ABSTRACT
Alzheimer's disease （AD） is the most common form of senile dementia， and its pathogenesis is still unclear. While β-amyloid （Aβ） is considered an important cause of AD， the pathological mechanism of Aβ inducing AD is subject to various controversies. Recent studies have shown that the myeloid cell trigger receptor （TREM2） plays an important role in the pathological process of AD， and it can not only serve as an important receptor for the internalization of Aβ but also become a biological diagnostic biomarker and therapeutic target. Hence， elucidating the structure and function of TREM2 will provide important ideas for the prevention and treatment of AD. This article will provide a systematic review of the structure of TREM2， its impact on microglial cell function， its pathological role in AD， and the current status of targeted TREM2 therapy for AD. These summaries will provide valuable references for basic research on AD.
ABSTRACT
OBJECTIVE@#To determine the apoptosis-inducing effect of ultraviolet light (UV) on human lens epithelial cell (HLEC) and to explore the involvement of changes in ALDH1 folowing UV radiation.@*METHODS@#HLEC was exposed to the same UV light source and was subsequently divided into 6 groups according to UV radiation time of 0 (control group), 5, 10, 15, and 30 min. Apoptosis was detected by AO/EB staining. Changes of ALDH1 in HLEC were detected by immunohistochemical staining and Western blot.@*RESULTS@#The intensity of immunohistochemical staining and the rate of positive cells decreased with increase of UV time (P<0.05). The rate of positive ALDH1 cells was negatively correlated with the rate of apoptosis (r= -0.92, P<0.05). Western blot showed the integrated absorbance values significantly decreased with the increase of UV time (P<0.05).@*CONCLUSION@#ALDH1 in HLEC decreases with an increase of UV exposure, which may be related to UV induced apoptosis of HLEC.
Subject(s)
Humans , Aldehyde Dehydrogenase 1 Family , Apoptosis , Radiation Effects , Cells, Cultured , Epithelial Cells , Cell Biology , Metabolism , Radiation Effects , Isoenzymes , Genetics , Metabolism , Lens, Crystalline , Cell Biology , Retinal Dehydrogenase , Genetics , Metabolism , Ultraviolet RaysABSTRACT
OBJECTIVE@#To explore the apoptosis-inducing effect of ultraviolet(UV) radiation on human lens epithelial cells (HLEC), with particular focus on changes in Bcl-2 or Bax expression as possible mechanisms.@*METHODS@#All experimental groups were exposed to the same UV light source. HLEC were divided into 6 groups according to duration of UV radiation : 0 min group (control group), 5 min group, 10 min group,15 min group, and 30 min group. Analysis on apoptosis of HLEC was performed by flow cytometry analysis (FCA, Annexin V + PI staining). Changes of Bax and Bcl-2 expression in HLEC were detected by hybridization in situ.@*RESULTS@#Apoptosis in HLEC increased with UV exposure time. The expression level of Bax mRNA was increased with the increase of UV exposure time, whereas the expression level of Bcl-2 mRNA decreased with the increase of UV exposure time. The proportion of apoptotic cells was negatively correlated with ratio of Bcl-2/Bax (r=-0.874, P<0.05).@*CONCLUSION@#UA radiation can induce apoptosis of HLEC in vitro. Bcl-2 and Bax genes may play an important role in regulating this apoptotic process.