ABSTRACT
In this paper, the effect of asolectin liposome on the activity of rabbit skeletal muscle lactate dehydrogenase (LDH) is reported. The results suggested that asolectin liposome could inhibit LDH activity, KC1 could restore the enzyme activity, and NAD+ and NADH could protect the enzyme from being inhibited by asolectin liposome.
ABSTRACT
This report deals with the comparative study on the vitellogenesis in autogenous and anautogenous strains of Aedes togoi. Histochemical and immunohistochemical methods were used. It was found that rule of vitellogenesis is characteristic in different strains within the same species. Synthesis of vitellogenin in autogenous strain begins as soon as post-emergence and the process goes on continuously, up to the oocytes to take in vitellin for maturation. Vitellogenin synthesis in anautogenous strain starts after blood meal, and there is an apparent testing stage before blood feeding. The capacity of vitellogenin synthesis is also different between two strains.
ABSTRACT
The ovary development in autogenous and anautogenous strains of Aedes togoi was stud led.Under the laboratory conditions, autogenous and anautogenous strains were selected from the wild caught Aedes togoi, and different stages of ovary development were observed.The experiment demonstrated that the biological characteristics of the ovary development in these two strains were evidently different, such as the size and number of matured follicles, the continuity and the pace of developing pro:esses.The main cause for the differences is the time and amount of vitellogenin deposited in oocytes.
ABSTRACT
In this paper, we studied the kinetics of nitochondrial lactate dehydrogenase (LDH) and solubilized mitochondrial LDH in the rat liver. The apparent Km values of mitochondrial LDH and solubilized mitochondrial LDH for substrate pyruvate were 50.0 ?mol/L and 33.8 ?mol/L, and those for NADH were 35.3?mol/L and 21.4 ?mol/L, respectively. The apparent Km values of mitochondrial LDH were greater than those of solubilized nitochondrial LDH. The mitochondrial LDH in the rat liver was mainly LDH-5, which could be solubilized by 0.15 mol/L NaQ solution.