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Journal of Forensic Medicine ; (6): 196-203, 2006.
Article in Chinese | WPRIM | ID: wpr-983179

ABSTRACT

OBJECTIVE@#The STR genotypping of trace oral epithelial cells which are microdissected by laser capture microdissection system (LCM) is explored.@*METHODS@#The oral epithelial cells are microdissected using a low-power infrared laser by VERITAS Microdissection Instrument. STR loci of Profiler Plus are detected by multiplex PCR procesures.@*RESULTS@#DNA genotyping of 7-8 oral epithelial cells are succeeded, and DNA genotyping of 3-4 oral epithelial cells are failed.@*CONCLUSION@#It is viable in genotyping of trace oral epithelial cells by Laser Capture Microdissection as a new technology of seperating single cell.


Subject(s)
Humans , Cell Separation/methods , DNA/genetics , Epithelial Cells , Genotype , Lasers , Microdissection/methods , Mouth/cytology , Tandem Repeat Sequences
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