ABSTRACT
<p><b>OBJECTIVE</b>To observe the impact of Roux-en-Y gastric bypass on quality of life in non-obese patients with type 2 diabetes mellitus.</p><p><b>METHODS</b>Thirty-seven non-obese patients with type 2 diabetes mellitus who underwent Roux-en-Y gastric bypass were prospectively studied. A 36-item short form healthy survey questionnaire(SF36), the diabetes treatment satisfaction questionnaire(DTSQ), and quality of life scale for patients with type 2 diabetes mellitus(DMQLS) were used to evaluate the quality of life for all the non-obese patients with type 2 diabetes mellitus preoperatively and 12 months postoperatively.</p><p><b>RESULT</b>The blood glucose and lipid indexes were significantly decreased after operation(all P<0.05). SF36 showed the physical and mental synthesis scores at 12 month after operation were 74.6±18.3 and 79.8±14.9 respectively, higher than those at one week before operation(54.9±15.1 and 56.4±17.8, both P<0.01). DTSQ showed treatment satisfaction score was increaced significantly after operation(29.2±7.1 vs. 15.4±5.6, P<0.01). The quality of life evaluated by DMQLS, was also significantly improved(P<0.01).</p><p><b>CONCLUSION</b>Roux-en-Y gastric bypass can significantly improve the quality of life for non-obese patients with type 2 diabetes mellitus.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Diabetes Mellitus, Type 2 , Psychology , General Surgery , Gastric Bypass , Methods , Psychology , Laparoscopy , Methods , Psychology , Obesity , Quality of Life , Retrospective Studies , Surveys and Questionnaires , Treatment OutcomeABSTRACT
Objective: To observe the tolerance to ischemia-reperfusion injuries in different parts of extrahepatic bile duct during orthotopic autologous liver transplantation in rats, so as to lay a foundation for selection of anastomosis site during liver transplantation. Methods: Thirty SD rats were randomly divided into 3 groups: group I, sham operated(n=6); group II, 1 h ischemia in biliary tract followed by 1 h reperfusion (n=12); and group III, 1 h ischemia in biliary tract followed by 2 h reperfusion(n=12). TUNEL assay, pathomorphology score and ultrastructural quantitative analysis were used to analyze the epithelia of hilar bile duct, proximal, and distal common bile duct. Results: The results of TUNEL assay and pathomorphology score were not significantly different between the proximal and distal common bile ducts in group II (P>0.05), but the injury was more serious in hilar bile duct compared with those of the proximal and distal common bile ducts (P<005); the hilar bile duct had the most serious injuries as indicated by the changes of mitochondrial mean volume (V) and area density of microvilli (AMV), and the proximal common bile duct had the slightest injury (P<0.05). In group 1, the most severe injury was found in the hilar bile duct, followed by distal common bile duct and proximal common bile duct in order (P<0.05). Conclusion: The tolerance to ischemia-reperfusion is different in different parts of bile duct during liver transplantation, with the distal common duct having the best tolerance, which may be used for bile anastomosis during transplantation.
ABSTRACT
Objective: To establish an orthotopic autologous liver transplantation model with bile duct ischemia-reperfusion injury in rats, so as to make it easy for the operation. Methods: Eighty SD rats were used to establish the models. The method used for this model was the same as the one for orthotopic liver transplantation, except for the blood vessel anastomosis and the constant pressure perfusion of portal vein and abdominal aorta. The portal vein and the hepatic artery reperfusion were controlled by artery clamp. Results: The successful rate of the operation for establishing the model was 95%(76/80) and the anhepatic phase was (16±2) min. The periods of hot and cold ischemia and reperfusion could be accurately controlled and the effect of double constant pressure perfusion was good. There was no erythrocyte in the capillary of bile ductal wall under the light microscope after the reperfusion. Conclusion: This technique, simulating the whole process of liver transplantation, is simple and has high successful rate. Besides, it rules out the influence of immune agent on bile ducts impairment, and better reflects the pathophysiological process of bile ducts ischemia and reperfusion injury,providing a novel approach for investigation of bile ducts injury during liver transplantation.
ABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of Ginseng Sini Tang.</p><p><b>METHOD</b>The constituents were identified by physico-chemical properties and spectral analysis.</p><p><b>RESULT</b>The 12 compounds were identified as ginsenoside-Rb1,-Rb2,-Rb3,-Rc,-Rd,-Re,-Rg1,Rg2,Rg3,Rf,Ra1,Ra2. The 10 compounds were identified as benzoylmesaconitine(BM), benzoylaconitine(BA), benzoylhypaconitine(BH), neoline (NL), fuziline (FL), 14-ethyl-talatisamine14-acetyl-talatisamine (AT), 14-benzoylhypaconine-8-linoleate (HAL),14-benzoyldeoxyaconine-8-oleate(HAO), 14-benzoylhypaconine-8-palmitate(HAP), talatisamine(TS).</p><p><b>CONCLUSION</b>All these compounds were obtained from Ginseng Sini Tang for first times.</p>