Modified TRIzol method for RNA and DNA co-extraction from blood / 法医学杂志

OBJECTIVE@#To establish a new method for RNA and DNA co-extraction from the same sample by TRIzol reagent.@*METHODS@#After the aqueous phase which contained total RNA was removed by traditional TRIzol method, the values of pH of the interphase phase and organic phase were adjusted. The DNA was precipitated with ethanol and purified with DNA IQ system. The purified DNA was measured in quality and quantity. As the template, it was amplified and typed by PCR-STR. The data was compared with that extracted by traditional TRIzol method.@*RESULTS@#The DNA extracted by this modified method showed a better result of quality and quantity than that by traditional TRIzol method and a good STR typing.@*CONCLUSION@#The modified TRIzol method is advisable and reliable to simultaneously extract both DNA and RNA from the same sample. It could be used for individual identification and paternity testing to satisfy the need of forensic science.

Serotonin, its receptors and depression / 国际药学研究杂志

Depression is an affective disorder with high prevalence. high suicide rate and low treatment rate. its neurobiologi-cal mechanism remains unclear. Most of current first-line antidepressants are based on the "monoamine hypothesis", including monoamine reuptake inhibitors, monoamine oxidase inhibitors and monoamine receptor ligand drugs. Although their therapeutic effect is definite, there are still serious defects. such as low response and delayed onset. It has been widely confirmed that depression is closely related to low serotonin (5-hydroxytryptamine, 5-HT) neurotransmission function (classical monoamine hypothesis), while modern monoamine theory suggests that the adaptability and plasticity regulation of 5-HT and its autoreceptors (such as 5-HT1A) are closely related to the treatment of depression. There are a series of new progress in recent years in the studies of 5-HT transporter proteins and 5-HT receptors, such as the positive role of S allele of the 5-HT transporter chain area in emotion and cognition, and the discoveries of the pharmacological basement of some new 5-HT-related receptor antagonists or agonists. There are also important breakthroughs in sero-tonineigic drug research and development (such as vilazodone, which approved by the US FDA in 2011, is a dual-target drug of 5-HT1A partial agonist and 5-HT reuptake inhibitor). In this review, the recent research progress on the relationship of 5-HT neuromodulation and depression is summarized. © 2006 Editorial office of Foreign Medical Sciences.