ABSTRACT
OBJECTIVE@#To study the expression of Survivin, Caspase-3 and p53 in surgical margin of laryngeal squamous cell carcinoma (LSCC), and their clinical significance and relationship.@*METHOD@#Immunohistochemistry method was used to detect the expression of Survivin, Caspase-3 and p53 in 116 cases of surgical margin of LSCC.@*RESULT@#According to the recurrence, the patients were divided into four group, recurrence within one year, between one year to three years, between three to five years, more than 5 years (include no recurrence). The expression rate of survivin were 92.9%, 85.0%, 50.0% and 21.1% (P < 0.01) in four groups, respectively. The expression rate of Caspase-3 were 50.0%, 50.0%, 66.7% and 89.5% (P < 0.01). The expression rate of p53 were 57.1%, 37.5%, 45.8% and 18.4% (P < 0.05). The expression rate of survivin, caspase-3 and p53 in four groups, respectively, there were significant differences (P < 0.05).@*CONCLUSION@#Expression of Survivin, Caspase-3 and p53 in surgical margin of LSCC may play an important role in the tumorigenesis and development of laryngeal carcinoma. They are feasible for early diagnosis and prognosis estimation.
Subject(s)
Aged , Female , Humans , Male , Middle Aged , Carcinoma, Squamous Cell , Metabolism , Pathology , General Surgery , Caspase 3 , Metabolism , Inhibitor of Apoptosis Proteins , Metabolism , Laryngeal Neoplasms , Metabolism , Pathology , General Surgery , Neoplasm Recurrence, Local , Prognosis , Survivin , Tumor Suppressor Protein p53 , MetabolismABSTRACT
OBJECTIVE@#To study the effect of IgE, GM-CSF on eosinophil accumulation in the nasal polyps and find the relationship between them.@*METHOD@#Thirty mucosa constitution samples of nasal polyps and eleven inferior nasal concha were observed. The distribution of IgE was detected by SP immunohistochemical method, while the concentration of GM-CSF was measured by ELISA technique and the number of eosinophils were counted in the same tissues.@*RESULT@#The level of IgE, GM-CSF and eosinophils had significant different between the nasal polyps and control group (P < 0.01). There was positive correlation between IgE/GM-CSF and the number of eosinophils in the nasal polyps group (r = 0.65, 0.62, P < 0.01). The correlation between concentration of IgE and GM-CSF was also significant in nasal polyps group (r = 0.52, P < 0.01).@*CONCLUSION@#Nasal polyps is characterized by eosinophil accumulation. IgE and GM-CSF may contribute to the recruitment of eosinophils in nasal polyps. Type-I allergy mediated by IgE play an important role in the pathogenesis of nasal polyps.