ABSTRACT
Objective To establish detection method of glinides adulterated illegally in hypoglycemic traditional Chinese medi -cine (TCM) by surface enhanced Raman spectroscopy (SERS).Methods Adulterated chemicals and TCM matrixes were separated by TLC first.Then trace substances in TLC plate was tested by SERS method .By investigating SERS detection conditions of glinides chemi-cals in simulated positive samples , a detection method was established to detect illegal adulterant in hypoglycemic TCM .Results Better SERS spectra of glinides could be obtained by silver sol prepared with organic solvent DMF .Conclusion The detection method coupled with TLC and SERS in this paper was simple , fast and economical which could be used to detect glinides adulterated illegally in hypogly -cemic Chinese patent medicine quickly .