ABSTRACT
Acute myeloid leukemia [AML] is defined as leukemic blast reproduction in bone marrow. Chromosomal abnormalities form different subgroups with joint clinical specifications and results. t[8;21][q22;q22] and inv[16][p13;q22] form core binding factor- AML [CBF-AML]. c-kit mutation activation occurs in 12.8-46.1% of adults with CBF leukemia. These mutations occur in 20-25% of t[8;21] and 30% of inv[16] cases
Methods: In this systematic review, we searched different databases, including PubMed, Scopus, and Embase. Selected articles were measured based on the inclusion criteria of this study and initially compared in terms of titles or abstracts. Finally, articles relevant to the subject of this review were retrieved in full text. Twenty-two articles matched the inclusion criteria and were selected for this review
Results: In this study, c-kit mutations were associated with poor prognosis in AML patients with t[8;21] and inv[16]. In addition, these mutations had better prognostic effects on AML patients with inv[16] compared with those with t[8;21]
Conclusion: According to the results of this study, c-kit mutations have intense, harmful effects on the relapse and white blood cell increase in CBF-AML adults. However, these mutations have no significant prognostic effects on patients
ABSTRACT
Background and Aims: multiple myeloma is a malignant proliferation of plasma cells derived from a single clone. The tumor, its products and the host response lead to organ damages. Some factors that are responsible in its pathogenesis are recognized. As FMS like Tyrosine Kinase 3 receptor [FLT3] mutation has been proved as a determining factor in leukemic patients; the goal of this study was to find association of FLT3 internal tandem duplication [ITD] and FLT3 tyrosine kinase domain [TKD] mutations with multiple myeloma
Materials and Methods: this case-control study was conducted on 60 paraffinembedded bone marrow biopsies [30 multiple myeloma and 30 normal bone marrow specimens] in the pathology departments of Ghaem and Imam Reza hospitals in Mashhad. After sections preparation, DNA was extracted and two PCR reactions were set up for detection of FLT3/ ITD and FLT3/TKD mutations
Results: the Mean age of samples was 64+/-10 years. No FLT3 mutations were detected in multiple myeloma patients
Conclusion: our findings showed that occurrence of FLT3 mutations seem unusual in multiple myeloma
ABSTRACT
Introduction: Chronic obstructive pulmonary disease [COPD] secondary to sulfur mustard gas poisoning, known as mustard lung, is a major late pulmonary complications in chemical warfare patients. Serious comorbidities like dyslipidemia are frequently encountered in COPD. The aim of this study was to measure the serum lipid profile and evaluate the relation of lipid parameters with the severity of airway obstruction in mustard lung patients
Materials and Methods: Thirty-six non-smoker mustard lung patients with no history of cardiovascular disease, diabetes mellitus, and dyslipidemia were entered into this cross-sectional study. Control group consisted of 36 healthy non-smoker men were considered in this study. Serum lipid profile was performed in the patients and the controls. Spirometry was done in mustard lung patients
Results: The mean age of the patients was 47 +/- 6.80 SD years. The mean duration of COPD was 18.50 +/- 7.75 SD years. There were statistically significant differences in mean serum triglycerides and total cholesterol levels between patients and controls [P=0.04 and P=0.03, respectively].The mean levels of lipid parameters were not statistically significant different among the 4 stages of COPD severity [P>0.05]
Conclusion: The current study revealed that the serum levels of triglycerides and cholesterol are elevated in mustard lung patients compared with the healthy controls. Since lipid profile abnormalities are considered as a major risk factor for cardiovascular disease, especial attention to this matter is recommended in mustard lung patients
ABSTRACT
Introduction: Sulfur mustard [SM], a toxic alkylating gas, can cause serious long-term pulmonary complications such as chronic obstructive pulmonary disease [COPD]. Metabolic syndrome [MetS] is one of the important comorbidities of COPD. This study was designed to evaluate the frequency of metabolic syndrome in Iranian chemical warfare patients [CWPs] with COPD
Materials and Methods: Thirty CWPs with a mean age of 46.93 +/- 6.8 were enrolled in this study. The following parameters were studied in: complete pulmonary function tests, health-related quality of life, serum triglycerides [TG], high density lipoprotein [HDL] and fasting blood sugar [FBS] levels. Additionally, 32 COPD patients and 56 healthy persons were considered as control groups who were matched to CWPs
Results: We found a statistically significant difference in the frequency of MetS between the COPD patients and the healthy control group [P=0.04]. Additionally, we observed a statistically significant difference in the mean HDL levels among these groups [P=<0.001]. In the CWPs, the frequency of MetS was significantly decreased in severe to very severe stages [P<0.001]
Conclusion: Our data indicate that metabolic syndrome is frequent in chemical warfare patients, and special attention to this condition in mild to moderate stages is recommended
ABSTRACT
The physiological changes in thyroid gland during pregnancy have been suggested as one of the pathophysiologic causes of preeclampsia. The aim of this study was comparison of serum levels of Tri-iodothyronine [T3], Thyroxine [T4], and Thyroid-Stimulating Hormone [TSH] in preeclampsia and normal pregnancy. In this case-control study, 40 normal pregnant women and 40 cases of preeclamsia in third trimester of pregnancy were evaluated. They were compared for serum levels of Free T3 [FT3], Free T4 [FT4] and TSH. The data was analyzed by SPSS software with the use of t-student, Chi-square, Independent sample T-test and Bivariate correlation test. P
Subject(s)
Humans , Female , Pre-Eclampsia/blood , Pre-Eclampsia/physiopathology , Triiodothyronine/blood , Thyroxine/blood , Thyroid Function Tests , Pregnancy Trimester, Third , Case-Control Studies , Pregnancy/bloodABSTRACT
Background: Iron-deficiency anaemia (IDA) is a major worldwide public health problem. Children and women of reproductive age are especially vulnerable to IDA, and it has been reported that these patients are more prone to infection. This study was done to evaluate alteration of lymphocyte subgroups in IDA. Methods: In this prospective study, we investigated lymphocyte subsets in pre-menopausal women with iron-deficiency anaemia; 50 normal subjects and 50 IDA (hypochromic microcytic) cases were enrolled. Experimental and control anticoagulated blood samples were evaluated using flow cytometry to determine the absolute and relative numbers of various lymphocyte subgroups. Finally, the results of the patient and control groups were compared. Results: Mean (SD) absolute counts of lymphocytes, CD3+ cells, CD3+/CD4+ subsets (T helper) and CD3+/CD8+ subsets (T cytotoxic) in the patient group were 2.08 (0.65) x 109/L, 1.53 (0.53) x 109/L, 0.87 (0.28) x 109/L, and 0.51 (0.24) x 109/L, respectively. The results showed significant differences between case and control groups in mean absolute counts of lymphocytes (P = 0.014), T lymphocytes (P = 0.009), helper T cells (P = 0.004), and cytotoxic T cells (P = 0.043). Conclusion: This study showed that absolute counts of peripheral blood T lymphocytes as a marker of cell-mediated immunity may be decreased in pre-menopausal women with iron-deficiency anaemia, and that these patients may be more prone to infection.
ABSTRACT
To investigate the possible association between hyperhomocysteinemia and preeclampsia. A case-control study was carried out in the Departments of Obstetrics and Gynecology of the Ghaem Hospitals in Mashhad University of Medical Sciences, Mashhad, Iran from May 2004 to August 2006 and included 75 preeclamptic patients, 37 women with mild preeclampsia, and 38 women with severe preeclampsia, in addition we included 40 controls without pregnancy complications. Plasma total homocysteine was determined in all subjects by enzyme linked immunosorbent assay. The 3 groups were similar in age and body. Patients with severe preeclampsia had significantly higher mean plasma levels 13.8 +/- 7mg/l than normal pregnant women 8.8 +/- 2.8mg/l and mild pre-eclamptic women 10.4 +/- 2.3mg/l p < 0.05. Women who developed severe preeclampsia have higher plasma homocysteine levels than women who remain normotensive throughout pregnancy
Subject(s)
Humans , Female , Homocysteine/blood , Pregnancy , Case-Control Studies , Enzyme-Linked Immunosorbent AssayABSTRACT
The aim of this study was to compare the sensitivity and specificity of Acid fast and Auramine-Rhodamine staining and Multiplex PCR for the detection of Mycobacterium tuberculosis complex and non tuberculosis Mycobacteria on formalin fixed paraffin embedded tissues [FFPE]. Forty cases of FFPE pleural and bronchial tissue with chronic granulomatous inflammation and caseous necrosis and 10 cases with bronchogenic carcinoma as controls were investigated. We designed a Multiplex PCR DNA amplification method with two targets: 123bp DNA fragment from IS6110, which is present only in mycobacterium tuberculosis complex and 162bp DNA encoding Ag 85complex which is present in all of mycobacteria. The FFPE also stained by Acid fast and Rhodamine-Auramine staining method. In 26 samples [65%] 123 bp and 162 bp DNA fragments were detected together [12 in bronchial samples and 14 in pleural samples].The 162 bp fragment wasn't detected alone. The sensitivity of PCR was 65% and the specificity was 100%. Eleven cases were positive for Acid fast staining. There was 27.5% sensitivity and 100% specificity. Thirteen cases were positive for Auramine-Rhodamine staining [A-R-S]; there was 32.5% sensitivity and 100% specificity. All of the 10 controls were negative for 123 bp, 162 bp DNA fragments, for Acid fast and Auramine-Rhodamine staining. Multiplex PCR is a sensitive, specific and rapid method for detection of M tuberculosis in FFPE tissues