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Objective To investigate the clinical therapeutic effect of citicoline sodium combined with Shenxiong glucose in the treatment of senile hypertensive cerebral infarction in the elders.Methods 80 elderly patients with senile hypertensive cerebral infarctiontreated in our hospital were selected and randomly divided into the single treatment group and the combination treatment group,40 cases in each group.Both groups received the routine treatment.The single treatment group additionally received Shenxiong glucose injection (100 ml/d),while patients in the combination treatment group additionally received Shenqiong glucose injection combined with citicoline sodium intravenous infusion therapy (0.5 g/d),both groups were treated for 2 weeks.The levels of serum inflammatory factors,the neurological deficit score,the cognitive function score were compared and analyzed before and after treatment between two groups.Results After systemic treatment,the blood pressure and blood lipid levels of two groups were significantly improved,but there was no significant difference between the two groups (P ＞ 0.05);The serum levels of interleukin (IL)-6,IL-8 and tumor necrosis factor-α (TNF-α) of the combination treatment group improved more significantly (P ≤0.05).After treatment,the oxidative stress indexes were significantly improved in the two groups (P ≤ 0.05).The content of malondialdehyde (MDA) was decreased,while the superoxide dismutase (SOD) activity and nitric oxide (NO) content were increased significantly (P ≤ 0.05);and the improvement degree in the combination treatment group was better than in the single treatment group (P ≤0.05).The degree of improvement in the Modified Edinburgh-Scandinavia Stroke Scale (MESSS) and Hasegawa Dementia Scale (HDS) scores of the combination treatment group was more significant than those in the single treatment group (P ≤ 0.05).The total effective rate of the combination treatment group was 92.5％,which was significantly higher than that of the single group (75.0％),with statistically significant difference (P ≤ 0.05).No obvious adverse reactions happened in two groups during treatment.Conclusions Combination of citicoline sodium and shenxiang glucose on the basis of routine treatment can significantly reduce oxidative stress and inflammation levels,promote the recovery of neurological and cognitive functions,and improve the clinical efficacy and safety.It is worth popularizing and applying in the clinical treatment of senile hypertensive cerebral infarction.
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Objective:To explore trend of congenital heart disease (CHD) ,analyze its epidemiological features and risk factors .Methods :A total of 2415 children diagnosed as CHD in our hospital from Jan 2012 to Jan 2015 were en‐rolled as CHD group ,another .Detection rate of every year ,constitution type and constituent ratio of each age group for CHD were counted .Multifactor Logistic regression analysis was used to analyze independent risk factors and protective factors of CHD .Results:CHD detection rate of 2012 ,2013 and 2014 was 19.42% ,34.99% and 45.59% respectively ,showing an increasing trend (P=0.018 ,0.107) .The top three types of CHD were atrial sep‐tal defect (ASD , 37.31% ) , ventricular septal defect (VSD , 30.97% ) and patent ductus arteriosus (PDA , 13.71% ) .Comparison among all age groups found that 1 year children ;< 4 years CHD children occupied 74. 95% for all CHD children .Multifactor Logistic regression analysis indicated that educa‐tion level of mother was independent protective factor for CHD [OR=0.488 ,P=0.011] ,threatened abortion his‐tory ,smoking history of father and interior renovation during pregnancy were independent risk factors for CHD [OR=1.748~7.151 ,P<0.05 or <0.01] .Conclusion:Detection rate of congenital heart disease shows a gradual increasing trend in recent years ,periodic inspection and early intervention focus on parents status and environmental factors must be performed in order to achieve goals of early diagnosis ,prevention and treatment .
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Objective To screen and clone the genes related to stilbene glucosides biosynthesis in Polygonum multiflorum Thunb. Methods The differentially expressed genes in the root, stem and leaf of Polygonum multiflorum which have different contents of stilbene glucosides were screened by differential display reverse transcription polymerase chain reaction (DDRT-PCR). After pMD19-T carrier was inserted into the obtained differential genes for sequencing and comparison, the gene function was analyzed. Results Fifty-one differentially expressed cDNA fragments were found. Of them, 9 were used for the identification by semi-quantitative PCR. The identification results presented 3 positive fragments, one fragment was specifically expressed in the stem and leaf of Polygon-um multiflorum Thunb., sharing high homology with glycine dehydrogenase, and 2 were specifically expressed in the root of Polygonum multiflorum Thunb., having high homology with enoyl-CoA hydratase and aminopeptidase N, respectively. Conclusion Three homologous gene sequences obtained through DDRT-PCR provide a basis for the further study of biosynthetic pathway of stilbene glucoside from Polygonum multiflorum Thunb..