A new triterpenic acid from the roots of Rosa laevigata / 药学学报

This study was designed to investigate triterpenoids from the roots of Rosa laevigata Michx. The silica gel column chromatography was used to separate the chemical constituents from the roots of Rosa laevigata Michx. HPLC was used to analyze its purity and chemical constitution. Spectroscopy methods were used to determine their structures. Five constituents were isolated and identified as19α-OH-3β-E-feruloyl corosolic acid (1), 23-hydroxy-tormentic acid (2), 2α, 3β, 19α, 23-tetrahydroxy-12-en-28-oleanolic acid (3), 2α, 3α, 20β-trihydroxyurs-13 (18)-en-28-oic-acid (4), 2α, 3β, 20β-trihydroxyurs-13 (18)-en-28-oic-acid (5). Compound 1 was assigned as a new compound, compounds 4, 5 were obtained from the genus Rosa for the first time.

Determination of linarin in Yuye Jiedu granule by RP-HPLC / 中国中药杂志

<p><b>OBJECTIVE</b>To determine the content of linarin in Yuye Jiedu granule.</p><p><b>METHOD</b>A HPLC method was developed. The chromatographic conditions are as follows Luna C18 column and acetonitrile-0.05 mol x L(-1) phosphate buffer-phosphoric acid (30:70:0.06) as mobil phase, detection wavelenth at 327 nm.</p><p><b>RESULT</b>The linear range of linarin was 0.025-0.50 microg. The average recovery was 98.7% and RSD 2.7%.</p><p><b>CONCLUSION</b>The method is simple and accurate, with good repeatability, and can be used for determination of linarin in Yuye Jiedu granule.</p>

A new picfeltarraenone glycoside from Picria fel-terrae / 药学学报

<p><b>AIM</b>To investigate the chemical constituents from Picria fel-terrae Lour.</p><p><b>METHODS</b>Column chromatography techniques were used to isolate the chemical constituents, physico-chemical constants and spectroscopic analysis were employed for structural elucidation. Results Two triterpenoids named picfeltarraenone I (1) and picfeltarraenin XI (2) were isolated, and their structures were established to be 3,11,22-trioxo-16alpha-hydroxy-(20S,24)-epoxy-cucurbit-5,23-diene (1) and 3,11,22-trioxo-16alpha-hydroxy-(20S,24)-epoxy-cucurbit-5, 23-diene-2beta-O-beta-D-glucopyranoside (2), respectively.</p><p><b>CONCLUSION</b>Compound 2 is a new compound, the 13CNMR data of compound 1 is reported for the first</p>

Effects of Naomaitai capsule on learning and memory and content of Ach in rat model produced by cerebral ischemia-reperfusion / 中国中药杂志

<p><b>OBJECTIVE</b>To observe the effects of Naomaitai capsule on the learning and memory abilities and the content of acetylcholine(Ach) in the brain tissue of the model rat.</p><p><b>METHOD</b>One vascular dementia(VD) model was produced by occlusion of bilateral common carotid arteries in rats with following steps: ischemia 20 minutes-reperfusion 10 minutes-ischemia 20 minutes. The learning and memory abilities were tested by water maze. The other VD model was formed by 4-vessel occlusion-reperfusion injury in rats. The time of right reflect recovery and the content of Ach in the brain tissue were determined.</p><p><b>RESULT</b>Naomaitai capsule can significantly improve the learning and memory abilities of the dementia rats( P < 0.05, P < 0.01), increase the content of Ach (P < 0.05, P < 0.01) and reduce the time of right reflect recovery(P < 0.01).</p><p><b>CONCLUSION</b>Naomaitai capsule can improve the learning and memory abilities by increasing the content of Ach of the VD rats.</p>

Study on the chemical constituents from Picria fel-tarrae / 中国中药杂志

<p><b>OBJECTIVE</b>To isolate and identify compounds from Picria fel-tarrae in order to utilize it better.</p><p><b>METHOD</b>Constituents from Picria fel-tarrae were isolated by several column chromatography and their structures were elucidated on the basis of chemical and spectral analysis.</p><p><b>RESULT</b>Six compounds, N-benzoylphenylalanyl-L-phenylalaninol acetate (1), 1-hydroxy-7-hydroxymethyl-9,10-anthraquinone (2), 9, 16-dioxo-10,12,14-octadeca-trienoic acid (3), 5,7,4'-trihydroxy-flavone (4), beta-sitosterol (5), and daucosterol (6) were obtained from the fraction with relatively low polarity of EtOH extract of Picria fel-tarrae.</p><p><b>CONCLUSION</b>Compounds 1-6 were isolated from picria fel-tarrae Lour for the first time, and the 13C-NMR data of compounds 1-3 are provided firstly in the literature.</p>

Isolation and identification of a new cucurbitacin from Picria fel-terrae / 药学学报

<p><b>AIM</b>To study compounds isolated from Picria fel-terrae.</p><p><b>METHODS</b>The chemical constituents were separated and purified by column chromatography on silica gel and MCI. Their structures were identified on the basis of spectral data (IR, UV, MS, ID NMR and 2D NMR).</p><p><b>RESULTS</b>A new cucurbitacin, along with a known one, were obtained from the 60% EtOH extract of the whole plant.</p><p><b>CONCLUSION</b>The new compound was identified as 11, 24-dioxo-5, 21-diene-cucurbit-3alpha-O-beta-D-xylopyranosyl-16alpha-O-alpha-L-rhamnopyranoside (dehydrobryogenin glycoside). The known one, hexanorcucurbitacin F, was obtained for the first time from Picria fel-terrae.</p>

Resonance Rayleigh scattering spectra of berberine-tetraphenylboron association nanoparticle and its analytical application / 药学学报

<p><b>AIM</b>To study the relationship between resonance Rayleigh scattering(RRS) and the formation of association nanoparticle, and to develop a new and sensitive RRS method for the determination of trace berberine (BB).</p><p><b>METHODS</b>The association nanoparticle between BB and tetraphenylboron (TPB) was investigated by means of RRS, absorption spectral method and transmission electron microscope (TEM).</p><p><b>RESULTS</b>In pH 5.0 NaAc-HAc buffer solution, BB and TPB combine to BB-TPB association complex. The association complexes aggregate to (BB-TPB)n association nanoparticles, due to the strong hydrophobic force and Van der Waals force. It exhibits a resonance Rayleigh scattering peak at 470 nm, a maximum absorption peak at 368 nm. A new RRS method was proposed for the determination of 0.06 to 5.28 mg.L-1 BB in real samples with satisfactory results. The detection limit is 26 micrograms.L-1.</p><p><b>CONCLUSION</b>The TEM of (BBjAgp-TPBj + p)h composite association nanoparticles was observed by means of composite association nanoreaction both TPB- and BB+ or Ag+. The results indicated that the formation of (BB-TPB)n association nanoparticles and interface of solid-liquid results in the enhanced resonance light-scattering. This new RRS method showed high sensitivity, and had been successfully applied to the determination of BB in real samples.</p>

Kinetic study on the volatility of menthol and borneol / 中国中药杂志

<p><b>OBJECTIVE</b>To study the volatile regularity of menthol and borneol in granule and lozenge at different temperature.</p><p><b>METHOD</b>Kinetic method with GC being detection technique.</p><p><b>RESULT</b>The volatility of menthol and borneol acts as a pseudo first-order reaction in open system. Under the same condition, the volatile rate of menthol and borneol in granule is four times as fast as that in lozenge, and the volatile rate of borneol is faster than that of menthol.</p><p><b>CONCLUSION</b>This study can be applied to improve the quality of lozenges containing menthol or/and borneol.</p>