ABSTRACT
The authors regret for the changes in Fig. 6 as follow: [Figure presented] Fig. 6. HPLC-ELSD chromatograms of ten Anoectochilus, four Goodyera and one Ludisia species on AQ-C
ABSTRACT
<p><b>OBJECTIVE</b>To provide DNA molecular marker for identification of Nelumbo nucifera by exploring the differences of nrDNA-ITS sequence of N. nucifera originated from different habitats.</p><p><b>METHOD</b>To compare nrDNA-ITS base sequence using specific PCR-ITS.</p><p><b>RESULT</b>The completed sequence of ITS and 5.8 S rDNA, and the partial sequences of 18S rDNA and 26S rDNA, totally 750 bp, from N. nucifera were obtained. The differences among N. nucifera from different habitats and from different cultivars were found.</p><p><b>CONCLUSION</b>The method can be used to identify N. nucifera among different species and to distinguish their fakes. It provided the basis for identifying N. nucifera from different geographical regions by comparison of their ITS sequences.</p>
Subject(s)
Base Sequence , China , DNA, Plant , Chemistry , Genetics , Metabolism , DNA, Ribosomal Spacer , Classification , Genetics , Deoxyribonuclease EcoRI , Metabolism , Deoxyribonucleases, Type II Site-Specific , Metabolism , Drug Contamination , Geography , Nelumbo , Classification , Genetics , Phylogeny , Plants, Medicinal , Classification , Genetics , Polymerase Chain Reaction , RNA, Ribosomal , Genetics , RNA, Ribosomal, 18S , Genetics , Genetics , Sequence Analysis, DNA , Species SpecificityABSTRACT
<p><b>OBJECTIVE</b>To investigate the clinicopathologic characteristics and prognosis of multiple primary synchronous colorectal carcinoma (synchronous CRC) and single colorectal carcinoma (single CRC).</p><p><b>METHODS</b>Clinicopathological data of 39 patients with synchronous CRC and 528 patients with single CRC from May 1996 to June 2001 were reviewed retrospectively.</p><p><b>RESULTS</b>The patients with synchronous CRC accounted for 6.9% of all the patients with colorectal carcinoma treated in our hospital during the same period. Compared with concurrent lesions, Dukes stage of the index lesions of synchronous CRC was more developed and the lymph node metastasis and vessel invasion occurred more frequently. In addition, it also had poorer differentiation. There were significant differences of Dukes stages and lymph node metastasis between the index lesions and single CRC. The incidence of adenomatous polyp in synchronous CRC was higher than that in single CRC(59.0% vs 25.0%,P<0.01). The sensitivity of preoperative colonoscopy examination was 76.9%, which was significantly higher than that of barium examination and exploration during operation. The total 5-year survival rate of synchronous CRC was significantly lower than that of single CRC (5.1% vs 28.2%,P=0.042), and no significant difference of total 5-year survival rate was found between single CRC patients and synchronous CRC patients undergone radical operation. Synchronous CRC patients undergone radical operation had longer survival as compared to those undergone palliative operation(P<0.01). Multivariate analysis indicated that Dukes stages, lymph node metastasis, vessel invasion and the type of operation were independent prognostic factors of synchronous CRC.</p><p><b>CONCLUSIONS</b>Synchronous CRC and single CRC are not always similar in clinicopathologic characteristics and prognosis. Early finding, early diagnosis and radical operation are the keys to improve the survival rate of synchronous CRC.</p>
Subject(s)
Aged , Female , Humans , Male , Middle Aged , Colorectal Neoplasms , Pathology , Lymphatic Metastasis , Neoplasm Staging , Neoplasms, Multiple Primary , Pathology , Prognosis , Retrospective Studies , Survival RateABSTRACT
<p><b>OBJECTIVE</b>To promote its comprehensive utilization, the involatile constituents of Mentha haplocalyx were studied systematically.</p><p><b>METHOD</b>The chemical components were isolated and purified by silca gel column chromatography and recrystallization. The chemical structures were elucidated on the basis of physico-chemical properties and spectral data.</p><p><b>RESULT</b>Eight compounds were isolated and identified as: acacetin (I), tilianine (II), linarin (III), n-butyl-beta-D-fructopyranoside (IV), ursolic acid (V), oleanolic acid (VI), beta-sitosterol (VII), daucosterol (VIII).</p><p><b>CONCLUSION</b>Compounds I approximately V were obtained from this plant for the first time.</p>