ABSTRACT
The erythrocyte sodium content and sodium transport in 50 cases of diabetic patients (NIDDM) and 26 cases of normal subjects were determined. The results showed that the sodium content of erythrocyte was obviously higher in the diabetic patients than that in the normal subjects, the activity of Na-pumps of erythrocyte was obviously lower in diabetic patients than that in normal subjects, but there was no significant difference of permeability of erythrocyte membrane to sodium observed. The changes of erythrocyte sodium content and the activity of Na-pumps of erythrocyte were obviously greater in female diabetic patients than those in male diabetic patients. The addition of insulin in vitro at a concentration of 5mg/L significantly decreased the erythrocyte sodium content and increased the activity of Na-pumps of erythrocyte in diabetic patients.
ABSTRACT
The fluidity of membrane lipid regions of intact human erythrocyte in diabetic patients and healthy subjects was determined by means of fluorescense polarization technique using 1,6-dipheny1-1, 3, 5- hexatriene (DPH) as fluorescence probe. The results showed that the fluidity of membrane lipid regions of intact erythrocyte in diabetic patients was lower than that in healthy subjects. The fluorescence polarization and microviscosity of diabetic patients (n= 15) were 0.259 + 0.001 and 2.568 +0.019 respectively; while that of healthy subjects (n = 15) were 0.246 + 0.002 and 2.304 + 0.042 respectively (P