ABSTRACT
The obstacles and risks of the application of imaging devices such as CT,PET/CT,MRI and in vivo imaging system in animal biosafety level 3/4(ABSL-3/4)laboratories were proposed.Combined with the experience of applying imaging devices in ABSL-3/4 laboratories,the key issues were discussed such as application scheme,risk assessment and control,disinfection and sterilization methods.Some suggestions were put forward for applying large-scale imaging equipment in ABSL-3/4 laboratories.References were provided for ABSL-3/4 laboratories equipped with imaging devices.[Chinese Medical Equipment Journal,2023,44(9):69-73]
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of oral carcinoma-associated fibroblasts (CAFs) on extracellular signal-regulated kinases (ERK) pathway in a lingual carcinoma cell line.</p><p><b>METHODS</b>The lingual carcinoma cell line, Tca8113 was stimulated by conditioned medium from oral CAFs, or cocultured with oral CAFs for definite time. Total ERK and pERK in Tca8113 lysate were detected by Western blotting, and the ratio between pERK and ERK were calculated.</p><p><b>RESULTS</b>Both stimulation by conditioned medium and coculture induced prompt phosphorylation of ERK, and increased the ratio between pERK and ERK.</p><p><b>CONCLUSION</b>Oral CAFs can activate ERK pathway of carcinoma cells.</p>
Subject(s)
Humans , Cell Line , Cell Line, Tumor , Coculture Techniques , Extracellular Signal-Regulated MAP Kinases , Fibroblasts , Mouth Neoplasms , Phosphorylation , Tongue NeoplasmsABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of oral carcinoma-associated fibroblasts (CAFs) on the invasion of a lingual carcinoma cell line, and to elucidate the role of CAFs in oral squamous cell carcinoma (OSCC) progression.</p><p><b>METHODS</b>Matrigel was used to remodel the basement memberane, and the interaction model between primary oral CAFs and lingual carcinoma cell line Tca8113 was established by Transwell chamber to observe the effect of CAFs on the invasion of Tca8113.</p><p><b>RESULTS</b>Compared with normal fibroblasts (NFs), CAFs promoted more Tca8113 cells to penetrate Matrigel (P < 0.05).</p><p><b>CONCLUSION</b>Oral CAFs can promote the invasion of lingual carcinoma cell line Tca8113, and play a key role in OSCC progression.</p>
Subject(s)
Humans , Carcinoma, Squamous Cell , Cell Line , Cell Line, Tumor , Cell Proliferation , Fibroblasts , Mouth Neoplasms , Tongue NeoplasmsABSTRACT
<p><b>OBJECTIVE</b>To adopt a criterion summarized in long-term practice to judge the immune status of recurrent aphthous ulceration (RAU) patients, and to analyze its rationality and feasibility.</p><p><b>METHODS</b>60 cases were included, whose clinical information, such as age, sex, constitution status, history of system diseases and severity of ulceration were collected. The subjective judgement results of the immune status were obtained according to the criterion summarized in clinical practice. At the same time, peripheral blood parameters about immune function were examined in order to obtain the objective examination results of the immune status. With the examination result as the gold standard, the rationality of the criterion was estimated through its sensitivity and specificity.</p><p><b>RESULTS</b>According to subjective judgment, there were 86.67% cases with abnormal immune status (28.33% cases with elevated immune status, and 58.33% cases with reduced immune status). According to objective examination, there were 93.33% cases with abnormal immune status (23.33% cases with elevated immune status, and 70.00% cases with reduced immune status. Sensitivity and specificity were 89.29% and 50.00% when the criterion was used to judge immune abnormality, 42.86% and 76.09% when the criterion was used to judge immune elevation, and 64.29% and 55.56% when the criterion was used to judge immune reduction.</p><p><b>CONCLUSION</b>It is of some rationality and feasibility to judge the immune status according to the criterion based on clinical information, and the criterion can be used in small clinics.</p>