ABSTRACT
OBJECTIVE@#To filtrate and prove the different microRNAs (miRs) profiles in nasopharyngeal carcinoma.@*METHOD@#Screening the different expressions of miRs between nasopharyngeal carcinoma and the inflammatory tissues by the application of expression profiling of chip high-throughput and large-scale microarray analysis. Then we used RT-QPCR technology to prove the accuracy of screening results.@*RESULT@#There were significant expression differences of miRs between nasopharyngeal carcinoma and the control tissues, 144 human miRs had 2 or more fold the difference ratio. Compared with the inflammatory tissues, we have found that miRs-34b, miRs-449b and miRs-7-1 significantly low expressed in nasopharyngeal carcinoma, yet miRs-125b, miRs-184, miRs-196b, miRs-205 and miRs-24-1 expressed high. The results were consistent with the microarray analysis.@*CONCLUSION@#The difference expressed miRs might be closely related to the process of nasopharyngeal carcinoma, and the research on miRs profiles maybe provide a powerful target basis for early diagnosis and therapy of nasopharyngeal carcinoma.
Subject(s)
Humans , Carcinoma , Gene Expression Profiling , MicroRNAs , Genetics , Nasopharyngeal Carcinoma , Nasopharyngeal Neoplasms , Genetics , Oligonucleotide Array Sequence AnalysisABSTRACT
Objective To investigate the clinical significance of combned detection of serum pre-S1 ,pre-S2 antigen and hepatitis B virus(HBV) e antigen(HBeAg) for diagnosis of HBV infection .Methods Enzyme-linked immunosorbent assay(ELISA) was employed to detect the serum pre-S1 ,pre-S2 antigen and HBeAg of 450 patients with chronic hepatitis B and real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to detect their HBV DNA .Results In patients with different HBV DNA levels ,serum pre-S1 ,pre-S2 antigen and HBeAg showed different expression ,the higher the load ,the higher the expression (P<0 .05) .Pre-S1 ,pre-S2 antigen and HBeAg were all correlated with HBV DNA (P<0 .05) and the descending order of rele-vance were HBeAg ,pre-S1 and pre-S2 antigen .Combied detection of the three indicators showed the highest correlation with HBV DNA .The positive proportion of HBV DNA of patients with the three indicators positive was markedly higher than those in pa-tients with single or two indicators positive (P<0 .05) .Conclusion Combind detection of serum pre-S1 ,pre-S2 antigen and HBeAg may reflect well on HBV replication .