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China Journal of Chinese Materia Medica ; (24): 2092-2095, 2013.
Article in Chinese | WPRIM | ID: wpr-346436

ABSTRACT

<p><b>OBJECTIVE</b>To establish a rapid, sensitive and efficient detection method for tobacco mosaic virus (TMV), and provide technical support of TMV detection of Rehmannia glutinosa f. hueichingensis. The virus-free plantlets could be produced on a large scale to ameliorate breed degeneration caused by viral disease.</p><p><b>METHOD</b>Specific primers were designed based on the conserved region of coat protein(CP) gene of TMV. Immunocapture RT-PCR (IC-RT-PCR) was employed to detect TMV and the sequence of the products was detected.</p><p><b>RESULT</b>The expected nucleotide acid fragments were amplified by IC-RT-PCR. The homology of nucleotide acid sequence and amino acid sequence were 95.29% and 96.7% between the PCR products and the CP gene of TMV (accession number AY555269).</p><p><b>CONCLUSION</b>The method was established for the detection of TMV in R. glutinosa f. hueichingensis by IC-RT-PCR. This detection combined molecular biology technology with immunology, was convenient for a quick, sensitive and simple detection of TMV.</p>


Subject(s)
Amino Acid Sequence , Base Sequence , Molecular Sequence Data , Rehmannia , Virology , Reverse Transcriptase Polymerase Chain Reaction , Methods , Tobacco Mosaic Virus , Genetics , Allergy and Immunology
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