ABSTRACT
Excessive application of chemical fertilizer has caused many problems in Angelica dahurica var. formosana planting, such as yield decline and quality degradation. In order to promote the green cultivation mode of A. dahurica var. formosana and explore rhizosphere fungus resources, the rhizosphere fungi with nitrogen fixation, phosphorus solubilization, potassium solubilization, iron-producing carrier, and IAA-producing properties were isolated and screened in the rhizosphere of A. dahurica var. formosana from the genuine and non-genuine areas, respectively. The strains were identified comprehensively in light of the morphological characteristics and ITS rDNA sequences, and the growth-promoting effect of the screened strains was verified by pot experiment. The results showed that 37 strains of growth-promoting fungi were isolated and screened from the rhizosphere of A. dahurica var. formosana, mostly belonging to Fusarium. The cultured rhizosphere growth-promoting fungi of A. dahurica var. formosana were more abundant and diverse in the genuine producing areas than in the non-genuine producing areas. Among all strains, Aspergillus niger ZJ-17 had the strongest growth promotion potential. Under the condition of no fertilization outdoors, ZJ-17 inoculation significantly promoted the growth, yield, and accumulation of effective components of A. dahurica var. formosana planted in the soil of genuine and non-genuine producing areas, with yield increases of 73.59% and 37.84%, respectively. To a certain extent, it alleviated the restriction without additional fertilization on the growth of A. dahurica var. formosana. Therefore, A. niger ZJ-17 has great application prospects in increasing yield and quality of A. dahurica var. formosana and reducing fertilizer application and can be actually applied in promoting the growth of A. dahurica var. formosana and producing biofertilizer.
Subject(s)
Fertilizers , Rhizosphere , Angelica/chemistry , Fungi/genetics , PhosphorusABSTRACT
OBJECTIVE@#To investigate the irregular antibody positive rate and antibody specificity in children with thalassemia received long-term blood transfusion in Hainan area and analyze the causes of antibody screening positive.@*METHODS@#Micro-column gel method was used to screen the irregular antibody in 49 children who received transfusion treatment in our hospital, and the antibody specificity of the positive samples was evaluated.@*RESULTS@#Fourteen of 49 cases showed positive for screening. Among them, 11 cases showed Rh blood group antibody after detecting antibody specificity, 1 case showed the coexistence of irregular antibody and autoantibody. One case for anti-JK@*CONCLUSION@#Most of the antibodies produced after long-term blood transfusion in the children with thalassemia belong to Rh blood group antibodies; the children with mixed thalassemia are more likely to produce antibodies; the antibody screening positive rate of Li nationality is higher than that of Han nationality, which may be caused by the genetic difference of blood type between Li nationality and Han nationality.
Subject(s)
Child , Female , Humans , Infant , Male , ABO Blood-Group System , Blood Grouping and Crossmatching , Blood Transfusion , Rh-Hr Blood-Group System , beta-ThalassemiaABSTRACT
OBJECTIVE@#To compare the clinical efficacy of proximal femoral nail anti-rotation(PFNA) and dynamic hip screw(DHS) for the treatment of unstable intertrochanteric fractures in the elderly.@*METHODS@#From January 2012 to December 2015, 83 elderly patients with unstable intertrochanteric fracture of femur were divided into 45 cases treated with PFNA and 38 cases treated with DHS. In the PFNA group, there were 24 males and 21 females, aged 82 to 94(88.35±6.12) years old, duration was 1.5 to 10 (4.33±1.07) h, causes of injury involved 33 traffic accidents, 10 falls at high altitude, 2 falls; Evans-Jensen classification involved 21 cases of IIIA, 18 cases of IIIB and 6 cases of IV. In DHS group, there were 20 males and 18 females, aged 83 to 95 (88.77±5.52) years old, duration was 1 to 10 (4.37±1.05) h, 27 traffic accidents, 10 high-altitude falls and 1 fall injury, and Evans-Jensen classification involved 18 cases of IIIA, 15 cases of IIIB and 5 cases of IV. The operation time, intraoperative bleeding volume and discharged complications were observed and compared between the two groups. The Mos 36-item Short Form Health Survey (SF-36) was used to measure the quality of life before and 12 months after operation. Harris score was used to evaluate hip function 12 months after operation.@*RESULTS@#The operation time, intraoperative bleeding volume and complication rate in PFNA group were significantly lower than those in DHS group(<0.05); both groups were followed up for 12 months without falling off. The Harris score and SF-36 score at 12 months after operation in both groups were higher than those before operation(<0.05), and the excellent and good rate in PFNA group was significantly higher than that in DHS group(<0.05).@*CONCLUSIONS@#Compared with DHS, PFNA is more simple, less traumatic, less complications, better short-term survival rate and better quality of life for elderly patients with unstable intertrochanteric fracture of femur. PFNA can be used as an optimal treatment for elderly patients with unstable intertrochanteric fracture of femur.
Subject(s)
Aged, 80 and over , Female , Humans , Male , Bone Nails , Bone Screws , Femur , Hip Fractures , General Surgery , Quality of Life , Retrospective StudiesABSTRACT
This review summarizes the developmental history of natural products and research status of botanical products in different oversea countries. This paper outlines the regulation for new drug application of botanical drugs from the USA, European Union, and Japan. It also uses the Vergen (the first botanical drug approved by US FDA) as an example to discuss how to meet regulatory require-ments for new drug applications.
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OBJECTIVE:To establish a method for the determination of related substances in Cabazitaxel injection. METHODS:HPLC method was used. The determination was performed on Agilent Eclipse XDB-C18column with mobile phase consisted of water-acetonitrile-ethanol(gradient elution)at the flow rate of 1.0 mL/min. The column temperature was 35 ℃,and the detection wavelength was set at 230 nm. The sample size was 20 μ L. Established method was used to determine related substances in 3 batches of Cabazitaxel injection. RESULTS:The linear relationship of cabazitaxel were 0.039-11.60 μ g/mL(r=0.999 8,n=7). The detection limit was 2×10-4μg,and quantitation limit was 8×10-4μg. RSD of precision and reproducibility tests were all lower than 10.0%(n=6). The amount of single impurity in 3 batches of samples ranged 0.07%-0.08%,and total amount of impurities were 0.26%-0.29%. CONCLUSIONS:Established method is simple,accurate and reliable,can be used for the determination of related substances in Cabazitaxel injection.
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Objective To investigate the influence of intensified acid suppression scheme on operation-related clinical parameters, re-bleeding rate and laboratory indexes of elderly patients with ANVUGIB underwent endoscopic hemostasis. Methods 150 elderly patients with ANVUGIB underwent endoscopic hemostasis were chosen from June 2015 to June 2017 and randomly divided into control group (75 patients) with conventional dose of pantoprazole and observation group (75 patients) with intensified dose of pantoprazole; and the clinical efficacy, the disappeared time of spitting blood and melena, hemostasis time, total blood transfusion volume, hospitalization time, re-bleeding rate, conversion to open surgery rate, the levels of laboratory index in 24 h and 72 h after treatment and adverse reaction incidence of both groups were compared. Results The clinical total effects of observation group were significantly higher than that in control group (P < 0.05). The disappeared time of spitting blood and melena, hemostasis time,total blood transfusion volume, hospitalization time of observation group were significantly better than that in control group (P < 0.05). The re-bleeding rate and conversion to open surgery rate of observation group were significantly lower than that in control group (P < 0.05). The levels of laboratory index in 24 h and 72 h after treatment of observation group were significantly better than that in control group (P < 0.05). There was no significant difference in the adverse reaction incidence between the two groups (P > 0.05). Conclusion Intensified acid suppression scheme in the treatment of elderly patients with ANVUGIB undergoing endoscopic hemostasis can efficiently achieve hemostasis in early stage, relieve symptoms and signs, decrease the blood loss amount in perioperative period, reduce the conversion to open operation risk and be helpful to improve the levels of PCV and BUN.
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OBJECTIVE:To establish a method for simultaneous determination of 6 residual organic solvents in aprepitant raw material as methanol,ethanol,acetone,isopropyl alcohol,methyl tert-butyl ether and tetrahydrofuran.METHODS:Headspace capillary gas chromatography was adopted.The determination was performed on DB-624 capillary column using temperature programming.The temperature of injector port was 180 ℃,and flame ionization detector was used with temperature of 260 ℃.Nitrogen was used as carrier gas with flow rate 3.0 mL/min.The spilt ratio was 5 ∶ 1,and head-space injection volume was 1.0 mL.The head-space equilibrium temperature was set at 80 ℃,and equilibrium time was 40 min.RESULTS:The linear ranges of methanol,ethanol,acetone,isopropyl alcohol,methyl tert-butyl ether,tetrahydrofuran were 6.052-605.232 μ g/mL (r=0.999 9),9.987-998.718 μg/mL(r=0.999 9),9.998-999.768 μg/mL(r=0.999 8),9.986-998.634 μg/mL(r=0.999 9),9.991-999.090 μg/mL (r=0.999 7),1.461-146.133 μg/mL(r=0.999 5),respectively.The limits of quantitation were 1.782 1,2.079 0,0.749 8,1.777 8,0.223 1,0.607 0 μg/mL;the limits of detection were 0.594 0,0.693 0,0.249 9,0.592 6,0.074 4,0.202 3 μg/mL,respectively.RSD of precision test was lower than 2.0％.Only acetone and isopropyl alcohol were detected in stability test and reproducibility tests,RSD＜2.0％.Their recoveries were 99.34-100.75％ (RSD=0.52％,n=9),98.20％-100.24％ (RSD=0.69％,n=9),98.07％-100.07％ (RSD=0.84％,n=9),99.86％-101.32％ (RSD=0.58％,n=9),97.87％-104.02％ (RSD=2.13％,n=9),98.26 ％-100.58 ％ (RSD =0.75 ％,n =9),respectively.CONCLUSIONS:The established method is simple,accurate and reproducible,and can be used for simultaneous determination of 6 residual organic solvents in aprepitant raw material.
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Chinese medicine (CM) has been used in clinical treatment for thousands of years in China, Japan, Korea, and other countries. CM is at present attracting many attentions around the world for reproductive health care and disease prevention, including treatment of female infertility. This review focuses on the CM treatment for female infertility patients, and supplies a summary on the efficacy, safety, and mechanism of some Chinese herbal medicines, herbal medicine-derived active compounds, and acupuncture. A large number of researches have reported that CM could alleviate or even cure female infertility by regulating hormone, improving reproductive outcome of in vivo fertilization, affecting embryonic implantation, curing polycystic ovarian syndrome, endometriosis, pelvic inflammatory disease, relieving mental stress, and regulating immune system. Meanwhile, a few studies claimed that there was little adverse reaction of CM in randomized controlled trials. However, up to present there is a lack of adequate evidences with molecular mechanistic researches and randomized controlled trials to prove the CM as an effective and safe treatment for infertility. Thus, utility of CM as a complementary medicine will be a feasible method to improve the outcome of female infertility treatment.
Subject(s)
Female , Humans , Complementary Therapies , Embryo Implantation , Fertilization in Vitro , Hormones , Metabolism , Infertility, Female , Allergy and Immunology , Therapeutics , Medicine, Chinese TraditionalABSTRACT
Nine compounds were isolated from an ethanol extract of the roots of K. roxburghii by using a combination of various chromatographic techniques including column chromatography over silica gel, MCI gel, Sephadex LH-20, and reversed-phase HPLC. On the basis of physical-chemical properties and spectroscopic data analysis, their structures were identified as munjistin (1), 1-methoxy-3,6-dihydroxy-2-hydroxymethyl-9,10-anthraquinone (2), 1,2,3-trihydroxy-9,10-anthraquinone (3), arjunolic acid (4), hyptatic acid-A (5), hyptatic acid-B (6), 2α,3β,24-trihydroxyurs-12-en-28-oic acid (7), 2α,3β,23-trihydroxyurs-12-en-28-oic acid (8), and daucosterol (9). Compounds 1-9 were obtained from this genus for the first time.
Subject(s)
Anthraquinones , Chemistry , Rubiaceae , Chemistry , Triterpenes , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To explore the relationship between abdominal fat volume and obstructive sleep apnea hypopnea syndrome in obesity people.</p><p><b>METHODS</b>From July 2009 to July 2010, 50 patients with BMI > 25 were prospectively selected for study from the patients who complained of snoring in the Respiratory department. The patients were divided into OSAHS group and non-OSAHS group according to the result of sleep apnea monitoring. All the patients also received full abdominal CT and the whole abdominal fat volume was measured by 3-D CT reconstruction system. SPSS 13.0 was used for statistical analysis.</p><p><b>RESULTS</b>The whole abdominal fat volume in the two groups was analyzed by T- test, which was significantly different between the two groups (P < 0.01). It showed that there was a statistical relationship between OSAHS and abdominal fat in obesity people.</p><p><b>CONCLUSION</b>In obesity people, OSAHS has a close relationship with abdominal fat volume. The abdominal fat volume is markedly higher in OSAHS patients than that in non-OSAHS people.</p>
Subject(s)
Adult , Female , Humans , Male , Middle Aged , Abdominal Fat , Diagnostic Imaging , Case-Control Studies , Obesity , Diagnostic Imaging , Prospective Studies , Radiography , Sleep Apnea, Obstructive , EpidemiologyABSTRACT
273 bacterial strains were isolated from 20 Chinese longsnout catfish samples. The biochemical characteristics of all strains conformed to the species description of Aeromonas veronii bv. veronii on the basis of Vitek GNI+ card. Furthermore, 16S rDNA, gyrB and rpoD sequences of the representative strain PY50 were sequenced and showed high similarity with A. veronii bv. veronii in Genbank. Antibiotic-resistance of the representative strain PY50 was assessed by the Kirby-Bauer disk diffusion method, and the results showed it was susceptible and moderately susceptible to 13 and 4 of the 21 antimicrobial agents tested. Extracellular products of strain PY50 contained gelatinase, lecithinase, elastase, most of lipase and lipopolysaccharide. Virulence of strain PY50 and extracellular products to Chinese longsnout catfish were also tested, and LD50 were about 3.47~10(4) CFU per fish and 11.22 Êg per fish in intraperitoneal injection respectively. This is the first report that A. veronii bv. veronii was the pathogenic agent of ulcerative syndrome in Chinese longsnout catfish.
Subject(s)
Animals , Aeromonas/isolation & purification , Aeromonas/pathogenicity , Anti-Bacterial Agents/analysis , Enzyme Activators/analysis , Catfishes/genetics , Catfishes/microbiology , Food SamplesABSTRACT
OBJECTIVE: To synthesize a kind of novel cytotoxin with multi-drug-resistance reverting properties and study their cytotoxicity. METHODS: The target compounds were obtained from N-benzyl-4-piperidones and benzaldehyde derivatives on the condition of 20°C, 7-8 h, taking dry hydrogen chloride as catalyst, using aldol condensation reaction, inspected by TLC and purified through recrystallization. RESULTS: Five N-benzyl-3, 5-bis(arylmethylene)-4-piperidone derivatives were synthesized. The yield was over 49%. Their structures were characterized by 1H-NMR, ESI-MS with melting points. The data of cytotoxicity was obtained. CONCLUSION: The synthetic route is convenient and efficient; The compounds had good inhibiting activity for a few kinds of tumor cells. Copyright 2012 by the Chinese Pharmaceutical Association.
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<p><b>OBJECTIVE</b>To evaluate the therapeutic effect of Parkinson's disease combined with overactive bladder syndrome (GAB) treated with combined therapy of oral administration of Tolterodine with low dose and electroacuponcture.</p><p><b>METHODS</b>Sixty cases of Parkinson's disease combined with GAB were randomly divided into a combined acupuncture and medication group (group A) and a medication group (group B), 30 cases in each group. In both groups, Madopar basic doses were same, and anticholinergic agents such as Artane were stopped. In group A, Tolterodine was orally taken for 1 mg, twice a day; Baihui (GV 20), Sishengcong (EX-HN 1) and Yintang (EX-HN 3) were punctured with electroacupuncture, once a day. In group B, Tolterodine was orally taken for 2 mg, twice a day. After 6 weeks, the changes of urination and UPDRS III scores were observed, and the adverse reactions were recorded in both groups.</p><p><b>RESULTS</b>After treatment, the frequency of average urination of 24 hours, frequency of incontinence of 24 hours and average urine volume at a time were obviously improved (all P < 0. 01), of which, the above items in group A were superior to those in group B (all P < 0. 05) the UPDRSIII score in group A was superior to that in group B (P < 0.05). The adverse reactions in group A were less than those in group B.</p><p><b>CONCLUSION</b>The therapeutic effect of Parkinson' s disease combined with GAB treated with combined therapy of Tolterodine with low dose and electroacupuncture is superior to that of complete dose of Tolterodine with oral administration, with less adverse reactions. And it also can improve the motor symptom of Parkinson's disease patients.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Benzhydryl Compounds , Therapeutic Uses , Combined Modality Therapy , Cresols , Therapeutic Uses , Electroacupuncture , Parkinson Disease , Drug Therapy , Therapeutics , Phenylpropanolamine , Therapeutic Uses , Tolterodine Tartrate , Urinary Bladder, Overactive , Drug Therapy , TherapeuticsABSTRACT
<p><b>OBJECTIVE</b>To explore the efficacy and safety of endoscopic submucosal enucleation (ESE) for gastric submucosal tumors (SMTs) originated from muscularis propria.</p><p><b>METHODS</b>A total of 116 patients with gastric SMT originated from muscularis propria underwent ESE in Department of Gastroenterology of the Taizhou Hospital between July 2006 and March 2011. The occurrence of intra-operative and post-operative complications and corresponding treatment were recorded. After the treatment of ESE, the patients were followed up endoscopically.</p><p><b>RESULTS</b>The success rate of operation was 96.6%. The mean time of the procedure was (51.9±16.3) min. Complications included intra-operative bleeding (n=9, 7.8%), perforation (n=20, 17.2%), and post-operative bleeding (n=3, 2.6%). Among them, 5 cases (4.3%) required surgical intervention. None of patient had other complications such as peritoneal abscess or peritonitis. The mean hospitalization time after ESE was 6.1 days. The median follow-up period was 12 months (range, 3-48 months) and there was no residual tumor or recurrence.</p><p><b>CONCLUSION</b>ESE is a safe and feasible treatment for patients with gastric SMT originated from muscularis propria.</p>
Subject(s)
Adult , Female , Humans , Male , Middle Aged , Follow-Up Studies , Gastrectomy , Methods , Gastroscopy , Methods , Stomach Neoplasms , General Surgery , Treatment OutcomeABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression and significance of transforming growth factor-β(1) (TGF-β(1)) and Ki-67 nuclear antigen in salivary gland mucoepidermoid carcinoma.</p><p><b>METHODS</b>The expression of TGF-β(1) and Ki-67 nuclear antigen in 20 cases of salivary gland pleomorphic adenoma and 45 cases of mucoepidermoid carcinoma were detected by the streptavidin-peroxidase immunohistochemical method.</p><p><b>RESULTS</b>Expression of TGF-β(1) (39/45) and Ki-67 nuclear antigen (43/45) in mucoepidermoid carcinoma was significantly higher than in pleomorphic adenoma (P < 0.05). TGF-β(1) expression was significantly higher in moderately/poorly differentiated mucoepidermoid carcinoma (16/16) than that in well differentiated mucoepidermoid carcinoma (23/29) and pleomorphic adenoma (12/20) (P < 0.05), but not different between well differentiated mucoepidermoid carcinoma (6/23) and pleomorphic adenoma (8/12) (P > 0.05). The expression of Ki-67 was increased with the increment of TGF-β(1) expression in mucoepidermoid carcinoma (P < 0.05).</p><p><b>CONCLUSIONS</b>The high expression of TGF-β(1) may play an important role in cell differentiation and malignant proliferation of mucoepidermoid carcinoma.</p>
Subject(s)
Adolescent , Adult , Female , Humans , Male , Middle Aged , Young Adult , Adenoma, Pleomorphic , Metabolism , Pathology , Carcinoma, Mucoepidermoid , Metabolism , Pathology , Cell Differentiation , Ki-67 Antigen , Metabolism , Salivary Gland Neoplasms , Metabolism , Pathology , Transforming Growth Factor beta1 , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To evaluate the clinical effect of the disk-up sinus reamer (DSR) applied to transcrestal maxillary sinus floor elevation with simultaneous placement of implants.</p><p><b>METHODS</b>Thirty-seven patients underwent transcrestal maxillary sinus floor elevation with fifty-one implants placed simultaneously using the DSR. The residual bone height (RBH) was 3 to 8 mm, (5.61 ± 1.61) mm on average. The safety of this technique and the pain index during the operation was evaluated. The final prostheses were restored in 3-6 months postoperatively. The follow-up period was 3 to 24 months. The stability and osseointegration of the implants were clinically evaluated, and the endo-sinus bone gain around the implants were measured.</p><p><b>RESULTS</b>The elevation height ranged from 2 to 8 mm, with an average of (4.75 ± 1.55) mm. There was no detectable sinus membrane perforation, no serious suffering or uncomfortable subjective sensation in any patients during operation with a pain index of (2.22 ± 0.98). During the follow-up period, no sinus complication was observed. Favorable osseointegration was obtained. There were no implants or prostheses which were loose or lost. The survival rate was 100%. The radiographic results demonstrated that the endo-sinus bone gain tended to reach stabilization after 6 months and the marginal bone loss was (1.20 ± 0.72) mm after 12 months.</p><p><b>CONCLUSIONS</b>Transcrestal maxillary sinus floor elevation with simultaneous implant placement by DSR is a safe, invasive and handy technique, with higher elevation height, fewer clinical complications and less pain. It shows satisfactory clinical results in short term and a long-term observation is still needed.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Dental Implantation, Endosseous , Dental Implants , Follow-Up Studies , Maxillary Sinus , Diagnostic Imaging , General Surgery , Osseointegration , Pain Measurement , Radiography, Panoramic , Sinus Floor Augmentation , Methods , Tooth Loss , Diagnostic Imaging , General Surgery , Treatment OutcomeABSTRACT
<p><b>BACKGROUND AND OBJECTIVE</b>Previous studies have shown that Bmi-1 is overexpressed in a variety of tumors, suggesting that Bmi-1 plays an important role in tumorigenesis. In this study, we investigated the effect of Bim-1 siRNA on cell proliferation, cell cycle, cell apoptosis and migration of human esophageal carcinoma EC9706 cells, and explored its potential mechanisms.</p><p><b>METHODS</b>Bmi-1 small interfering RNA (siRNA) was transferred into EC9706 cells. Then, cell proliferation was measured using cell counting kit-8 (CCK-8), cell cycle and cell apoptosis were analyzed by flow cytometry, cell migration ability was detected using Boyden chamber assay, and the mRNA and protein expression levels of Bmi-1, p16, Bcl-2, Bax, and MMP-2 were determined using real-time polymerase chain reaction (PCR) and Western blot analysis, respectively.</p><p><b>RESULTS</b>Bmi-1 siRNA treatment significantly inhibited the expression of Bmi-1 at both mRNA and protein levels in EC9706 cells. Cell proliferation rate decreased dramatically in the Bmi-1 siRNA treated group than in the untreated group and in the scrambled siRNA treated group (both P < 0.001). In Bmi-1 treated group, the percentage of cells at G(0)/G(1) stage was 71.93%, which was higher than that in the untreated group (47.36%) or scramble siRNA treated group (48.47%) (both P < 0.001). Early cell apoptosis rate also increased significantly in the Bmi-1 siRNA treated group (both 17.32%) than in the untreated group (2.61%) and in the scramble siRNA treated group (2.73%) (both P < 0.001). Further experiment suggested that downregulation of Bmi-1 led to less cell migration. In EC9706 cells transfected by Bmi-1 siRNA, the expression levels of p16 and Bax increased, while the expression level of Bcl-2 decreased.</p><p><b>CONCLUSIONS</b>Bmi-1 downregulation in esophageal carcinoma cells inhibits cell proliferation, cell cycle, and cell migration, while increases cell apoptosis. These results suggest that Bmi-1 is a potential molecular target of treating esophageal cancer.</p>
Subject(s)
Humans , Apoptosis , Cell Cycle , Cell Line, Tumor , Cell Movement , Cell Proliferation , Cyclin-Dependent Kinase Inhibitor p16 , Metabolism , Down-Regulation , Esophageal Neoplasms , Metabolism , Pathology , Gene Expression Regulation, Neoplastic , Nuclear Proteins , Genetics , Metabolism , Physiology , Polycomb Repressive Complex 1 , Proto-Oncogene Proteins , Genetics , Metabolism , Physiology , Proto-Oncogene Proteins c-bcl-2 , Metabolism , RNA, Messenger , Metabolism , RNA, Small Interfering , Genetics , Repressor Proteins , Genetics , Metabolism , Physiology , Transfection , bcl-2-Associated X Protein , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To explore the indications of arthroscope for the treatment of knee osteoarthritis and investigate the correlation between knee osteoarthritis imaging and effects of arthroscope.</p><p><b>METHODS</b>From 2005.8 to 2008.4, 86 patients with knee osteoarthritis underwent arthroscope examination and treatment. Among the patients, 44 patients were male, and 42 patients were female, ranging in age from 46 to 67 years, averaged 56.3 years. Arthrodial cartilage of knee was graded by ICRS MR, and by Kellgern Laqrence X-ray. All the patients were followed up, and the duration ranged from 12 to 30 months. The Lysholm score was evaluated at the follow-up time.</p><p><b>RESULTS</b>Among 86 knees in 86 cases, cartilage injury degree of knees was graded as follows: grade 4 in 30 cases, grade 3 in 22 cases, grade 2 in 20 cases, grade 1 in 12 cases, grade 0 in 2 cases, mean grade (2.77 +/- 1.138). Postoperative Lysholm score ranged from 59 to 100, averaged (95.17 +/- 7.556), Kendall's correlation coefficient was -0.089, P = 0.317. There was no correlations between cartilage injury degree and Lysholm score. X-ray of knees was graded as follows: grade 4 in 0 cases, grade 3 in 24 cases, grade 2 in 38 cases, grade 1 in 17 cases, grade 0 in 7 cases, mean grade was (2.13 +/- 0.67), the Kendall's correlation coefficient was -0.851 with negative correlations (P = 0.036) between postoperative Lysholm score and K/L grade.</p><p><b>CONCLUSION</b>There is no correlation between the grade of knee cartilage injury confirmed by MRI (1.5T) and effects of arthroscopy, and the grade is not a gold standard as an operation indication in arthroscope procedure. The K/L grade in X-ray had important effects.</p>
Subject(s)
Aged , Female , Humans , Male , Middle Aged , Arthroscopes , Magnetic Resonance Imaging , Osteoarthritis, Knee , Diagnostic Imaging , Pathology , RadiographyABSTRACT
This study was aimed to investigate the incidence of thrombosis in patients with primary thrombocytosis (PT) and its correlation with function changes of platelets, and to explore the effect of thromboxane A2 (TXA2) inhibitor-ozagrel sodium on platelet activity and its efficacy for prevention and treatment of thrombosis. The CD62P and PAC-1 levels on platelet surface were detected by flow cytometry; the levels of TXB2 (metabolic product of TXA2) and 6-keto-PGFIalpha (metabolic product of prostacyclin) were detected by FLISA. The function change of platelets and its correlation with thrombosis were observed and compared in PT patients with and without thrombosis. The results indicated that the TXB2, PAC-1 and CD62P level, and TXB2/6-keto-PGF1alpha ratio in PT patients with thrombosis were higher than those in PT patients without thrombosis before treatment with ozagrel sodium (p<0.01). After treatment with ozagrel sodium, the function indexes of platelets such as CD62P, PAC-1, TXB2 and TXB2/6-keto-PGF1alpha except 6-keto-PGF1alpha in PT patients with and without thrombosis decreased obviously (p<0.01), but there was no significant difference in TXB2, 6-keto-PGF1alpha and TXB2/6-keto-PGF1alpha levels between PT patients with and without thrombosis except CD62P and PAC-1. It is concluded that the multi-index of platelets in PT patients with thrombosis are higher than that in PT patients without thrombosis, the activation of platelet function is a high risk factor for thrombosis of PT patients. The ozagrel sodium can obviously reduce the platelet activation, decrease the production of TXA2 and ameliorate the TXB2/6-keto-PGF1alpha ratio. The ozagrel sodium not only possesses therapeutic effect, but also preventive efficacy for thrombosis.
Subject(s)
Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Fibrinolytic Agents , Therapeutic Uses , Methacrylates , Therapeutic Uses , Thrombocythemia, Essential , Drug Therapy , Thrombosis , Drug TherapyABSTRACT
<p><b>OBJECTIVE</b>To investigate the middle and distal circum peeling with bleeding control technique for redundant prepuce.</p><p><b>METHODS</b>The middle and distal part of prepuce was circum peeled with bleeding control and electrical scalpel. More preputial inner plate was reserved.</p><p><b>RESULTS</b>From January 2004 to July 2008, 51 patients with redundant prepuce were treated with this procedure. The average blood loss was 1 to approximately 2 ml with minimal edema. The proportion of inner and outer plates was suitable.</p><p><b>CONCLUSIONS</b>This technique has the advantages of minimal blood loss and slight edema. The cosmetic result is also satisfactory.</p>