ABSTRACT
OBJECTIVE: To establish the HPLC specific chromatogram of Oldenlandia corymbosa Linn. and Oldenlan diadiffusa (Willd.) Roxb. in different regions so as to distinguish these two traditional Chinese medicinal materials. METHODS: HPLC was performed on an Agilent Eclipse XDB-C18(4.6 mm×250 mm,5 μm) column with mobile phase consisting of methanol-water at a flow rate of 1 mL·min-1, column temperature at 30℃ and detection wavelength at 254 nm. Similarity evaluation system for chromatographic fingerprint of TCM was used to conduct similarity evaluation and Matlab7.0 software was used for principal component and cluster analysis. RESULTS: Oldenlandia corymbosa Linn. and Oldenlan diadiffusa (Willd.) Roxb. had eight common peaks, namely, a-h; the characteristic peaks of O. corymbosa were 7 to 12, while the characteristic peaks of O. diffusa were 1 to 6. The similarity values of O. corymbosa and O. diffusa collected from different sources were 0.733-0.984 and 0.873-0.951, respectively. According to the principal components analysis and cluster analysis, the tested samples were classified into three categories: O. corymbosa collected from wild, O. diffusa collected from wild and purchased O. diffusa. CONCLUSION: The established method is reliable and rapid to distinguish the two kinds of easily confused traditional Chinese medicinal materials and also can offer reference for their quality control and clinical use.
ABSTRACT
To establish the fingerprints of Xiasangju granules (with sugar and non-sugar forms) by HPLC, and provide reference for their identification and effective quality control. High performance liquid chromatography (HPLC) method was used to collect the fingerprints of 20 batches of non-sugar Xiasangju granules and 34 batches of sugar type Xiasangju granules. Their main different components were classified and screened by mode identification methods (principal component analysis, PCA, and orthogonal partial least squares discriminate analysis, OPLS-DA). The principal components were identified by comparing with reference standards. The fingerprints of Xiasangju granules (sugar type and non-sugar type) were established. PCA could not fully classify the two types of granules, while OPLS-DA could obviously classify these two different types of Xiasangju granules. Six components showed greatest difference between two types of granules, including salviaflaside, luteoloside and linarin. The developed mode identification method is helpful to control the overall quality of Xiasangju granules, and it provides an effective approach to quality evaluation.
ABSTRACT
This paper was aim to screen microorganisms with attenualed efficiency for Chinese medicine containing aristolochic acid A by liquid-state fermentation. Twelve Chinese medicine were detected by UPLC and aristolochic acid A was only founded in four species of Aristolochia, those were Caulis Aristolochiae Manshuriensis, Aristolochiae Radix, Aistolochia Contorta Bunge and Herba Aristolochiae Mollissima,but not in the others. With the four Chinese medicine containing aristolochic acid A as raw material, ten microorganisms were tested, and the content of aristolochic acid A was detected by UPLC. The results showed that one microorganism can decrease content of aristolochic acid A in all those four Chinese medicine.