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OBJECTIVE To investigate the correlation between vascular endothelial function and aortic compliance in patients with obstructive sleep apnea hypopnea syndrome(OSAHS) and hypertension. METHODS A total of 103 OSAHS cases were divided into simple OSAHS group(n=55) and OSAHS complicated with hypertension group(n=48, OSAHS+HT group). 20 patients with simple hypertension were included into the hypertension group, and 20 healthy people served as controls. Vascular endothelial function was evaluated by detecting flow mediated dilation of the brachial artery(FMD) with color Doppler; the changes of aortic compliance in patients were detected by echocardiography. RESULTS The FMD, aortic tension and aortic distensibility index levels of OSAHS with hypertension group were significantly lower than the normal control group, hypertension group and OSAHS group(P<0.05); The aortic stiffness index level of OSAHS hypertension group was significantly higher than the normal group, the high blood pressure group and OSAHS group(P<0.05); Analysis of Spearman correlation coefficient of patients in OSAHS with hypertension group found aortic FMD were positively correlated with aortic stiffness index, negatively correlated with aortic tension index and aortic expansion; The aortic FMD, aortic tension, swelling index and aortic stiffness index in OSAHS hypertension group had positive correlation with AHI (P<0.05), and negative correlation with LSaO2(P<0.05). CONCLUSION OSAHS is associated with vascular endothelial dysfunction and decreased aortic compliance. When combined with hypertension, vascular endothelial function and aortic compliance will be significantly affected. The severity of OSAHS was closely related to the decrease of vascular endothelial function and the changes of aortic compliance.
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Objective To observe the clinical effect of adenoidectomy in the treatment of children with chronic rhinosinusitis ,to discuss its postoperative complications and postoperative rehabilitation .Methods Chose 100 children with chronic rhinosinusitis from February 2011 to April 2013 treated in our hospital as observation objects ,the cases were randomly divided into experimental group and control group ,with 50 cases in each group ,patients in experimental group was given drug and adenoidectomy treatment ;patients in control group was given drug orally external treatment only .Observed the postoperative complications and postoperative recovery ,then compared treatment effect of two groups .Results The total efficiency of experimental group was 96% ,control group was 60% ,the difference was statistically significant (P<0 .05);compared with those before operation ,there were significant differ-ence in experiment group in postoperative snoring ,purulence stuff ,sinus patency ,etc(P<0 .05) .Moreover ,experimental group and control group had difference in hospitalization days .The control group not only had high cost and long hospital stay but also had relatively high recurrence rate (P<0 .05) .Conclusion The curative effect of adenoidectomy combined with drugs in the treatment of children with chronic rhinosinusitis was much better than drug treatment only .
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A case of 76-years-old male patient with nasal bleeding as the first symptom in our hospital, who was finally diagnosed as tsutsugamushi disease. This old man was bited by insect in farmland 2 days before the symptom occurred. PE: Left thigh and right buttock have eschar, with splenomegaly. Routine blood test: WBC (decrease) 3.9 x 10(9)/L, RBC (decrease) 3.86 x 10(9)/L, PLT (decrease) 41 x 10(9)/L, HGB (decrease) 117 g/L; Chest CT: lung interstitial pneumonia, a small amount of bilateral pleural effusion. Oxk-ag 1:320. The patient was discharged after treatment with chloramphenicol for 8 days.
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Aged , Humans , Male , Epistaxis , Diagnosis , Scrub Typhus , DiagnosisABSTRACT
OBJECTIVE@#To study the expression of VEGF in sinonasal squamous cell carcinoma and its correlations with microvessel density (MVD), microlymphatic vessel density (MLVD).@*METHOD@#The expression of VEGF, MVD and MLVD in 41 cases of sinonasal squamous cell carcinoma were detected by immunohistochemical technique.@*RESULT@#In the sinonasal squamous cell carcinoma, the positive rate of VEGF was 82.9% (34/41). The over expression of VEGF was related with tumor invasion, histological grading and lymphatic metastasis (P 0.05).@*CONCLUSION@#VEGF may participate in the metastasis of sinonasal squamous cell carcinoma through promoting vascularization in the tumors, but not promoting MLVD.
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Female , Humans , Male , Carcinoma, Squamous Cell , Metabolism , Pathology , Microvessels , Nasal Cavity , Neovascularization, Pathologic , Nose Neoplasms , Metabolism , Pathology , Paranasal Sinus Neoplasms , Metabolism , Pathology , Vascular Endothelial Growth Factor A , MetabolismABSTRACT
OBJECTIVE@#To explore the eye complication of nasopharyngeal carcinoma (NPC), to analysis the clinical manifestation, CT characteristics and pathological diagnosis of eye complications of NPC and to provide the base for early diagnosis of NPC.@*METHOD@#To retrospectively study of 82 cases eye complications in 562 cases NPC, to study their clinical manifestation, CT characteristics and pathological diagnosis.@*RESULT@#The clinical studies showed that eye complication cases were occurred in 82 cases of 562 NPC cases (14.6%). Thirty-six cases in left and 37 cases in right eye, 9 cases in bilateral eyes. Sixty-five cases came from Guangdong, while the others 17 cases come from 5 provinces. There were 9 kinds of eye manifestation. CT appearances: 40 cases suffered from skull base distracted, 6 cases with orbit involved, 2 cases ( in left eyes) with orbit metastasis, 12 cases with nose-sinus involved, 68 case with parapharyngeal space involved, 49 cases with soft issue in wall of styloid process involved (there were many kind of shows in the same case, so the data were repeated in these cases).@*CONCLUSION@#There were multiplicity and complexity in eye complication of NPC. Ophthalmologists should think highly of these cases.
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Adult , Aged , Female , Humans , Male , Middle Aged , Blindness , Diagnosis , Carcinoma , Diplopia , Diagnosis , Nasopharyngeal Carcinoma , Nasopharyngeal Neoplasms , Diagnosis , Pathology , Neoplasm Metastasis , Retrospective Studies , Tomography, X-Ray ComputedABSTRACT
OBJECTIVE@#To study the pathological relationship between the expression of Col IV and LN in nasal and paranasal sinus malignant tumor (NPMT).@*METHOD@#The immunohistochemical technique was used to detected the expression of Col IV and LN in NPMT, para-cancer tissues and non-cancer tissues.@*RESULT@#There was a significance on the expression of the Col IV and LN in NPMT, para-cancer tissues and non-cancer tissues (P0.05).@*CONCLUSION@#The Col IV and LN perhaps participate in tumorigenesis of NPMT, and may play the homoioplastic role in different pathological types of the NPMT.
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Adolescent , Adult , Aged , Aged, 80 and over , Child , Female , Humans , Male , Middle Aged , Young Adult , Collagen Type IV , Metabolism , Laminin , Metabolism , Nasal Cavity , Pathology , Nose Neoplasms , Metabolism , Pathology , Paranasal Sinus Neoplasms , Metabolism , PathologyABSTRACT
OBJECTIVE@#To investigate the inhibition of hTERT gene to Hep-2 cells of laryngeal neoplasms.@*METHOD@#siRNA-hTERT mRNA was designed. Hep-2 cells, cultured in vitro, were transfected with lipofectamine 2000 and the inhibitory effect was detected by reverse transcriptase (RT-PCR), MTT assay, and morphology.@*RESULT@#1. siRNA were transfected into Hep-2 cells successfully; 2. RT-PCR showed the exogenous hTERT was knocked down 70% by siRNA; 3. The morphology and the MTT result showed the hTERT siRNA to be able effectively to suppress the Hep-2 cell multiplication, the suppression rate achieves 65%.@*CONCLUSION@#siRNA target to hTERT can remarkably suppress the proliferation of Hep-2 cell.
Subject(s)
Humans , Carcinoma, Squamous Cell , Genetics , Cell Line, Tumor , Gene Targeting , Genetic Vectors , Laryngeal Neoplasms , Genetics , RNA , Genetics , RNA, Messenger , Genetics , RNA, Small Interfering , Telomerase , Genetics , TransfectionABSTRACT
OBJECTIVE@#To investigate the effect of small interfering RNA (siRNA) targeting c-myc gene in Hep-2 cells.@*METHOD@#siRNA targeting c-myc mRNA was designed and synthesized. In vitro cultured Hep-2 cells were transfected with lipofectamine 2000 and the inhibitory effect was detected by MTT, morphology, real time PCR assay.@*RESULT@#1) The MTT result showed the c-myc siRNA to be able effectively to suppress the Hep-2 cell multiplication; 2) The real time PCR result showed c-myc at mRNA level inhibition ratio at 94% in group S3; 3) The morphology result showed the c-myc siRNA to be able effectively to suppress the Hep-2 cell multiplication, the cell heteromorphism was diminished.@*CONCLUSION@#siRNA targeting c-myc can remarkably suppress the Hep-2 cell growth and multiplication.
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Humans , Apoptosis , Cell Division , Cell Line, Tumor , Cell Proliferation , Gene Targeting , Genes, myc , Genetics , RNA, Small Interfering , Genetics , TransfectionABSTRACT
OBJECTIVE@#To study the clinical significance of the expression of p53, p63 and p73 protein and the correlation of p53 and p63 in nasal and paranasal sinus carcinoma (NPSC).@*METHOD@#The immunohistochemical technique was used to detect the expression of p53, p63 and p73 in 67 cases of NPSC, para-cancer tissues and non-cancer tissues.@*RESULT@#The positive rate of p53 and p63 protein in NPSC was significantly higher than that in park cancer tissues and non-cancer tissues (P0.05).@*CONCLUSION@#There are positive correlation between p53 and p63 protein in NPSC, and they may play an important role in the tumorgenesis of NPSC. The p73 may not be associated with tumorgenesis of NPSC.
Subject(s)
Female , Humans , Male , Middle Aged , Carcinoma, Squamous Cell , Metabolism , Pathology , DNA-Binding Proteins , Metabolism , Nose Neoplasms , Metabolism , Pathology , Nuclear Proteins , Metabolism , Paranasal Sinus Neoplasms , Metabolism , Parasitology , Trans-Activators , Metabolism , Transcription Factors , Tumor Protein p73 , Tumor Suppressor Protein p53 , Metabolism , Tumor Suppressor Proteins , MetabolismABSTRACT
OBJECTIVE To investigate the rela-tionship between the expression of the proto-oncogene cerbB-2 proteins p185, level of adhesion molecules CD44v6, and lymphoid metastasis of laryngeal squa-mous cell carcinomas (LSCC). METHODS The expres-sion of p185 and CD44v6 in 63 specimen of LSCC tissues were examined by immunohistochemical method. RESULTS There was a significant difference in the expression of p185 between patient groups with and without lymphoid metastasis(P
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Objective:In order to ascertain the lateral operation and medial maxillectomy affect on nasal airway.Method:Report 16 cases of nasal cavity or nasal sinus neoplasm who were therapied by lateral rhinotomy and medial maxillectomy.They were observed by acoustic rhinometer (AR) and CT examination in 1～2.5 years after the surgery. Result:The feeling of nasal obstruct were disappeared in 3 months after the surgery. The nasal airway resistance(NAR) of the ill nasal cavity was markedly decreased after the surgery, and the total NAR were decreasing unmarkably. The mucous proliferation was notable only in the first year after surgery, and especially on remains of the inferior turbinate or extra wall of nasal cavity.Conclusion:Lateral rhinotomy operation and medial maxillectomy have less impact on nasal airway and the functions.
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AIM:To explore the effects of small interfering RNA(siRNA) targeting PCNA gene on nasopharyngeal carcinoma CNE2 cells growth and cycle.METHODS:Three synthesized siRNA targeting PCNA gene was transfected into CNE2 cells by using LipofectamineTM reagent.The PCNA mRNA and PCNA protein were detected by real-time polymerase chain reaction(RT-PCR) and immunohistochemical method.Inverted phase contrast microscope was used to determine the CEN2 cells growth before and after PCNA-siRNA transfected.Flow cytometry was used to observe the cell cycle.RESULTS:In CNE2 cells after PCNA-siRNA transfection,the expressions of PCNA mRNA and protein were down-regulated at different degree.Inhibition ratio of PCNA mRNA was 98.5%.Meanwhile,the cell cycle was suffocated at G0/G1 stage.CONCLUSION:The synthesized PCNA-siRNA effectively interferes nasopharyngeal carcinoma cells by down-regulating the expressions of the PCNA mRNA and its protein,therefore inhibits the growth of CNE2 cells.Future application of PCNA-siRNA in the gene therapy of nasopharyngeal carcinoma might be expected.
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AIM: To evaluate the significance of cytokeratin19 (CK19) expression in diagnosing micrometastases in lymph nodes in patients with laryngeal carcinoma. METHODS: Forty cases of laryngeal carcinoma together with 163 lymph nodes were studied by the staining with hematoxylin and eosin(HE)and immunostaining with antibody against cytokeratin 19 (CK19) on histological sections of the primary tumor and regional lymph nodes. RESULTS: In all cases, CK19 was positively expressed in the primary tumor. Among 163 lymph nodes, metastases were confirmed by HE in 23 lymph nodes, and in 42 lymph nodes staining with immunohistochemistry, micrometastases were found in 19 lymph nodes. Micrometastases in the lymphonodes were significantly related to the T staging. CONCLUSION: Immunohistochemical staining is a valuable method for the detection of node micrometastases in patients with laryngeal carcinoma.
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AIM: To study the allergic disease in recurrent tonsillitis. METHODS: Immunohistochemical technique was used to investigate interleukin-4(IL-4) production in tonsil. RESULTS:The result showed that: a higher incidence of IL-4 producing cells were found in the tonsils of all ages, and a high density of IL-4 was found in the reticular crypt epithelium and extrafollicular, but low density in germinalcenter. IL-4 has been shown play a crucial role in the pathogensis of allergic disease. CONCLUSION:Allergic disease is an important reason of recurrent tonsillitis. The conclusion will give a new way to cure recurrent tonsillitis.
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AIM: To assess the effect of indomethacin on tumor invasion in a human laryngeal cancer Hep-2 cell line in vitro. METHODS: Hep-2 cells were exposed to indomethacin at different concentrations for 48 h. Then cell growth rate, the colony formation in soft agar medium and cell mobility were examined, and monolayer invasion assay was performed to assess cell invasion index. RESULTS: Preteatment with indomethacin inhibited the colony formation of Hep-2 cells and the cell mobility, and decreased the invasion index. CONCLUSION: Indomethacin can inhibit the invasion of Hep-2 cells.
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AIM: To study the expression of vascular endothelial growth factor (VEGF) in inflammatory mucosa of lacrimal sac. METHODS: Immunohistochemical S-P method was used to examine the expression of VEGF in the mocusa from 12 patients with chronic dacryocystitis and 8 volunteers. RESULTS: The positive rates of VEGF expression in different parts of the mocusa were: basal lamina: 44.3%+7.6%; surface epithelium: 16.9%?4.6%; connective tissue: 15.2%?4.9%, all normal mocusa of 8 cases were negative. There was a significant difference between the two groups (P