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Objective To explore whether or not the function of atragalus membranaceus combined fosinopril on decreasing NF- κB activity and OPN expression in overload albumin stimulating proximal tubuler cells. Methods Cultured cells without stimulation were used as placebo. Cultured cells were incubated with bovine serum albumin fat acid free (BSA) 20mg/ml as the control. Cultured cells were incubated with fosinopril for different hours as the treatment. Cultured cells were incubated with atragalus membranaceus combined fosinopril for different hours as the atragalus membranaceus group. ELISA and RT-PCR were used to observe NF -κB activity and OPN expression after cultured for 12h, 24h. Result Atragalus membranaceus combined fosinopril rapidly decreased NFκB activity and OPN expression. Compared with the other groups, the difference was significant. Conclusion Astragalus membranaceus combined fosinopril can obviously decrease NFκB activity and OPN expression in overload albumine stimulating proximal tubular cells.
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Objective To investigate the regulation of cyclooxygenase-2 expression and mechanism of selective cyclooxygenase-2 inhibitor Celecoxib suppressing tubulointerstitial fibrosis of rats with unilateral ureteral obstruction(UUO) model. Methods The UUO models were induced by ligating the left ureter. Rats were randomly divided into bulofen group (group B), Celecoxib group (group R), model group (group C) and sham operation group (group S). Rats in group B were given bulofen 300mg?Kg~ -1?d~ -1, and in group R were given Celecoxib 10mg?Kg~ -1?d~ -1 by gastric gavage from 24 h before the obstruction to 14 days after the induction. Rats were sacrificed at 3d, 6d and 14d in batch after the UUO models were induced. The mRNA expressions of cyclooxygenase-1(COX-1), cyclooxygenase-2(COX-2), transforming growth factor-beta 1 (TGF-?1), and connective tissue growth factor (CTGF) were detected by RT-PCR. The protein expressions of TGF-?1, CTGF and ?-SMA were detected using immunohistochemistry and the content of cAMP was determined by radioimmuno-assay. Results Compared with group S, the mRNA expressions of COX-2, TGF-?1 and CTGF in group C increased markedly after UUO treatment, and the content of cAMP decreased. It showed no significant difference in the mRNA expressions of TGF-?1, CTGF and the content of cAMP for the Bulofen treatment in the UUO model. With the Celecoxib treatment, there was no significant difference on the mRNA expression of TGF-?1, but the content of cAMP increased and the expression of CTGF decreased. Conclusion The COX-2 plays an important role in lesion of tubulointerstitial. Selective cyclooxygenase-2 inhibitor can partially up-regulate the content of cAMP and down-regulate the expression of CTGF, the downstream factor of TGF-?1, which may postpone the renal interstitial fibrosis.
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Objective To study the molecular mechanism of the apoptosis in rat tubular epithelial cells induced by albumin in vitro.Methods The cultured rat renal tubular epithelial cells were incubated with 20 mg/ml delipidated and endotoxin-free bovine serum albumin(BSA) for 2,6,12 and 24 h respectively.The NF-?B activity was assessed by electrophonetic mobility shift assay(EMSA) and the osteopontin(OPN) mRNA expression was analyzed by reverse transcription-polymerase chain reaction(RT-PCR).The cell apoptosis was evaluated by flow cytometry.Results The rate of apoptosis was increased when the cultured rat renal tubular epithelial cells were incubated with 20 mg/ml BSA for 2,6,12 and 24 h respectively.The NF-?B activity and expression of OPN were up-regulated in the cultured rat renal tubular epithelial cells with the treatment of BSA,with significant difference compared with those of the control group(P
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Objective To explore the effects of fluvastatin on NF-?B activity and osteopontin(OPN) mRNA expression in albumin-induced renal tubular epithelial cells.Methods The renal tubular epithelial cells were cultured with 30 mg/ml fat free bovine serum albumin(BSA) as the control group.The renal tubular epithelial cells in the treatment group were cultured with different concentrations of fluvastatin for different hours.EMSA and RT-PCR were used to observe NF-?B activity and OPNmRNA expression.Results Fluvastatin can inhibit the NF-?B activity and OPNmRNA expression in a time and dose dependent manner.Conclusion Fluvastatin can inhibit NF-?B activity and OPNmRNA expression in albumin induced tubular epithelial cells.
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Objective To investigate the effect of EPO on T lymphocyte subgroup and cytokine in hemodialysis patients. Methods Thirty-two cases of patients underwent long-term hemodialysis were as experimental group, and 20 healthy subjects were as control group. The levels of CD 3, CD 4, CD 8 and CD 4/CD 8 were examined by flow cytometry in the both groups before and 3 months after EPO treatment, and the levels of SIL-2R, IL-6 and TNF? were detected by ELISA. Results CD 4 level significantly increased 3 months after EPO treatment (P
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Objective To investigate expression of connective tissue growth factor(CTGF) in the kidney of patients with type 2 diabetic mellitus(DM) as well as its clinical significance.Methods Fourty cases with DM were divided into three groups: normal albuminuric group(200 ?g/min,n=12).The urinary excretion rates of CTGF were determined by ELISA in all the cases and 30 subjects of control.Twenty cases of them received renal biopsy.Expression of CTGF in kidney among different groups were detected by immunohistochemical stainning.Results Expression of CTGF in kidney elevated significantly in DM as compared with group of normal control.At the same time the excretion rates of CTGF in DM groups were markedly higher than that in control(P