ABSTRACT
The study describes a comparison between molecular hybridization using a non-radiolabeled, thymine-thymine (TT) dimerized synthetic oligonucleotide complementary DNA probe and electron microscopy for the detection of hepatitis E virus genome in bile. Spot hybridization with the TT dimerized probe was found to be more sensitive and specific compared to electron microscopy.
Subject(s)
Animals , Bile/microbiology , DNA, Complementary , DNA, Viral , Genome, Viral , Hepatitis E/microbiology , Hepatitis E virus/genetics , Microscopy, Electron/methods , Monkey Diseases/microbiology , Nucleic Acid Hybridization/methods , Oligonucleotide Probes , Pyrimidine Dimers , Sensitivity and Specificity , Time FactorsABSTRACT
The study described the spot hybridization technique using a non-radiolabelled, thymine-thymine dimerized synthetic oligonucleotide complementary DNA(cDNA) proble for the detection of hepatitis E virus genome in bile. The test performed well with high degree of sensitivity and specificity. It was compared with the conventional electron microscopy and was found to be thousand times more sensitive.