ABSTRACT
Resistance among bacterial isolates is the leading cause of increased mortality and morbidity worldwide. Carbapenems once thought to be effective are becoming ineffective mostly due to the emergence of carbapenemase. This study was designed to determine in vitro efficacy of Modified Hodge test for detection of carbapenemase production in Gram negative rods. The study was done in the Department of Microbiology, Armed Forces Institute of Pathology Rawalpindi Pakistan from January 2010 to December 2010. A total of 200 Gram negative rods from different clinical samples were taken. Those isolates which showed intermediate or susceptible zones i.e 16mm-21mm on disc diffusion were included in the study. These isolates were then subjected to Modified Hodge test. Out of 200 isolates, 138 [69%] were positive for carbapenemase production by Modified Hodge test. Out of 138 MHT positive organisms, the frequency of E. coli was 38%, followed by Pseudomonas aeruginosa [30%], Klebsiella pneumoniae [17%], Acinetobacter baumannii [12%], Citrobacter diversus [2%] and Enterobacter agglomerans [1.4%]. Modified Hodge test is a simple test which can be performed in the routine lab for detection of carbapenemases in isolates showing intermediate or sensitive zone diameter on disc diffusion
Subject(s)
Bacterial Proteins , Carbapenems , Drug Resistance, Bacterial , Microbial Sensitivity TestsABSTRACT
100 gastric biopsies, in two transport media, were homogenized and cultured on three media under microaerophilic conditions. Columbia agar with commercial antibiotic supplement [containing 10 mg Vancomycin, 05mg trimethoprim, 05 mg cefsulodin and 05 mg ampotericin B per litre] yielded the best results. Growth was obtained in 3-7 days. The resistance to nalidixic acid and rapid urease production of H. pylon could be used to differentiate this species form cainpylobacter spp. Metronidazole resistance was found in 27/43[63%] of isolates. All the isolates were found to be sensitive to Amoxycillin, Clarithromycin and Tetracycline