test and percentage of progressive sperm motility was determined using light microscopic examination according to criteria of WHO [1999]. Sperm frozen after processing had higher resistance to freezing damage as regards vitality and motility when compared with sperm frozen without processing however, sperm DNA fragmentation index was more in frozen processed than unprocessed sperms. Cryopreservation results in decreased sperm vitality, motility and increased sperm DNA fragmentation. Freezing processed sperm give better post-thawing vitality and motility but, increased sperm DNA fragmentation when compared with unprocessed sperm