Delayed SARS-COV-2 clearance in infected persons in Ghana

Objective: This study aimed to determine the duration of SARS-CoV-2 clearance in persons in Ghana. The research question was whether the duration of virus clearance in Ghana matched the 14 days recommended by the World Health Organization (WHO); this had direct implications for transmission, which was key in managing the COVID-19 pandemic. Design: This was a retrospective analytical study. Setting: All facilities that submitted clinical specimens to Noguchi Memorial Institute for Medical Research (NMIMR) for SARS-CoV-2 diagnosis between March to June 2020 were included in the study. Interventions: Samples from 480 persons who tested positive for SARS-CoV-2 by RT-PCR from March to June 2020 at NMIMR and submitted at least two follow-up samples were retrospectively analysed. Individuals with two consecutive negative RT-PCR retesting results were considered to have cleared SARS-CoV-2. Results: The median time from the initial positive test to virus clearance was 20 days (IQR: 5-56 days). This was six days longer than the WHO-recommended 14 days, after which infected persons could be de-isolated. Sputum and nasopharyngeal swabs proved more sensitive for detecting viral RNA as the infection progressed. At a significance level of 0.05, age and sex did not seem to influence the time to SARS-CoV-2 clearance. Conclusions: The median time to SARS-CoV-2 clearance in this study was 20 days, suggesting that SARS-CoV-2 infected persons in Ghana take longer to clear the virus. This finding calls for further investigations into whether patients who remain PCR positive continue to be infectious and inform isolation practices in Ghana.

Dysregulation in erythrocyte dynamics caused by SARS-CoV-2 infection: possible role in shuffling the homeostatic puzzle during COVID-19

Abstract Introduction The evolving COVID-19 pandemic became a hallmark in human history, not only by changing lifestyles, but also by enriching scientific knowledge on viral infection and its consequences. Objective Although the management of cardiorespiratory changes is pivotal to a favorable prognosis during severe clinical findings, dysregulation of other systems caused by SARS-CoV-2 infection may imbalance erythrocyte dynamics, such as a bidirectional positive feedback loop pathophysiology. Method and Results Recent evidence shows that SARS-CoV-2 is capable of affecting the genetics and dynamics of erythrocytes and this coexists with a non-homeostatic function of cardiovascular, respiratory and renal systems during COVID-19. In hypothesis, SARS-CoV-2-induced systematical alterations of erythrocytes dynamics would constitute a setpoint for COVID-19-related multiple organ failure syndrome and death. Conclusion The present review covers the most frequent erythrocyte-related non-homeostatic findings during COVID-19 capable of providing mechanistic clues of SARS-CoV-2-induced infection and inspiring therapeutic-oriented scientific evidence.

Effects of a psychological intervention on the quality of life of obese adolescents under a multidisciplinary treatment / Efeitos de uma intervenção psicológica sobre a qualidade de vida de adolescentes obesos em tratamento multidisciplinar

Abstract Objective: To investigate the effects of multidisciplinary treatment with and without psychological counseling on obese adolescents' self-reported quality of life. Methods: Seventy-six obese adolescents (15.87 ± 1.53 y) were allocated into psychological counseling group (PCG; n = 36) or control group (CG; n = 40) for 12 weeks. All participants received the same supervised exercise training, nutritional and clinical counseling. Participants in PCG also received psychological counseling. QOL was measured before and after 12 weeks of intervention by Generic Questionnaire for the Evaluation of Quality of Life (SF-36). Results: The dropout rate was higher in GC (22.5%) when compared with PCG (0.0%) (p < 0.001). After 12 weeks, participants from PCG presents lower body weight, relative fat mass and higher free fat mass (p < 0.001 for all) compared to GC. QOL improved among adolescents from both groups (p < 0.05), however, a better QOL was reported from those adolescents enrolled in PCG. Conclusion: The inclusion of a psychological counseling component in multidisciplinary treatment for adolescent obesity appears to provide benefits observed for improved QOL as compared with treatment without psychological counseling.

Resumo Objetivo: Investigar os efeitos do tratamento multidisciplinar com e sem aconselhamento psicológico voltado para a qualidade de vida de adolescentes obesos. Métodos: Foram alocados 76 adolescentes obesos (15,87 ± 1,53 ano) em um grupo de aconselhamento psicológico (GAP; n = 36) e um grupo de controle (GC; n = 40) por 12 semanas. Todos receberam o mesmo treinamento físico supervisionado e aconselhamento nutricional e clínico. Os participantes no GAP também receberam aconselhamento psicológico. A qualidade de vida foi avaliada antes e depois das 12 semanas de intervenção por meio do Questionário Genérico de Avaliação da Qualidade de Vida (SF-36). Resultados: O abandono do tratamento foi maior no GC (22,5%) em comparação com o GAP (0,0%) (p < 0,001). Após 12 semanas, os participantes do GAP apresentam menor peso corporal, massa gorda relativa e maior massa livre de gordura (p < 0,001 para todos) em comparação com o GC. A qualidade de vida melhorou entre os adolescentes de ambos os grupos (p < 0,05); contudo, uma melhor qualidade de vida foi relatada pelos adolescentes incluídos no GAP. Conclusão: A inclusão de aconselhamento psicológico no tratamento multidisciplinar dos adolescentes obesos parece proporcionar benefícios observados na melhoria da qualidade de vida, em comparação com o tratamento sem aconselhamento psicológico.

Efficacy of bleomycin for non-operative treatment of cervical lymphangioma in University of Ilorin Teaching Hospital, Nigeria

Background: Lymphangiomas are the developmental defects of the lymphatic channels, and they are most commonly found in the head and neck regions. Late presentation, rejection of surgery, and traditional scarification result in fatal complications. Surgical excision often thought to give immediate relief and aesthetic results is associated with damage to contiguous structures and recurrence, hence, the need for less invasive treatment modality. Objective: To assess the effectiveness of bleomycin sclerotherapy of cervical lymphangiomas. Materials and Methods: This is a prospective study of patients with cervical lymphangioma treated with sclerosant injection between January 2008 and December 2016. Preinjection ultrasound scan and initial ultrasound-guided aspiration of the fluid in the swelling (which many times is multiloculated) using a 20G cannula into a 10 ml syringe were performed. The cannula tip is retained in the space and intralesional injection of double-diluted bleomycin 0.5 i.u./kg body weight was given as outpatient at 2­4-weekly interval. Postinjection events were documented. The clinical assessment of the pre- and postinjection of sclerosant was performed. Result: A total of 23 patients were recruited, and six were females and 17 were males. All swellings were noticed at birth but median time at presentation was 17 days. All patients but one (95.8%) had complete clinical resolution after 1­4 courses of sclerotherapy for 4­16 weeks. Only one patient had residual nodule that required surgical excision. Redundant skin and hyperpigmentation from skin wrinkle were the early effects noticed in three patients; however, these were cosmetically acceptable to the parents. No recurrence was recorded. Conclusion: The treatment of cervical lymphangiomas with intralesional bleomycin injection is shown to be effective. It is safe and associated with no complication. This treatment modality and outcome was found to be acceptable to the parents of these children

Customer Attitudes to Water Use and Its Conservation.

Aims: Understanding how customers engage with and view their water usage is crucial to the design of more effective water demand management policies and programmes. This paper presents the findings of a small-scale research project that sought to explore customer attitudes to the use of water and its conservation, particularly in the context of seasonal tariffs used during the summer peak usage months (May to August). Study Design: The study adopted a qualitative approach, implemented through a series of faceto- face semi-structured interviews. Place and Duration of Study: The study was conducted with domestic water users in Bishops Stortford, East Hertfordshire (UK). The research was carried out by staff from the School of Life and Medical Sciences at the University Hertfordshire. The study was conducted over a period of 6 weeks. Methodology: A series of 20 face-to-face semi-structured interviews were carried out with a predefined sample population. The selected customers were split into two equal sized groups depending on their relative water usage to reflect either an increase or a decrease in water usage [as a consequence of Affinity Water’s seasonal tariff trial]. Results and Discussion: The study highlights study participants as being disengaged from their water usage and the associated efforts to reduce their usage, so simply increasing water prices at seasonal peak usage times was not, on this occasion, an effective method to adopt to reduce domestic water usage. However, by subsequently exploring customer attitudes towards a selected range of alternative water conservation measures, such as the subsidisation of water efficient appliances, and rebates for reduced water usage, it is established that alternative water conservation measures may have the potential to more effectively encourage a reduction in water usage. However, as the findings of this study also serve to highlight, the issue of ‘institutional trust emerges as a key issue to consider when seeking reductions in water usage by increasing its unit cost, with accusation of profiteering looming large. Conclusion: It is suggested that a richer mix of policy responses demand management will be needed to convince domestic water users of the need to reduce their water usage.

Malarial Morbidity and Postnatal HIV Infection in Breastfeeding HIV-exposed Infants.

Background: For at-risk HIV-negative individuals, whether malarial morbidity increases the likelihood of HIV infection when exposed is unknown. Hence, we investigate the malaria-associated risk of postnatal HIV infection in 1804 breastfeeding infants of HIV-positive women from Dar es Salaam, Tanzania. Methods: Six-week-old HIV-negative infants were followed until breastfeeding cessation or postnatal HIV infection. HIV-1 status was determined by a DNA PCR test. Malarial morbidity was diagnosed by physicians using a combination of clinical symptoms and laboratory tests. For analytic purposes, malaria was distinguished by diagnostic specificity as: (1) clinical; (2) probable, where laboratory testing is requested for parasitemia; and (3) blood smear-confirmed. Hazard ratios (HR) and 95% confidence intervals (CI) for the risk of HIV infection were estimated from multivariate Cox regression models. Results: Mean follow-up duration was 6.2 months (standard deviation=2.4 months), during which 91 new HIV infections developed and clinical malaria was diagnosed in 594(32.3%) children, including 283 (15.5%) probable and 80(4.4%) confirmed malaria episodes. Infants ever diagnosed with clinical and probable malaria were at 73% (95%CI:1.11 - 2.69) and 100% (95%CI:1.17-3.42) higher risk of postnatal HIV infection, respectively. This risk increased by 39% (95%CI: 1.08-1.80) and 59% (95%CI: 1.00-2.32), respectively, per episode increment in clinical and probable malarial; however, confirmed malaria was not significantly associated with HIV incidence (HR=2.09; 95%CI: 0.74 - 5.91). Conclusion: We found positive associations between child malarial infection and postnatal HIV infection among breastfeeding HIV-negative children of HIV-positive women. These findings suggest that malaria prevention in such infants may decrease the risk of HIV mother-to-child-transmission. However, specific future studies using laboratory-confirmed malaria in HIV-negative but HIV at risk populations are needed to substantiate these findings.

Reasons and Barriers to Lose Weight: Obese Adolescents’ Point of View.

Aims: To identify the reasons why obese adolescents seek weight loss therapy and what barriers they face in losing weight. Study Design: Cross-sectional study. Place and Duration of Study: Department of Physical Education and School Medical Science, University of Pernambuco 2009-11. Methodology: The study was conducted with 128 obese adolescents (76 girls [BMI=35.46±3.92] and 52 boys [BMI=33.70±2.88]), aged between 12 to 18 years old Personal reasons for seeking weight loss treatment and barriers to losing weight were obtained by an individual semi-structured interview (8 questions) conducted by a psychologist. All individual interviews lasted approximately 20 min and were performed in a quiet room. Adolescents’ answers were semi-transcribed by the researcher and the content was anonymously analyzed in order to categorize the data. Results: The main reason for girls to start a weight loss program was to become healthy (39.47%), followed by to fit in clothes (30.26%), personal appearance (30.26%), and bullying (28.95%). Physical fitness (40.38%) was the most important reason to seek weight loss for boys, followed by to become healthy (36.54%), and bullying (25%). For both genders, the main barriers described were lack of self-control (47.37% - 36.54%), lack of social support (27.63% - 30.77%), and self-motivation (22.37% - 21.15%). Conclusion: Obese adolescents had several reasons for seeing weight loss. Overall, becoming healthy was the main motivation for weight loss and lack of self-control was the main barrier. This information can be used to help develop more effective weight loss strategies for obese adolescents.

Robot-assisted vs. Laparoscopic Partial Nephrectomy: utilization rates and perioperative outcomes

Objectives To examine the effect of surgical approach on perioperative morbidity and mortality after partial nephrectomy. Materials and Methods Within the Nationwide Inpatient Sample, patients who underwent RAPN or LPN between October 2008 and December 2009 were identified. Propensity-based matching was performed to adjust for potential baseline differences between the two groups. The rates of intraoperative and postoperative complications, blood transfusions, prolonged length of stay, and in-hospital mortality, stratified according to RAPN vs. LPN, were compared. Results Overall, 851 (72.5%) patients underwent RAPN and 323 (27.5%) underwent LPN. For RAPN and LPN respectively, the following rates were recorded in the propensity-score matched cohort: blood transfusions, 4.5 vs. 6.8% (p = 0.223); intraoperative complications, 5.2 vs. 2.6% (p = 0.096); postoperative complications, 10.6 vs. 13.5% (p = 0.268); prolonged length of stay, 6.8 vs. 9.4% (p = 0.238); in-hospital mortality, 0.0 vs. 0.0%. Conclusions RAPN has supplanted LPN as the predominant minimally invasive surgical approach for renal masses. Perioperative outcomes after RAPN and LPN are comparable. Interpretation of these findings needs to take into account the lack of adjustment for case complexity and surgical expertise. .

Evaluation of haemodynamic changes in hypertensive patients during tooth extraction under local anaesthesia / Evaluación de los cambios hemodinámicos en pacientes hipertensos durante la extracción dental con anestesia local

OBJECTIVES: This study was conducted to determine the changes in blood pressure and the pulse rate of patients with controlled hypertension having dental extraction under local anaesthesia utilizing 2% lignocaine with adrenaline, and to evaluate whether these changes in blood pressure were are attributable to addition of adrenaline. METHODS: This prospective study was carried out in 33 consecutive hypertensive patients who presented at the exodontia clinic of the Department of Oral and Maxillofacial Surgery, Lagos University Teaching Hospital (LUTH), Idi-Araba, Lagos, from December 2004 to August 2005 for intra-alveolar tooth extraction. Patients were randomly allocated to two groups according to the type of anaesthetic solution employed. Group A had tooth extraction done under 2% lignocaine with 1:80 000 adrenaline while group B had tooth extraction done under 2% lignocaine without vasoconstrictor (plain lignocaine). One tooth was extracted from each patient. Blood pressure and pulse rate measurements were recorded in the waiting room before surgery, in the surgery after local anaesthetic injection, during tooth extraction and 15 minutes after tooth extraction. RESULTS: The sample consisted of 20 females and 13 males age range 24 to 75 years (mean ± SD = 50.1 ± 11.7 years). There was no statistically significant difference between the systolic and diastolic blood pressure and pulse rate in the two groups after administration oflocal anaesthesia. However, the highest alteration in parameters was observed during tooth extraction in the two groups. CONCLUSION: The haemodynamic changes induced by injecting 2% lignocaine with adrenaline in patients with controlled hypertension during tooth extraction is within normal range and is not different from that induced by 2% lignocaine without adrenaline. We consider it essential that all precautions to prevent inadvertent intravascular injection be undertaken by the care provider.

OBJETIVO: Este estudio fue dirigido para determinar los cambios en la tensión arterial y la tasa de pulso de pacientes con hipertensión controlada, a quienes se les realiza una extracción dental bajo las condiciones de la anestesia local, utilizando lidocaína al 2% con adrenalina, y evaluar si estos cambios en la tensión arterial eran atribuibles a la adición de adrenalina. MÉTODO: Este estudio prospectivo se llevó a cabo en 33 pacientes hipertensos consecutivos que acudieron a la clínica de exodoncia del Departamento de Cirugía Oral y Maxilofacial del Hospital Docente Universitario (LUTH), Idi-Araba, Lagos, desde diciembre 2004 a agosto 2005, para extracciones intra-alveolares. Los pacientes fueron colocados de forma aleatoria en dos grupos, según el tipo de solución anestésica empleada. Al grupo A se le realizó la extracción con lidocaína al 2% con 1:80 000 adrenalina, mientras que al grupo B se le realizó la extracción con lidocaína al 2% sin vasoconstrictor (lidocaína pura). A cada paciente se le extrajo una pieza. Mediciones de la presión arterial y el pulso fueron realizadas en el salón de espera antes dela cirugía, en la cirugía tras la inyección con la anestesia local, durante la extracción del diente, y 15 minutos después de la extracción. RESULTADOS: La muestra consistió en 20 hembras y 13 varones cuyo rango de edad fluctuó de 24 a 75 años (± SD media = 50.1 ± 11.7 años). No hubo diferencia estadísticamente significativa alguna entre la tensión arterial sistólica y la diastólica, ni con respecto a la tasa del pulso en los dos grupos luego de la administración de la anestesia local. Sin embargo, la alteración más alta de los parámetros se observó durante la extracción en los dos grupos. CONCLUSIÓN: Los cambios hemodinámicos inducidos por la inyección de lidocaína al 2% con adrenalina en los pacientes con hipertensión controlada durante la extracción, están dentro del rango normal y no son distintos de los inducidos mediante lidocaína al 2% sin adrenalina. Se considera fundamental que los profesionales de la salud tomen todas las precauciones para prevenir inyecciones intravasculares inadvertidas.

The bowel habits of adolescents in Nigeria.

Background: The implications of defaecation patterns to health are important. The abnormalities can be readily recognized and treated only if the normal pattern is known. Methods: This cross sectional study involved 1115 adolescents enrolled using a stratified multi-stage random sampling technique. A self-administered and semi-structured questionnaire was used to obtain information on stool frequency, volume, colour and consistency. Data were compared between low and high across socioeconomic strata. Results: There were 481 males (overall mean age: 15.2+1.9 years). Participants were on mixed carbohydrate rich diet with both high and low fibre content.. Stool frequency was at least once daily in 77.3%, once in alternate days (17.4%) and once in >3days (5.3%). There was no association between stool frequency and gender (p=0.141) or age (p=0.345). More participants from the high (64.9%) than the low (35.1%) social classes opened their bowels once in >3days (p=0.048). More males produced larger stool volumes (>200ml) than females, (p=0.000). More than half (50.5%) of the participants reported passing brownish stool, 46.3% yellow stool and 3.2% greenish brown stool. Passage of soft stool was reported by 74.9% of the participants. Overall, 3.4% of participants had passed blood in their stool at some time. Conclusion: Majority of Nigerian adolescents passed 25-200 ml of soft stool atleast once daily and age and gender did not affect stool frequency. High socioeconomic status was associated with passage of hard stool and less frequent bowel opening. This data could facilitate early recognition of deviation from normal bowel habits in Nigerian adolescents.

Biosafety education relevant to genetically engineered crops for academic and non-academic stakeholders in East Africa

Development and deployment of genetically engineered crops requires effective environmental and food safety assessment capacity. In-country expertise is needed to make locally appropriate decisions. In April 2007, biosafety and biotechnology scientists, regulators, educators, and communicators from Kenya, Tanzania, and Uganda, met to examine the status and needs of biosafety training and educational programs in East Africa. Workshop participants emphasized the importance of developing biosafety capacity within their countries and regionally. Key recommendations included identification of key biosafety curricular components for university students; collaboration among institutions and countries; development of informational materials for non-academic stakeholders and media; and organization of study tours for decision makers. It was emphasized that biosafety knowledge is important for all aspects of environmental health, food safety, and human and animal hygiene. Thus, development of biosafety expertise, policies and procedures can be a stepping stone to facilitate improved biosafety for all aspects of society and the environment.

Bilateral opening in the nasal bones of goats in Nigeria

The present study was performed to observe the morphological details of the skulls of the major goat breeds in Nigeria involving 28 Red Sokoto, 24 Sahel and 12 West African Dwarf goats. The study revealed the presence of a bilateral opening in the nasal bones of the skulls. This unique occurrence appeared in about 70 of the Red Sokoto, less than 10 of the West African Dwarf and did not occur in the Sahel goat skulls. It was suggested that the structure which displayed a high level of breed specificity may be contributing to the weakness of the nasal area of the face. This finding to the best of our knowledge is rarely described in the literature and will be important in breed identification of goats and can contribute to the migratory history of the species.

Designing quality control for neonatal screening assays.

The purpose of neonatal screening is to find those that have a high risk for a disorder and therefore need further action for diagnosis and treatment. The separation of the high-risk and low-risk groups is typically achieved by establishing cut-off values for interpretation of the test result. The guidelines should preferably include both a low cut-off and a high cut-off value, with a grey zone between them. The width of the grey zone can be used for defining the quality required by the assay. The present work is based on the use of the EZ Rules software (from Westgard QC, Madison, WI), which automatically selects control rules. The user enters the needed parameters, such as the precision (as % CV) and bias of the assay. By using the grey zone as the medical decision interval the program will calculate possible control rules for the assay. The program was used to calculate the control rule for the AutoDELFIA neoTSH assay (from PerkinElmer Wallac, Turku, Finland). The grey zone was taken as 10 - 20 mU/L TSH, which is the recommendation of the American Academy of Pediatrics (Pediat. 91, 1203 - 1209). The entered parameters were: a total imprecision of 9%, which is typically seen with the AutoDELFIA neoTSH kit, a bias of 0% and a preanalytical variation of 20%. With the number of controls chosen as two, as often is used, a 1 3.0 s rule can be applied. The program also gives alternative control rules. Many laboratories lack a documented definition of the required quality, and tend to use a 2 SD control rule, which however leads to many unnecessary rejections. The EZ Rules program provides a tool for selection of QC rules. With the quality of the AutoDELFIA neoTSH kit two controls and a 1 3.0 s rule is sufficient. Runs are rejected only if one control out of two exceeds the 3.0 SD limit.

Prevalence of reactive serological tests for syphilis in the Jamaican population

Serological tests for syphillis (STS) were performed on sera from 15,876 members of the general adult population, 3,039 pregnent women and 1,029 children, in order to assess the prevalence and value of reactive STS inJamaicans; 2.7% of sera from the general adult population, 4.7% from pregnant women and 2.2% from children were reactive in the Venereal Disease Research Laboratory (VDRL) test. Reactive Flourescent Treponemal Absorption (FTA-ABS) tests occurred in significantly higher frequency in pregnant women (4%) than in the general population (2.2%; p<0.001). The prevalence of reactive STS in children didnot differ significantly from that of the general adult population. The predictive value of a reactive VDRL test in pregnant women (85%) was higher than that of the general population (79%). These results suggest that inadequate screening with the inexpensive VDRL test would enable the early detection and treatment ofsyphilis, and could prevent increasing incidence of congenital syphilis in Jamaica