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1.
Journal of the Korean Society of Magnetic Resonance in Medicine ; : 75-75, 2014.
Article in English | WPRIM | ID: wpr-223479

ABSTRACT

We found an error in our published article.

2.
Journal of the Korean Society of Magnetic Resonance in Medicine ; : 47-54, 2012.
Article in English | WPRIM | ID: wpr-185403

ABSTRACT

PURPOSE: This study was performed to evaluate the characteristics of rat mesenchymal stem cells (RMSCs) transduced with human ferritin gene and investigate in vitro MRI detectability of ferritin-transduced RMSCs. MATERIALS AND METHODS: The RMSCs expressing both myc-tagged human ferritin heavy chain subunit (myc-FTH) and green fluorescence protein (GFP) were transduced with lentiviurs. Transduced cells were sorted by GFP expression using a fluorescence-activated cell sorter. Myc-FTH and GFP expression in transduced cells were detected by immunofluorescence staining. The cell proliferative ability and viability were assessed by MTT assay. The RMSC surface markers (CD29+/CD45-) were analyzed by flow cytometry. The intracellular iron amount was measured spectrophotometically and the presence of ferritin-iron accumulation was detected by Prussian blue staining. In vitro magnetic resonance imaging (MRI) study of cell phantoms was done on 9.4 T MR scanner to evaluate the feasibility of imaging the ferritin-transduced RMSCs. RESULTS: The myc-FTH and GFP genes were stably transduced into RMSCs. No significant differences were observed in terms of biologic properties in transduced RMSCs compared with non-transduced RMSCs. Ferritin-transduced RMSCs exhibited increased iron accumulation ability and showed significantly lower T2 relaxation time than non-transduced RMSCs. CONCLUSION: Ferritin gene as MR reporter gene could be used for non-invasive tracking and visualization of therapeutic mesenchymal stem cells by MRI.


Subject(s)
Animals , Humans , Rats , Apoferritins , Ferritins , Ferrocyanides , Flow Cytometry , Fluorescence , Fluorescent Antibody Technique , Genes, Reporter , Iron , Magnetic Resonance Imaging , Mesenchymal Stem Cells , Relaxation , Track and Field
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