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Objective To evaluate the effect of epigallocatechin gallate on radiation-induced lung injury in rats and to discuss the related mechanism. Methods Rat models of radiation-induced lung injury were produced by 6 MV X ray radiation, and then the animals were divided into model control group and low-, medium- and high-dose (200, 100, 50 mg/ [kg • d]) epigallocatechin gallate groups. The pulmonary histopathological changes and collagen disposition were observed by H-E staining and Van Gieson (VG) staining, and the scores of alveolitis and pulmonary fibrosis were calculated. The expression of tumor necrosis factor-α (TNFα) and transforming growth factor β ( TGFβ ) in lung tissues was examined by immunohistochemical (IHC) staining. The activity of superoxide dismutase (SOD) was examined by xanthine oxidase method. Results Apparent alveolitis and pulmonary fibrosis were observed in model rats at 40 d after radiation, and the scores of the alveolitis and pulmonary fibrosis were signficantly lower in medium- and high-dose epigallocatechin gallate groups than in the model control group (P<0. 01), and there was no difference between the low-dose epigallocatechin gallate group and the model control group. Pulmonary TNFα and TGFβ expression was significantly lower in the medium- andhigh-dose epigallocatechin gallate groups than in the model control group and low-dose epigallocatechin gallate groups at 10 d, 20 d, and 40 d after radiation (P<0. 01 or P<0.05), while SOD activity in the former two groups were significantly higher compared with those in the model control group and low-dose epigallocatechin gallate groups ( P < 0. 05). Conclusion Medium- and high-dose.
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<p><b>OBJECTIVE</b>To identify the major components of traditional Chinese medicine Naodesheng tablet.</p><p><b>METHODS</b>A HPLC-DAD-MS(n) based method was developed to analyze and identify the major components of Naodesheng tablet. Separation was performed on an Agilent Zorbax SB-C(18) column (4.6 mm X 250 mm, i.d, 5 μm) with mobile phase consisting of water with 0.05 % formic acid and acetonitrile as gradient eluent at the flow rate of 0.5 ml.min(-1).</p><p><b>RESULTS</b>A total of 43 components were detected, among which 22 were identified by comparing their UV absorption profiles, the information of molecular Glucosyl puerarin weights, and structures provided by ESI-MS(n) with those of available standards and reference data, such as Safflor yellow A, 4'-O-Glucosyl puerarin, 3'-hydroxypuerarin, Genistein-8-C-apiosyl (1-6) glucoside, Puerarin, 6"-O-xylosyl puerarin, 6"-O-apiosyl puerarin, 3'-methoxy puerarin, 3'-methoxy-6"-o-xylosyl puerarin, Daidzin, Genistin, Pueroside A, Notoginsenoside R(1), Ginsenoside Re, Ginsenoside Rg1,Daidzein,Biochanin A,Ginsenoside Rb(1), Ginsenoside Rc, Ginsenoside Rb(2), Ginsenoside Rb(3), Ginsenoside Rd.</p><p><b>CONCLUSION</b>The proposed method can identify the main components of Naodesheng tablet and provide information for the quality control of this medicine.</p>
Subject(s)
Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Chemistry , Ginsenosides , Isoflavones , Mass Spectrometry , MethodsABSTRACT
Objective: To compare the efficacy and adverse reactions between gemcitabine + cisplatin regimen and fluorouracil + cisplatin regimen in the treatment of advanced nasopharyngeal carcinoma (NPC). Methods: The Cochrane Library,. MEDLINE, EMbase, CBM and CNKI were searched for related articles published before 2009. Manual searching and additional searching were also performed. The quality of each included studies was evaluated by 2 independent oncologists and analyses of homogeneous property of the studies were done by RevMan 5.0 software. Results: Four studies were enrolled in our study, including 283 patients with NPC. Meta-analyses showed that the 1-year- and 3-year-survival rates of gemcitabine + cisplatin regimen were similar to those of fluorouracil+cisplatin regimen (Z = 0.89, P = 0.37), (Z = 0.70, P = 0.09), but gemcitabine+ cisplatin regimen had significantly higher remission rate (Z = 2. 34, P = 0.02) and slighter myelosuppression and gastrointestinal reactions (Z = 2.47, P = 0.01), (Z = 3. 20, P = 0.001), (Z = 2.19, P = 0.03). Conclusion: Gemcitabine +cisplatin regimen has equivalent efficacy and slighter adverse effects compared with fluorouracil + cisplatin regimen in treatment of advanced nasopharyngeal carcinoma.