ABSTRACT
<p><b>OBJECTIVE</b>To establish a method to determine the underivatized glycine (Gly) and proline (Pro) in vinegar turtle shell.</p><p><b>METHOD</b>An HPLC-ELSD method was conducted on a Prevail C18 column (4.6 mm x 250 mm, 5 microm) with the mobile phase of acetonitrile and 0.7% trifluoroacetic acid solution (containing 5.0 mmol x L(-1) heptafluorobutyric acid), and elution time was 15 min.</p><p><b>RESULT</b>The calibration curves were showed good linearity within the concentration range of 0.14-0.6 g x L(-1). The average recoveries were 101.2% and 102.5%, and RSD were 1.9% and 2.5%, respectively.</p><p><b>CONCLUSION</b>Since this method needs neither the special amino acid analyzer nor derivation of amino acid., it is efficient, simple and accurate., which could be used for quality control of vinegar turtle shell.</p>