Preparation and cytotoxic evaluation of magnetite [Fe[3]O[4]] nanoparticles on breast cancer cells and its combinatory effects with doxorubicin used in Hyperthermia

Magnetic nanoparticles in a variable magnetic field are able to produce heat. This heat [42-45[degree sign]C] has more selective effect on fast dividing cancer cells than normal tissues. In this work magnetite nanoparticles have been prepared via coprecipitation and phase identification was performed by powder x-ray diffraction [XRD]. Magnetic parameters of the prepared nanoparticles were measured by a Vibrating Sample Magnetometer [VSM]. A sensitive thermometer has been used to measure the increase of temperature in the presence of an alternating magnetic field. To evaluate the cytotoxicity of nanoparticles, the suspended magnetite nanoparticles in liquid paraffin, doxorubicin and a mixture of both were added to the MDA-MB-468 cells in separate 15 ml tubes and left either in the RT or in the magnetic field for 30 min. Cell survival was measured by trypan blue exclusion assay and flow cytometer. Particle size distribution of the nanoparticles was homogeneous with a mean particles size of 10 nm. A 15[degree sign]C temperature increase was achieved in presence of an AC magnetic field after 15 min irradiation. Biological results showed that magnetite nanoparticles alone were not cytotoxic at RT, while in the alternative magnetic filed more than 50% of cells were dead. Doxorubicin alone was not cytotoxic during 30 min, but in combination with magnetite more than 80% of the cells were killed. It could be concluded that doxorubicin and magnetite nanoparticles in an AC magnetic field had combinatory effects against cells

In-vitro comparison of cytotoxicity of two bioactive glasses in micropowder and nanopowder forms

The cytotoxicity of the biomaterials is a key issue that should be addressed prior to pre-clinical applications. This study was designed to evaluate and compare the cytotoxixity of two forms of bioactive glasses:nanopowder: and micropowder. Human HGF1-PI53 gingival fibroblast cells were used to evaluate the cytotoxicity of 0.5, 1, 1.5, 2, 5, 10, 15 and 20 mg/mL concentrations of the two bioactive glasses via MTT assay. The results were statistically analyzed using analysis of variance and Tukey's test. A p-value less than 0.05 was considered statistically significant. Results showed that two bioactive glasses hadstatistically significant differences at 5, 10, 15 and 20 mg/mL concentrations [p-value < 0.05] and there was no correlation between time and cell cytotoxicity of bioactive glasses [p-value > 0.05], using t-test and Spearman's correlation coefficient. We conclude that that cytotoxicity of nanopowder bioactive glass at concentrations /= 5 mg/mL in the first 48 h of applications

In vitro cytotoxicity of two subspecies of juniperus excelsa on cancer cells

The cytotoxic effects of crude ethanol extracts of some previously tested Iranian conifers on tumor cell lines have motivated us to screen different parts of two subspecies in these genus. Terminal branchlets and berries of Juniperus excelsa subsp. excelsa and J. excelsa subsp. polycarpos were collected, dried and extracted with ethanol/H2O [80/20 v/v] via percolation procedure. Extracts were dried, reconstituted in ethanol and cytotoxic effects of different concentrations were determined on cancer cells by ELISA, using MTT assay. MDA-MB-468, Hela and KB cells were used in this study. The extracts of the branchlets of male and female of J. excelsa subsp. polycarpos as well as berries extract of J. excelsa subsp. excelsa showed inhibitory activities against KB cells. Extracts of female branchlets and berries of J. excelsa subsp. polycarpos were cytotoxic against all 3 cell lines. In conclusion, obtained extracts from J. excelsa subsp. polycarpos showed cytotoxic effects against most tested cell lines which was comparable to doxorubicin; whereas, berries extracts of J. excelsa subsp. Excels showed inhibitory effects only against KB cells