ABSTRACT
The cDNA fragment of JEV prME gene was cloned into the baculovirus shuttle vector (bacmid) to construct a recombinant baculovirus vector, defined as AcBac-prME. Then the recombinant baculovirus Ac-prME was obtained by transfecting Sf9 cells with AcBac-prME. Western blot analysis and immunofluorescence results indicated that both prM and E proteins were efficiently expressed in Sf9 cells. Electron microscopy suggested that prME was assembled into JEV-VLPs. To further evaluate the potential of JEV-VLPs as vaccine, the mice were immunized with JEV-VLPs and then challenged with lethal JEV. The results of mice survival and pathological changes demonstrated that the JEV-VLPs performed complete protection against JEV-P3 strain and relieved pathological changes in the mice brain significant. This study suggest that JEV-VLPs would be a potential vaccine for Japanese encephalitis virus.
Subject(s)
Animals , Humans , Mice , Antibodies, Viral , Allergy and Immunology , Encephalitis Virus, Japanese , Genetics , Allergy and Immunology , Encephalitis, Japanese , Allergy and Immunology , Virology , Japanese Encephalitis Vaccines , Genetics , Allergy and Immunology , Mice, Inbred BALB C , Sf9 Cells , Vaccination , Vaccines, Virus-Like Particle , Genetics , Allergy and Immunology , Viral Envelope Proteins , Genetics , Allergy and ImmunologyABSTRACT
<p><b>OBJECTIVE</b>To study the indications, methods and experience of absorbable rib-connecting-pins fixation in the treatment of multiple rib fractures.</p><p><b>METHOD</b>52 cases with multiple rib fractures were performed internal fixation with absorbable rib-connecting-pins under epidural anesthesia.</p><p><b>RESULTS</b>All cases were followed up for 1 to 12 months, with an average of 5 months. All fractures were achieved healing in 3 to 6 months after the operation and were not found chest wall deformity.</p><p><b>CONCLUSIONS</b>Absorbable rib-connecting-pins fixation is a simple and effective method and worthies recommending to perform operation for the appropriate cases with multiple rib fractures.</p>