ABSTRACT
This study was purposed to investigate the relationship between expression of the FANCG gene and adult sporadic acute myeloid leukemia (AML), real-time PCR with SYBR Green I technique was used for detecting FANCG gene expression level in bone marrow mononuclear cells of 54 newly diagnosed AML patients, 46 AML patients in complete remission (CR) and 36 control samples. β-actin gene was used as internal reference. Relative changes of FANCG gene expression level were detected by 2(-ΔΔCT) method in newly diagnosed AML patients and control samples, in newly diagnosed AML and patient in CR, as well as in AML patients in CR and control samples. The results showed that the relative expression level of FANCG mRNA was 0.56 ± 0.27 in newly diagnosed group, 0.75 ± 0.54 in AML CR group, and 0.85 ± 0.45 in control group. The expression level of FANCG mRNA in newly diagnosed group was significantly lower than that in control and AML CR groups (P < 0.05). There was no statistically significant deference in comparison of AML CR group with the control group (P > 0.05). It is concluded that the expression of FANCG gene decrease in the newly diagnosed AML patients. There is no significant difference between AML CR group and control group, which indicated that FANCG gene may be related with the onset and the prognosis of AML, and may provide a clinical value for evaluating effect of chemotherapy.
Subject(s)
Adult , Female , Humans , Male , Middle Aged , Fanconi Anemia Complementation Group G Protein , Genetics , Leukemia, Myeloid, Acute , Genetics , Pathology , Prognosis , RNA, Messenger , Genetics , Real-Time Polymerase Chain ReactionABSTRACT
<p><b>OBJECTIVE</b>To study the effects of Bushen Yin' ao Tablet (BSYNT) on physiology and cerebral gene expression in senescence-accelerated mice (SAM).</p><p><b>METHODS</b>The change of cerebral tissues mRNA expression in SAM was analyzed and compared by messenger ribonucleic acids reverse transcription differential display polymerase chain reaction (mRNA DDRT-PCR) between the medicated group and the control group.</p><p><b>RESULTS</b>BSYNT could increase the level of hemoglobin (Hb) and amount of erythrocyte (RBC) of blood deficiency mice, improve the spatial learning and memory function and the escape response by conditional stimulus. In this study, 14 differential display bands had been discerned, and three of them had been sequenced. The sequence of the three fragments was similar to fatty acid binding protein 7, ubiquinol-cytochrome C reductase complex (7. 2 kD) and 60S ribosomal protein L21 respectively. And the homogeneity was 97% , 100% , and 99% , respectively.</p><p><b>CONCLUSION</b>BSYNT has effect on the physiological changing of mice, and its effect on cerebral tissues mRNA expression maybe play an important role in anti-aging on the molecular level.</p>
Subject(s)
Animals , Mice , Aging , Brain , Metabolism , Drugs, Chinese Herbal , Pharmacology , Electron Transport Complex III , Genetics , Metabolism , Erythrocyte Count , Fatty Acid-Binding Protein 7 , Fatty Acid-Binding Proteins , Genetics , Metabolism , Gene Expression , Hemoglobins , Metabolism , Nerve Tissue Proteins , Genetics , Metabolism , RNA, Messenger , Metabolism , Ribosomal Proteins , Genetics , Metabolism , Spatial Behavior , TabletsABSTRACT
<p><b>OBJECTIVE</b>To explore multi-drug resistance (MDR) of bladder cancer for the intravesical instillation.</p><p><b>METHODS</b>Using immunohistochemical staining, in 44-case human bladder cancer cells, the expressions of P-glycoprotein (P-gp), glutathione S-transferase (GST-pi) and topoisomerase (TOPO-II), were detected to find out the resistance to drugs.</p><p><b>RESULTS</b>P-gp had a higher expression in 54.5% cases. GST-pi had no or a lower expression in 65.9% cases. TOPO-II had a higher expression in 29.5% but a lower expression in 65.9% cases.</p><p><b>CONCLUSION</b>Detecting the factors of MDR in bladder cancer cells could help to choose drugs for intravesical chemotherapy.</p>